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A preparation method and fermentation medium of a Shenzimycin fermentation medium

A fermentation medium and the technology of Shenzimycin, applied in the field of pesticides, can solve the problems of low potency, high cost, unfavorable industrialization development, etc., and achieve the effect of eliminating antagonism and improving resource utilization

Active Publication Date: 2022-03-18
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microbial fermentation is a common method for the production of agricultural antibiotics. It is effective and environmentally friendly. However, the titer of Shenzimycin for industrial production in the world is still very low (about 3000mg / L), and the cost is relatively high, which is not conducive to the development of industrialization.

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  • A preparation method and fermentation medium of a Shenzimycin fermentation medium
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  • A preparation method and fermentation medium of a Shenzimycin fermentation medium

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Effect test

Embodiment 1

[0033] A high-yielding shenzamycin fermentation medium formula and preparation method, the specific steps are as follows:

[0034](1) Preparation of soybean powder and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The amino acid liquid is distilled under reduced pressure to obtain a dechlorinated hydrolyzed amino acid liquid. The vacuum distillation steps are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid steaming speed slows down, and after recovery, a dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (dry basis of the remaining material) is obtained.

[0035] (2) Preparation of fermentation medium: soybean powder 5g / L, corn steep liquor 5g / L, soybean meal dechlorination hydrol...

Embodiment 2

[0040] A high-yielding shenzamycin fermentation medium formula and preparation method, the specific steps are as follows:

[0041] (1) Preparation of soybean flour and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The hydrolyzed amino acid liquid is distilled under reduced pressure to obtain the dechlorinated hydrolyzed amino acid liquid. The steps of vacuum distillation are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid distilling speed slows down, and after recovery, soybean meal dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (the remaining material on a dry basis) is obtained.

[0042] (2) Preparation of fermentation medium: soybean powder 8g / L, corn steep liquor 11g / L, so...

Embodiment 3

[0048] A high-yielding shenzamycin fermentation medium formula and preparation method, the specific steps are as follows:

[0049] (1) Preparation of soybean flour and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The hydrolyzed amino acid liquid is distilled under reduced pressure to obtain the dechlorinated hydrolyzed amino acid liquid. The vacuum distillation steps are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid steaming speed slows down, and after recovery, a dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (dry basis of the remaining material) is obtained.

[0050] (2) Preparation of fermentation medium: soybean powder 10g / L, corn steep liquor 16g / L, soybean meal dechlo...

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Abstract

The invention discloses a method for preparing a fermentation medium of Shenzimycin and the fermentation medium. After the raw materials are processed and prepared according to the medium formula, water is added and evenly placed in a fermentation tank. Cooling; add the Shenzimycin fermentation strain into the fermenter under aseptic conditions, cultivate it at 26-28°C and 200-300r / min for 68-72 hours, and then purify to obtain high-yield Shenzimycin; Among them, the medium formula is: soybean powder 5-10g / L, corn steep liquor 5-16g / L, plant cake hydrolyzed amino acid solution 20-30g / L, glucose 10-15g / L, glycerin 20-25g / L, grain Sodium phosphate 5~15g / L, the solvent is water. The production capacity of the shenzimycin in the fermentation medium of the invention is increased by 2.4 to 2.75 times, and the recovery rate of the shenzimycin reaches 78% to 80%.

Description

technical field [0001] The invention belongs to the technical field of pesticide technology, and in particular relates to a preparation method and a fermentation medium of a Shenzimycin fermentation medium. Background technique [0002] Shenzimycin is a new type of microbial source pesticide (agricultural antibiotic), which has the characteristics of high efficiency, safety, broad spectrum and environmental friendliness. Its main component is phenazine-1-carboxylic acid (phenazine-1-carboxylic acid, PCA ), Shenzimycin can effectively control the fungal, bacterial and nematode diseases of various crops such as rice, wheat, fruits and vegetables, and at the same time promote the growth of plants. Mymycin can control 75% to 80% of watermelon wilt and 73% to 80% of sweet pepper blight. It has been fully affirmed by the National Agricultural Technology Extension Service Center and relevant provincial and municipal agricultural plant protection departments. Under the background o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/12C12N1/38C12R1/39
CPCC12P17/12C12N1/20C12N1/38
Inventor 于秋生何剑飞於慧利韩粉丽冯镇泰张红艳郝祥蕊
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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