LOC105376287 as a diagnostic and therapeutic molecular marker for osteoporosis

Through the detection and inhibition of LOC105376287, the accuracy problem of early diagnosis of male osteoporosis is solved, more efficient diagnosis and treatment effects are achieved, and the ability to identify and treat osteoporosis is improved.

Active Publication Date: 2022-03-15
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plain X-ray films have low sensitivity and accuracy in diagnosing osteoporosis, and are of little help in the early diagnosis of osteoporosis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Screening for Differentially Expressed Molecules

[0063] 1. Case collection

[0064] Five elderly male patients with osteoporosis were selected, aged 62-79 years; the diagnosis of osteoporosis was based on the standards established by the Osteoporosis Committee of the Chinese Society of Geriatrics, that is, the bone mineral density at the same site was lower than the peak value of the area and decreased by two standard deviations classified as osteoporosis. During the same period, 6 healthy elderly men (healthy control group) were selected, aged 61-78 years. The selected objects exclude diabetes, primary hyperparathyroidism, hyperthyroidism, hypercortisolism, liver and kidney dysfunction, alcoholism, malnutrition, rheumatoid arthritis and other diseases that affect bone metabolism; no application within half a year Androgens, calcitonin, large amounts of calcium, bisphosphonates, and other drugs that affect bone metabolism. The two groups were comparable i...

Embodiment 2

[0105] Example 2 Large sample verification screened out differentially expressed LncRNA

[0106] 1. Sample collection

[0107] According to the method of Example 1, 32 cases of osteoporosis patients and 30 cases of male healthy controls were collected.

[0108] 2. Extract blood total RNA

[0109] Step is with embodiment 1.

[0110] 3. Validation at the RNA level

[0111] (1) Reverse transcription: According to the measured RNA concentration, 1000 ng of RNA was quantified for each sample for reverse transcription. Use the DDR037A reverse transcription kit of Dalian Biobiology, add 20ul of the system (see Table 1), add it to a 200ul enzyme-free EP tube, centrifuge and put it into a PCR instrument, and set the temperature conditions as follows: 37°C for 15min, 85°C for 5sec . After the end, the cDNA was diluted 10 times and stored at -20°C for future use.

[0112] Table 1 Reverse transcription system

[0113]

[0114] (2) Real-time quantitative PCR

[0115] Shanghai Sa...

Embodiment 3

[0128] Example 3 Effect of LOC105376287 on proliferation and differentiation of osteoblasts

[0129] 1. Interference efficiency pre-test

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PUM

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Abstract

This invention discloses that LOC105376287 can serve as a diagnostic and therapeutic molecule for osteoporosis. Through sequencing and qPCR studies, this invention revealed that the level of LOC105376287 in the blood of osteoporosis patients is significantly higher than that in normal individuals. Therefore, LOC105376287 can serve as a molecular marker for diagnosing osteoporosis. Characterizing osteoporosis using the level of LOC105376287 in the blood has the following advantages: it avoids tissue sampling and testing, reduces the suffering of subjects, and this characterization method can be used for screening healthy individuals, effectively preventing the occurrence of osteoporosis. Furthermore, in vitro experiments of this invention demonstrate that LOC105376287 expression is associated with the proliferation and differentiation of osteoblasts, a key cell in osteoporosis, and therefore can serve as a drug target for developing treatments for osteoporosis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the diagnostic application of LOC105376287, in particular to LOC105376287 as a molecule for the diagnosis and treatment of osteoporosis. Background technique [0002] The incidence of osteoporosis in men is much lower than that of postmenopausal women, so in the past, less attention was paid to men's osteoporosis than to women. Research results in recent years have shown that although young men can achieve a higher peak bone mass than women, and the start time of bone loss is also significantly later than women, but both men and women have a bone loss that is phased with age Process, that is, senile osteoporosis (type II). There are many similarities between male osteoporosis and female osteoporosis, but there are still obvious gender differences in etiology, pathology, and disease. Osteoporosis of varying degrees is common in men over 65 years old, and its serious complication is fract...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N15/113C12Q1/6883
CPCC12N15/113C12Q1/6883C12N2310/10C12Q2600/158C12Q2600/178
OwnerGUAN BOJIAN BIOTECH CO LTD