Primers for detecting rabbit hemorrhagic disease viruses and nucleic acid detection method

Abstract

The invention belongs to the technical field of molecular biology, and relates to a biological detection technology, in particular to a specific primer for detecting rabbit hemorrhagic disease virus and an MFIA xTAG detection method. The specific primer for detecting the RHDV is a specific primer designed according to a VP60 gene sequence of the RHDV, and on the basis of the properties of the specific primer, a liquid chip detection method suitable for rapidly and accurately detecting the RHDV is further optimized and established. Compared with a traditional detection method, the method integrates the RT-PCR and liquid chip detection, so that the use amount of detection samples is small, the lowest virus detection amount is 0.1 LD50, the sensitivity is 100 times that of traditional RT-PCR,operation is easy and rapid, the detection cost can be greatly reduced, and rapid and accurate detection of RHDV is achieved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and relates to a biological detection technology, in particular to a specific primer for detecting rabbit haemorrhagic disease virus and a detection method of MFIA xTAG. Background technique [0002] Rabbit hemorrhagic disease, also known as rabbit plague, rabbit viral hemorrhagic pneumonia, rabbit hemorrhagic pneumonia, or rabbit viral sepsis, is a severe infectious disease of rabbits caused by rabbit viral hemorrhagic disease virus (RHDV) , which is characterized by strong infectivity, congestion and hemorrhage in parenchymal organs, and extremely high morbidity and mortality. Rabbit hemorrhagic disease only occurs among rabbits. Rabbits over 3 months old are the most susceptible. It often shows explosive epidemics and causes devastating losses to the rabbit breeding industry. [0003] RHDV is a member of the genus Lagovirus in the caliciviridae family, and is related to European hare...

AI Technical Summary

Problems solved by technology

However, these methods generally have problems such as poor specificity, time-consuming, low sensitivity, and false positives, which make pathogenic diagnosis always a difficult problem. Therefore, it is particularly important to find a fast and accurate method for diagnosing RHDV virus infection.

[0004] RT-PCR technology is a common technical method in the field of biotechnology at present. It has the advantages of strong specificity and high sensitivity. However, the result judgment needs to rely on electrophoresis measurement, which is not only time-consuming and laborious, but also the reaction product is prone to contamination, resulting in false positive results.

Images