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Primer for marking cucumber fruit shape gene, gene sequence, detection method of cucumber fruit shape and application

A detection method and technology for cucumber fruit, which can be applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as the lack of QTL sites for cloning candidate genes, etc.

Inactive Publication Date: 2020-07-24
NORTHWEST A & F UNIV
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Problems solved by technology

At present, there are as many as 19 known QTL loci related to cucumber fruit morphology (Pan et al.2019), but there is no report on the cloning of relevant QTL loci to candidate genes

Method used

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  • Primer for marking cucumber fruit shape gene, gene sequence, detection method of cucumber fruit shape and application
  • Primer for marking cucumber fruit shape gene, gene sequence, detection method of cucumber fruit shape and application
  • Primer for marking cucumber fruit shape gene, gene sequence, detection method of cucumber fruit shape and application

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Embodiment 1

[0058] This embodiment briefly introduces the process of fine mapping of the cucumber fruit shape regulation gene CsSUN, including the construction of genetic segregation populations and fine positioning processes (the mapping map is as follows: figure 1 shown).

[0059] (1) Fine mapping of cucumber fruit shape regulation gene CsSUN

[0060] In the early stage, the inventor prepared F with short fruit parent XL and long fruit parent QK as materials. 2 and F 2:3 The group conducted QTL mapping research on fruit shape, and found that two main QTL loci, CsFS1.2 and CsFS2.1, synergistically regulated fruit shape of cucumber, and the regulatory effects on phenotype were as high as 56% and 24%, respectively, and these two loci The location intervals of the points in facilities and open fields under different cultivation conditions are very consistent (Yupeng Pan, Xinjing Liang, Meiling Gao, Hanqiang Liu, Huanwen Meng, Yiqun Weng, Zhihui Cheng. Round fruit shape in WI7239 cucumber ...

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Abstract

The invention discloses a primer for marking a cucumber fruit shape gene, a gene sequence, a detection method of a cucumber fruit shape and application. The primer is specifically: CsSun-Indel_F: 5'-GAAATCCTTCTACTCCGAAACAGA-3'; CsSun-Indel_R: 5'-TTCCTCGCCTAATTTCACCAA-3'. A molecular marker CsSun-Indel and a cucumber fruit shape regulation gene CsSUN are co-separated, and the molecular marker is used for detecting and judging the long fruit shape and short fruit shape characters of a cucumber. The marker can be directly used for molecular marker assisted selection of genetic improvement of thecucumber fruit shape. The molecular marker has the advantages of simplicity, convenience, rapidness and high throughput in an assisted breeding system, so that the molecular marker has a relatively good application value in the genetic improvement of the cucumber fruit shape.

Description

technical field [0001] The invention belongs to the technical field of cucumber genetics and breeding, and in particular relates to a primer for marking cucumber fruit shape genes, a gene sequence, a method for detecting cucumber fruit shape, and an application thereof. Background technique [0002] Cucumber is one of the main vegetable crops in my country and has extremely high social value and economic benefits. Fruit shape is an important commodity trait of cucumbers, determined by the longitudinal and transverse diameters of the fruit, and is an important indicator to measure its appearance quality. In view of the different uses and eating habits, people's requirements for cucumber fruit shape vary greatly. Different consumer markets have different requirements for cucumber fruit shape (Weng et al. 2015). Therefore, it is of great significance to carry out genetic improvement of cucumber fruit shape and to breed excellent varieties with diversified fruit shape according...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 潘玉朋孟焕文程智慧
Owner NORTHWEST A & F UNIV