New applications of neuropeptide y

A neuropeptide, inflammatory response technology, applied in the field of medicine, can solve the problems of high incidence and mortality of heart failure, and achieve the effect of reducing myocardial infarction area, slowing myocardial remodeling, and improving cardiac dysfunction.

A neuropeptide, inflammatory response technology, applied in the field of medicine, can solve the problems of high incidence and mortality of heart failure, and achieve the effect of reducing myocardial infarction area, slowing myocardial remodeling, and improving cardiac dysfunction.

CN111494606BActive Publication Date: 2021-12-14GUANGZHOU MEDICAL UNIV +1
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  • New applications of neuropeptide y
  • New applications of neuropeptide y

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Experimental materials

[0067] (1) 129S2 / SvPasCrl mice: aged from 8 to 10 weeks, with a body weight of 20g to 22g; all operations on the experimental 129S2 / SvPasCrl mice were performed in accordance with the guidelines for the feeding and use of experimental animals issued by the National Institutes of Health ( National Institutes of Health publication number 85-23, revised 1996).

[0068] (2) Anesthetic and sedative drugs: ketamine (50mg / kg) and xylazine (50mg / kg).

[0069] (3) Neuropeptide Y (Neuropeptide Y, NPY) powder: purchased from Sigma, USA, prepared with 0.9% by mass saline. The preparation concentration of this embodiment includes 12.5 μg / kg, 25 μg / kg, 50 μg / kg and 100 μg / kg.

[0070] 2. Experimental method

[0071] (1) Mouse anesthesia:

[0072] Take 129S2 / SvPasCrl mice, inject ketamine (50 mg / kg) and xylazine (50 mg / kg) intraperitoneally according to the weight of the mice, and anesthetize the 129S2 / SvPasCrl mice.

[0073] (2) Construction of model ...

Embodiment 2

[0089] 1. Experimental materials

[0090] (1) 129S2 / SvPasCrl mice: aged from 8 to 10 weeks, with a body weight of 20g to 22g; all operations on the experimental 129S2 / SvPasCrl mice were performed in accordance with the guidelines for the feeding and use of experimental animals issued by the National Institutes of Health ( National Institutes of Health publication number 85-23, revised 1996).

[0091] (2) Anesthetic and sedative drugs: ketamine (50mg / kg) and xylazine (50mg / kg).

[0092] (3) Neuropeptide Y (Neuropeptide Y, NPY) powder: purchased from Sigma, USA, prepared with 0.9% by mass saline. The preparation concentration of this embodiment includes 12.5 μg / kg, 25 μg / kg, 50 μg / kg and 100 μg / kg.

[0093] 2. Experimental method

[0094] (1) Mouse anesthesia:

[0095] Take 129S2 / SvPasCrl mice, inject ketamine (50 mg / kg) and xylazine (50 mg / kg) intraperitoneally according to the weight of the mice, and anesthetize the 129S2 / SvPasCrl mice.

[0096] (2) Construction of model ...

Embodiment 3

[0112] 1. Experimental materials

[0113] (1) 129S2 / SvPasCrl mice: aged from 8 to 10 weeks, with a body weight of 20g to 22g; all operations on the experimental 129S2 / SvPasCrl mice were performed in accordance with the guidelines for the feeding and use of experimental animals issued by the National Institutes of Health ( National Institutes of Health publication number 85-23, revised 1996).

[0114] (2) Anesthetic and sedative drugs: ketamine (50mg / kg) and xylazine (50mg / kg).

[0115] (3) Neuropeptide Y (Neuropeptide Y, NPY) powder: purchased from Sigma, USA, prepared with 0.9% by mass saline. The preparation concentration of this embodiment includes 12.5 μg / kg, 25 μg / kg, 50 μg / kg and 100 μg / kg.

[0116] 2. Experimental method

[0117] (1) Mouse anesthesia:

[0118] Take 129S2 / SvPasCrl mice, inject ketamine (50 mg / kg) and xylazine (50 mg / kg) intraperitoneally according to the weight of the mice, and anesthetize the 129S2 / SvPasCrl mice.

[0119] (2) Construction of model ...

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Abstract

The invention relates to a new application of neuropeptide Y, which is mainly used for preparing the drug for myocardial remodeling after acute myocardial infarction. Verified by acute myocardial infarction model mice, neuropeptide Y can improve cardiac dysfunction, reduce myocardial infarct size, reduce myocardial fibrosis, reduce inflammation, and reduce myocardial cell structure damage, thereby reducing the mortality rate of acute myocardial infarction. Myocardial remodeling plays a protective role in myocardial infarction mice.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a new application of neuropeptide Y. Background technique [0002] Acute myocardial infarction is a common heart disease that rapidly causes extensive ischemic necrosis of the myocardium due to vascular blockage, often accompanied by severe morbidity and mortality, threatening human health and quality of life. Myocardial fibrosis, also known as myocardial calcification, refers to the excessive accumulation of collagen fibers and a significant increase in collagen concentration in the normal tissue structure of the myocardium. It is the main pathophysiological process of reverse myocardial remodeling after acute myocardial infarction, which can eventually lead to left heart failure insufficiency and heart failure. Reverse myocardial remodeling that occurs after acute myocardial infarction remains a major cause of morbidity and mortality. Therefore, reverse myocardial remodelin...

Claims

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Application Information

Patent Timeline
14 Dec 2021
Publication
CN111494606B
IPC
A61K38/17; A61P9/10
CPC
A61K38/17; A61P9/10
Inventors
余细勇; 蓝辉耀