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Extraction method of jellyfish collagen

A technology of jellyfish collagen and an extraction method, applied in the field of collagen extraction, can solve the problems affecting the quality of life of patients, the burden of national medical expenses, high amputation rate, etc., and achieve the effect of good water solubility

Pending Publication Date: 2020-07-10
张 培华 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In addition, the number of diabetic patients at home and abroad has increased significantly. Due to abnormal tissue repair, serious complications such as diabetic refractory skin ulcers and wounds have been caused, leading to high amputation rates, bringing huge economic losses to patients and families, and at the same time causing national medical expenses. Serious burden; and there is still a lack of effective treatment methods in clinical practice, which greatly affects the quality of life of patients

Method used

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  • Extraction method of jellyfish collagen
  • Extraction method of jellyfish collagen
  • Extraction method of jellyfish collagen

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0052] 4. Example 1: Collagen extraction from jellyfish jellyfish in salted Zhanjiang area

[0053] (1) Wash, shred and squeeze dry, weigh 295g of salted jellyfish, soak in double distilled water, and change the water every 2 hours. After 48 hours, AgNO3 was used to detect the soaking water twice, and no white turbidity was visible to the naked eye.

[0054] (2) Crushed, filtered with four layers of gauze, drained, measured weight 414.2g, volume 425ml.

[0055] (3) Add 5 times the volume of jellyfish to soak in acetone, and divide into 3 cans: 2 cans of 150ml in group A are soaked in new acetone for 1 day, and 1 can of 125ml in group B is soaked in old acetone for 1 day.

[0056] (4) Take the sample from acetone, wash it with double distilled water until it is odorless, filter and drain, group A 100.48g, 100ml. Group B 46.75g, 50ml. Group A was soaked in 800mlna2hpo4, and group B was soaked in 400mlna2hpo4.

[0057] (5) Take samples from na2hpo4, rinse with double distille...

example 2

[0062] 5. Example 2: Collagen extraction from fresh jellyfish in Zhanjiang area

[0063] (1) Wash fresh jellyfish from Zhanjiang area with tap water 3 times, soak in double distilled water, and change the water every 2 hours. After 48 hours, use AgNO3 to detect the soaking water once, and there is no white turbidity visible to the naked eye. The wet weight was weighed to be 644.11g.

[0064] (2) Shred and crush, filter with four layers of gauze and towel, and drain. The measured weight is 77.25g and the volume is 75ml.

[0065] (3) Add 5 times the volume of acetone to soak jellyfish slurry, and stir evenly. Take the sample from acetone, wash it with double distilled water until it is odorless, filter and drain, weigh 60g and volume 70ml.

[0066] (4) Add 5 times the volume of na2hpo4 to the jellyfish slurry for soaking, and stir evenly. Take the sample from na2hpo4, rinse with double distilled water to pH7.31, filter, drain, weigh 29g, volume about 35ml.

[0067] (5) Add ...

example 3

[0071] 6. Example 3: Collagen extraction from fresh jellyfish in Zhanjiang area

[0072] (1) Wash fresh jellyfish from Zhanjiang area with tap water 3 times, soak in double distilled water, and change the water every 2 hours. After 48 hours, use AgNO3 to detect the soaking water once, and there is no white turbidity visible to the naked eye. The wet weight was weighed to be 765g.

[0073] (2) Shred and crush, filter with four layers of gauze and a towel, and drain. The measured weight is 104g and the volume is 100ml.

[0074] (3) Add 5 times the volume of acetone to soak jellyfish slurry, and stir evenly. Take the sample from acetone, wash it with double distilled water until it is odorless, filter it, drain it, weigh 130g, and weigh 125ml.

[0075] (4) Add 5 times the volume of na2hpo4 to the jellyfish slurry for soaking, and stir evenly. Take the sample from na2hpo4, rinse with double distilled water to pH7.42, filter, drain, weigh 69.31g, volume about 70ml.

[0076] (5...

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Abstract

The present invention provides an extraction method and application of jellyfish collagen. The marine organism based collagen is a biological material more advantageous than mammal collagen, and the antigenicity of the marine organism based collagen is weaker than that of the terrestrial mammal based collagen and is easy to extract. The marine organism based collagen has the higher biosafety, doesnot cause mammal based diseases and pathogen propagation risk, does not relate to religion problems and has sufficient sources. The extraction method of the jellyfish collagen mainly comprises the steps of desalination, pulping, degreasing, alkalization, enzymatic extraction, centrifugation, salt precipitation, centrifugation, precipitate redissolution, basic solution dialysis, pure water dialysis and freeze drying. The extracted collagen is in a white silk powder shape, is gathered into a white sponge state solid, is good in water solubility, and has the extremely high similarity with humancollagen in component. The prepared jellyfish collagen can be applied to the fields of products such as wound repair materials, artificial skin, beauty masks, cosmetics, implantable medical materialsand healthcare foods.

Description

technical field [0001] The invention relates to a method for extracting collagen and its application, in particular to a method for extracting collagen derived from jellyfish. The obtained jellyfish collagen has a very high similarity to human collagen in components, and has great application potential in the fields of medical treatment, beauty, health care and the like. [0002] 1. Background technology [0003] The vast majority of collagen products currently on the market are collagen extracted from the fur, tendons and other parts of terrestrial mammals (such as cattle and pigs). However, recent outbreaks of infectious diseases in terrestrial mammals such as bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE), and foot-and-mouth disease (FMD) have increased concerns about the use of terrestrial mammalian biomaterial products. And sick worry. And because of religious beliefs, Hinduism prohibits the use of bovine protein, and Islamic and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14A61K8/65A61Q19/00A61L27/24A61L27/60
CPCC07K14/78C12P21/06A61K8/65A61Q19/00A61L27/24A61L27/60
Inventor 张培华崔立峰
Owner 张 培华