Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method

A workstation, nucleic acid technology, applied in the fields of biochemical equipment and methods, biochemical instruments, DNA preparation, etc., can solve the problems of complex DNA extraction process, contamination extraction, failure and other problems, and achieve the effect of realizing DNA extraction automation and reducing difficulty.

Pending Publication Date: 2020-07-24
广州高盛生物科技有限公司
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Problems solved by technology

[0003] In the prior art, it is usually the experimenter who puts the sample into the lysis plate, then adds the lysate, centrifuges after the sample is lysed, and then adds the lysed liquid into the binding plate for nucleic acid extraction. The entire DNA extraction The process is very complicated and requires high quality experimenters, and a little carelessness will cause pollution or extraction failure

Method used

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  • Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method
  • Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method
  • Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method

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Embodiment 1

[0043] This double extraction mode nucleic acid extraction workstation adopts the magnetic rod mode to carry out DNA extraction work, including: workbench 2, centrifugal device 3, magnetic rod device 8 and mechanical arm device 1, wherein, the workbench 2 is provided with a liquid storage tank 22 and a plate Position 21, the liquid storage tank 22 is used to place the reagents required for DNA extraction. In this embodiment, the reagents mainly stored in the liquid storage tank 22 include lysate, binding solution, washing solution and eluent; plate position 21 It is used to place the lysis plate, binding plate, cleaning plate and elution plate; in order to facilitate the storage of waste liquid, a square hole plate 7 for storing waste liquid is also arranged on the plate position 21. If the DNA needs to be detected later, place a microwell plate and a shallow well plate on the plate position 21; the centrifugal device 3 is used to separate the different products in the lysed sa...

Embodiment 2

[0062] This embodiment provides a dual-extraction mode nucleic acid extraction workstation, which works in the pipetting mode. The biggest difference between the pipetting mode and the above-mentioned magnetic wand mode is that a pipetting pump is required to use the lysate, combined with Liquid, washing liquid and eluent are added according to the extraction steps. The double extraction mode nucleic acid extraction workstation also includes a magnetic suction device, which is set on the workbench 2 and is used to absorb the magnetic beads used in DNA extraction.

[0063] In the pipetting mode, use the dual extraction mode nucleic acid extraction workstation as described above to perform DNA extraction operations, including the following steps:

[0064] S1. The pipetting pump adds the lysate located in the liquid storage tank 22 to the lysate plate that stores the sample;

[0065] S2, the gripper 11 moves the cracking plate to the centrifugal device 3 to separate the sample re...

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Abstract

The invention relates to the technical field of nucleic acid extraction, and particularly relates to a double-extraction-mode nucleic acid extraction workstation and a nucleic acid extraction method.The double-extraction-mode nucleic acid extraction workstation comprises a workbench, a centrifugal device, a magnetic rod device and a mechanical arm device, wherein a liquid storage tank and a plateposition are arranged on the workbench, the liquid storage tank is used for containing a reagent required for DNA extraction, and the plate position is used for containing a cracking plate, a combining plate, a cleaning plate and an eluting plate; the centrifugal device is used for separating different products in a cracked sample in the cracking plate under the centrifugal effect; the magnetic rod device is arranged on the workbench and used for adsorbing magnetic beads used in DNA extraction; and the mechanical arm device is arranged on the workbench, can add the reagent in the liquid storage tank into the cracking plate and can shift the cracking plate, the combining plate or the cleaning plate. The double-extraction-mode nucleic acid extraction workstation can reduce the nucleic acidextraction difficulty and ensure smooth nucleic acid extraction.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a double extraction mode nucleic acid extraction workstation and a nucleic acid extraction method. Background technique [0002] In the biological field, it is often necessary to extract the nucleic acid of the sample and then perform the test. [0003] In the prior art, it is usually the experimenter who puts the sample into the lysis plate, then adds the lysate, centrifuges after the sample is lysed, and then adds the lysed liquid into the binding plate for nucleic acid extraction. The entire DNA extraction The process is very complicated and requires high quality experimenters, and a little carelessness will cause pollution or extraction failure. [0004] Therefore, a dual extraction mode nucleic acid extraction workstation and nucleic acid extraction method are needed to solve the above technical problems. Contents of the invention [0005] The purpose of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/00C12N15/10
CPCC12N15/1013
Inventor 康贤通于瑞国孙国栋
Owner 广州高盛生物科技有限公司
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