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Paenibacillus polymyxa agent and preparation method thereof

A technology of Bacillus polymyxa and bacterial agents, applied in the field of microbial bacterial agents, can solve the problems of harming citrus leaves, increasing the cost of pest control, affecting tree vigor, etc., to increase yield, prevent and control citrus canker, and improve disease resistance Effect

Active Publication Date: 2020-07-28
广西乐土生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus; the disease damages citrus leaves, branches and fruits, and when seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, affecting tree vigor; The fruit will drop, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit, increase the cost of disease and pest control for the fruit grower, and damage the economic benefits.
[0003] At present, citrus canker is usually prevented by spraying pesticides on citrus leaves, but on the one hand, pesticides are likely to make pathogenic bacteria resistant and reduce the difficulty of control; on the other hand, they will pollute the environment

Method used

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  • Paenibacillus polymyxa agent and preparation method thereof
  • Paenibacillus polymyxa agent and preparation method thereof
  • Paenibacillus polymyxa agent and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0033] The screening of embodiment 1 bacillus polymyxa LTPP03

[0034] 1) Sample collection: collect soil (forest land in Sitang Town, Xingning District, Nanning City), and place it in a container;

[0035] 2) Dilution, water bath heating: Take 10g of soil and put it into a 250ml beaker, add 90ml of sterile water into the beaker, shake it, and make the concentration of 10 -1 Soil dilution A; place soil dilution A in a water bath at 80°C and heat for 10 minutes;

[0036] 3) Gradient dilution:

[0037] (1) Take 3 test tubes filled with 9mL sterile water, marked as: 10 -2 、10 -3 and 10 -4 ;

[0038] (2) Shake the soil diluent A and let it rest for 30s. Use a pipette to draw 1mL of the soil diluent and add it to the water marked with 10 -2 In the test tube, gently blow and suck several times in the test tube to make it fully mixed, and then obtain the soil dilution B;

[0039] (3) Similarly, draw 1mL of soil dilution B and add the number 10 -3 In a test tube, blow and suck...

Embodiment 2

[0051] The identification of embodiment 2 bacillus polymyxa LTPP03

[0052] The China Center for Type Culture Collection identified the Bacillus polymyxa LTPP03 strain on March 15, 2019. Identification includes morphological observation of Bacillus polymyxa LTPP03 strain, physiological and biochemical characteristics test, determination and analysis of 16SrRNA gene sequence, and the taxonomic status of the strain is determined according to the identification results. The strain was identified as: Paenibacillus polymyxa LTPP03 (Paenibacillus polymyxa LTPP03). The test results of physiological and biochemical characteristics are shown in Table 1-Table 2;

[0053] Table 1 Physiological and biochemical characteristics of strain LTPP03 - enzyme activity, carbon source assimilation

[0054]

[0055]

[0056] +: Positive reaction; -: Negative reaction; w: Weak positive reaction

[0057] Table 2 Physiological and biochemical characteristics of strain LTPP03 - acid production ...

Embodiment 3

[0062] A preparation method of bacillus polymyxa bacterial agent, the preparation process is as follows:

[0063] 1) Configure medium: configure preservation medium, seed medium and fermentation medium, sterilize, and set aside;

[0064] 2) Strain activation: inoculate the LTPP03 strain into the seed slant medium for activation, and culture at 37°C for 20 hours to obtain the activated strain;

[0065] 3) Seed culture: pick the activated strain obtained in step 2) and inoculate it into 120ml of seed culture medium, and culture it with shaking at 37°C and 110r / min for 40h to obtain the seed bacterial liquid;

[0066] 4) Fermentation culture: pour the fermentation medium into the fermenter, and add the seed bacterial liquid of 4% inoculum of the fermentation medium to the fermenter, ferment and cultivate, the fermenter rotates at 110r / min, and the ventilation is 1:1. After fermenting and cultivating the seed bacterial liquid for 20 hours at a temperature of 37°C, add an organic ...

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Abstract

The invention discloses a paenibacillus polymyxa agent comprising the following effective components: paenibacillus polymyxa. The paenibacillus polymyxa is collected in the China Center for Type Culture Collection (CCTCC), with the collection number of CCTCC No: M2019116, and a 16srRNA sequence is shown in SEQ ID No.1. A preparation process comprises preparation of a culture medium, strain activation, seed culture, fermentation culture and agent preparation. The agent can effectively control citrus canker and reduce the incidence of the disease.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a Bacillus polymyxa inoculum and a preparation method thereof. Background technique [0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus; the disease damages citrus leaves, branches and fruits, and when seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, affecting tree vigor; The fruit falls, and the light one has disease scars and cannot be stored, and rots, which greatly reduces the commodity value of the fruit, increases the cost of disease and insect pest control for the fruit grower, and damages the economic benefits. [0003] At present, citrus canker is usually prevented by spraying pesticides on citrus leaves. However, on the one hand, pesticides are likely to make pathogenic bacteria resistant and reduce the difficulty of control; on the other hand, they will pollute the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/12
CPCC12N1/20C12R2001/12C12N1/205
Inventor 吕昆明廖华廖博杨瑞青王健陈裕新黄一帆
Owner 广西乐土生物科技有限公司
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