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Pearl protein and preparation method and application thereof, and cosmetic containing pearl protein

A pearl protein and cosmetic technology, which is applied to the preparation methods of peptides, cosmetics, cosmetic preparations, etc., can solve the problems of organic solvent residue, unfavorable skin absorption, poor permeability, etc., achieves good whitening and anti-aging functions, and is beneficial to the skin. Good absorption and permeability

Pending Publication Date: 2020-07-28
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the research on the deep processing of existing pearl products is not sufficient. The pearl components in whitening skin care products are often produced by relatively extensive hydrolysis methods, such as using some strong acidic substances for hydrolysis, and the products obtained are often not conducive to skin absorption. , and the permeability is not good, the whitening and anti-aging effect is not ideal; at the same time, these pearl components decomposed by strong acid and other substances will also have the problem of residual organic solvents, and it is easy to affect the environment

Method used

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  • Pearl protein and preparation method and application thereof, and cosmetic containing pearl protein
  • Pearl protein and preparation method and application thereof, and cosmetic containing pearl protein
  • Pearl protein and preparation method and application thereof, and cosmetic containing pearl protein

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preparation example Construction

[0054] According to one aspect of the present invention, a preparation method of pearl protein, the preparation method comprises the following steps:

[0055] (a), providing an aqueous suspension containing pearl powder, and then hydrolyzing the aqueous suspension under the action of protease to obtain a hydrolyzate A;

[0056] (b), the hydrolyzate A obtained in step (a) is hydrolyzed under the action of lactic acid to obtain hydrolyzate B;

[0057] (c), performing solid-liquid separation and desalination treatment on the hydrolyzate B obtained in step (b) in sequence to obtain pearl protein.

[0058] The preparation method of pearl protein provided by the present invention comprises the following steps: (a), providing an aqueous suspension containing pearl powder, and then hydrolyzing the aqueous suspension under the action of protease to obtain a hydrolyzate A; (b ), hydrolyzing the hydrolyzate A obtained in step (a) under the action of lactic acid to obtain hydrolyzate B; ...

Embodiment 1

[0106] A preparation method of pearl protein, said preparation method comprising the following steps:

[0107] (a) Mix pearl powder with a particle size of 5 μm with water at a material-to-liquid ratio of 1:30 g / ml, boil for 15 minutes, and then cool to 40° C. to obtain a water suspension;

[0108] Subsequently, under the condition of 40°C, adding 0.15 g of protease per 1 g of pearl powder, adding neutral protease to the aqueous suspension for hydrolysis twice, each time for 2 to 4 hours, to obtain hydrolyzate A;

[0109] The mass ratio of the protease added for the first time to the protease added for the second time is 0.1:0.05;

[0110] (b), the temperature of the hydrolyzate A is increased to 95°C, and 2ml of lactic acid is added to the hydrolyzate A at a ratio of 2ml of lactic acid per 1g of pearl powder, and the hydrolyzate is hydrolyzed for 2 hours to obtain the hydrolyzate B;

[0111] (c) Use a 200mm Buchner funnel to suction-filter the hydrolyzate B, and then use a d...

Embodiment 2

[0115] A preparation method of pearl protein, said preparation method comprising the following steps:

[0116] (a) Mix pearl powder with a particle size of 20 μm with water at a material-to-liquid ratio of 2:30 g / ml, boil for 20 minutes, and then cool to 45° C. to obtain a water suspension;

[0117] Subsequently, under the condition of 45° C., at the ratio of 0.75 g of protease per 2 g of pearl powder, neutral protease was added to the aqueous suspension for hydrolysis twice, each time for 4 hours, to obtain hydrolyzate A;

[0118] The mass ratio of the protease added for the first time to the protease added for the second time is 0.5:0.25;

[0119] (b), the temperature of the hydrolyzate A is raised to 100°C, and 4.5ml of lactic acid is added to the hydrolyzate A at a ratio of 4.5ml of lactic acid per 2g of pearl powder, and the hydrolyzate is hydrolyzed for 2.5h to obtain the hydrolyzate B;

[0120] (c) Use a 300mm Buchner funnel to suction-filter the hydrolyzate B, and the...

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Abstract

The invention provides a pearl protein and a preparation method and application thereof, and a cosmetic containing the pearl protein, and relates to the technical field of deep processing of pearl products. The preparation method comprises the following steps: (a), providing an aqueous suspension containing a pearl powder, and then hydrolyzing the aqueous suspension under the action of protease toobtain a hydrolyzed solution A; (b), hydrolyzing the hydrolyzed solution A obtained in the step (a) under the action of lactic acid to obtain a hydrolyzed solution B; and (c), sequentially performingsolid-liquid separation and desalination treatment on the hydrolyzed solution B obtained in the step (b) to obtain the pearl protein. The preparation method uses the protease and lactic acid to sequentially hydrolyze the aqueous suspension containing the pearl powder to prepare the pearl protein, and the pearl protein prepared by the preparation method is more easily absorbed by the skin. At thesame time, the preparation method of the invention does not have the problem of organic solvent residue after the hydrolyzed solution B is treated by the filtering and desalting method, and thereby isvery suitable for industrialized large-scale production.

Description

technical field [0001] The invention relates to the technical field of deep processing of pearl products, in particular to pearl protein, its preparation method and application, and cosmetics containing it. Background technique [0002] Conchiolin protein (Conchiolin protein) is a unique hard protein in a class of aquatic products. It is often closely linked with calcium in the shell to form a lustrous nacre. The nacre is hydrolyzed to produce hydrolyzed conchiolin protein. Hydrolyzed conchiolin is also often named after specific materials, such as hydrolyzed pearl protein, hydrolyzed oyster protein, etc. [0003] Pearl is an ancient organic gemstone, mainly produced in pearl shellfish and mother-of-pearl molluscs. Pearls have always been regarded as precious treasures. They are not only precious decorations for women, but also precious beauty products and herbs. It has long been recorded in "Compendium of Materia Medica" that pearls have the functions of clearing heat an...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/435C07K1/12C07K1/34C07K1/14A61K8/64A61Q19/02A61Q19/08
CPCA61K8/64A61Q19/02A61Q19/08C07K14/43504C12P21/06
Inventor 郭朝万宫村公纪旷小珊聂艳峰
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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