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Preparation method of MOF compound biological enzyme wound healing agent

A compound biological enzyme and wound healing agent technology, applied in biochemical equipment and methods, non-active ingredients of polymer compounds, medical preparations containing active ingredients, etc., can solve problems such as lack of hydrogen peroxide and limit practical applications, and achieve promotion The effect of wound healing

Pending Publication Date: 2020-07-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the main reasons is that the optimal enzyme-like activity requires a strongly acidic environment at pH 3-4, which limits their practical application in biological systems at neutral pH, in addition to the lack of hydrogen peroxide in the biological environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Synthesis method of MOF:

[0026] (1) 0.16g of F127 and 178.3 mg of MnCl 2 Dissolve in 15ml deionized water and stir ultrasonically for 1h.

[0027] (2) Add 0.15 mL of acetic acid and stir for 1 hour.

[0028] (3) Add 60mg NH 2 -BDC was stirred for another 2 hours

[0029] (4) Transfer to an autoclave at 110° C. for 16 hours for crystallization.

[0030] (5) The obtained solid product was dissolved in water and centrifuged at 10,000 rpm to collect the precipitate, washed three times repeatedly, and stored at 4°C.

[0031] 2. Synthesis method of MOF composite biological enzyme:

[0032] (1) Dissolve the prepared 10mg MOF in 5mL water under weak magnetic stirring conditions

[0033] (2) Add 5mg GOx and stir for 12 hours

[0034] (3) Centrifuge at 10,000 rpm to collect the obtained MOF / GOx.

[0035] 3 Fibrin Gel Wraps:

[0036] (1) Dissolve 10mg of fibrinogen in 10ml of deionized water and stir for 10min.

[0037] (2) Add 10 mg MOF / GOx and stir for 3 minutes. ...

Embodiment 2

[0040] 1. Synthesis method of MOF:

[0041] (1) 0.32g of F127 and 356.6 mg of MnCl 2 Dissolve in 15ml deionized water and stir ultrasonically for 1h.

[0042] (2) Add 0.30 mL of acetic acid and stir for 1 hour.

[0043] (3) Add 120mg NH 2 -BDC was stirred for another 2 hours

[0044] (4) Transfer to an autoclave at 110° C. for 16 hours for crystallization.

[0045] (5) The obtained solid product was dissolved in water and centrifuged at 10,000 rpm to collect the precipitate, washed three times repeatedly, and stored at 4°C.

[0046] 2. Synthesis method of MOF composite biological enzyme:

[0047] (1) Dissolve the prepared 20mg MOF in 5mL water under weak magnetic stirring conditions

[0048] (2) Add 20mg GOx and stir for 12 hours

[0049] (3) Centrifuge at 10,000 rpm to collect the obtained MOF / GOx.

[0050] 3 Fibrin Gel Wraps:

[0051] (1) Dissolve 20mg of fibrinogen in 10ml of deionized water and stir for 10min.

[0052] (2) Add 20mg MOF / GOx and stir for 3 minutes....

Embodiment 3

[0055] 1. Synthesis method of MOF:

[0056] (1) 0.48g of F127 and 4.1 grams of MnCl 2 Dissolve in 15ml deionized water and stir ultrasonically for 1h.

[0057] (2) Add 0.45 mL of acetic acid and stir for 1 hour.

[0058] (3) Add 180mg NH 2 -BDC was stirred for another 2 hours

[0059] (4) Transfer to an autoclave at 110° C. for 16 hours for crystallization.

[0060] (5) The obtained solid product was dissolved in water and centrifuged at 10,000 rpm to collect the precipitate, washed three times repeatedly, and stored at 4°C.

[0061] 2. Synthesis method of MOF composite biological enzyme:

[0062] (1) Dissolve the prepared 30mg MOF in 5mL water under weak magnetic stirring condition

[0063] (2) Add 30mg GOx and stir for 12 hours

[0064] (3) Centrifuge at 10,000 rpm to collect the obtained MOF / GOx.

[0065] 3 Fibrin Gel Wraps:

[0066] (1) Dissolve 30mg of fibrinogen in 10ml of deionized water and stir for 10min.

[0067] (2) Add 30mg MOF / GOx and stir for 3 minutes....

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PUM

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Abstract

The invention relates to a preparation method of a MOF compound biological enzyme wound healing agent. The method comprises the following steps: 1) synthesizing MOF; 2) compounding MOF and a biological enzyme; and 3) performing fibrin gel wrapping. MOF can use the peroxidase-like activity function to convert H2O2 into highly toxic .OH for antibacterial application; glucose oxidase can continuouslyconvert non-toxic glucose into abundant gluconic acid and H2O2, the gluconic acid can reduce the pH value from 7 to 3-4, so that the peroxidase-like activity of the MOF can be significantly activated; H2O2 can provide the conversion raw material; and fibrin gel can effectively control the slow release of drugs to achieve the purpose of long-term treatment, and promote wound healing.

Description

technical field [0001] The invention belongs to the field of preparation of healing aids, and relates to a preparation method of a MOF composite bio-enzyme wound healing agent, in particular to a preparation method of a wound-healing agent by compounding a bio-enzyme with a natural hydrogel and MOF. Background technique [0002] Chronic wounds have gradually become a common wound type in clinical practice. If neglected, local infection may develop into systemic infection, sepsis, multiple organ dysfunction. However, traditional antibiotic treatment schemes are difficult to meet the rapidly increasing clinical needs in terms of the curative effect and research and development costs of drug-resistant bacterial infections. Therefore, it is urgent to find new antibacterial pathways and antibacterial materials. [0003] Recently, metal-organic frameworks (MOFs) have received extensive research interest, and their strong peroxidase-like activities have been explored. Although MO...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K38/36A61K9/06A61K31/80A61K38/44A61K47/42A61P17/02A61P31/04C08G83/00
CPCA61K31/80A61K38/363A61K38/4833A61K38/443C12Y304/21005C12Y101/03004A61K9/06A61K47/42A61P31/04A61P17/02C08G83/008A61K2300/00
Inventor 郑斌王树超明东甘霖
Owner TIANJIN UNIV
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