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A kind of natural active water-soluble protein and polypeptide and preparation method thereof

A water-soluble protein and protein technology, applied in the field of natural active water-soluble protein and polypeptide and its preparation, to achieve the effects of easy mastering, improvement of sub-health status, and promotion of absorption

Inactive Publication Date: 2011-12-21
刘长福
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the existing problems of polypeptides, the purpose of the present invention is to provide a production method of polypeptides with a new structure and concept

Method used

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  • A kind of natural active water-soluble protein and polypeptide and preparation method thereof
  • A kind of natural active water-soluble protein and polypeptide and preparation method thereof
  • A kind of natural active water-soluble protein and polypeptide and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The mass ratio fraction of the present embodiment is as follows:

[0048] Natural protein-rich raw material: 100 parts of selected silkworm chrysalis dried products;

[0049] Protein extractant: 800 parts of 1% sodium hydroxide solution;

[0050] Degreasing agent: 250 parts of cyclohexane

[0051] Deionized water: 1000 parts

[0052] Delipase: Candida lipolytica lipase 0.01 parts

[0053] Decellulase: 0.05 parts of cellulase

[0054] Protective capping modifier: 0.1 parts of iodoacetic acid

[0055] Local acidifier: 30 parts of 0.03N hydrochloric acid

[0056] Acid protease: pepsin 0.4 part

[0057] Neutral protease: 0.3 parts of papain

[0058] Decolorizer: 20 parts of activated carbon

[0059] Deodorant: homemade deodorant A5 parts

[0060] The reaction steps and specific operations of the present embodiment are as follows:

[0061] (1) get the selected silkworm chrysalis dry product that mass proportion is 100 parts, add the 1% sodium hydroxide solution of ...

Embodiment 2

[0070] The mass ratio fraction of the present embodiment is as follows:

[0071] Natural protein-rich raw materials: 100 servings of selected dried earthworm products;

[0072] Protein extractant: 600 parts of 1% potassium hydroxide solution;

[0073] Degreasing agent: No. 6 solvent oil 200 parts

[0074] Deionized water: 800 parts

[0075] Delipase: Candida lipolytica lipase 0.04 parts

[0076] Decellulase: 0.02 parts of cellulase

[0077] Protective capping modifier: 0.1 parts of sodium metabisulfite

[0078] Local acidifier: 20 parts of 0.03N hydrochloric acid

[0079] Acid protease: pepsin 0.2 part

[0080] Neutral protease: trypsin 0.1 parts

[0081] Decolorizer: 10 parts of self-made color adsorbent

[0082] Deodorant: 2 parts of homemade deodorant B

[0083] The reaction steps and specific operations of the present embodiment are as follows:

[0084] (1) get the selected house earthworm dry product that mass proportion is 100 parts, add the 1% potassium hydrox...

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Abstract

The invention is: a natural active water-soluble protein and polypeptide and a preparation method thereof. Using selected natural protein-rich raw materials, through grinding and pulping, using a protein extractant to extract crude protein, using a solvent for simple degreasing, and then enzymatic fine degreasing and enzymatic fine decellulose to obtain refined protein pulp, and then Use end-capping agents to protect useful protein end groups, then use local acidifiers to locally acidify the long chain of proteins, then use acid protease for preliminary enzymatic hydrolysis, neutral protease for deep enzymatic hydrolysis, and timely detect the molecular weight, molecular weight distribution and amino acids of water-soluble proteins and polypeptides Content and other control indicators, when the control indicators reach the required value, stop the enzymatic hydrolysis reaction, then add deodorizing agent to remove odor, decolorizing agent to decolorize, filter and separate through filter, freeze or spray dry to obtain the finished product of natural active water-soluble protein and polypeptide .

Description

[0001] Field [0002] The invention belongs to the new technology of biological products, relates to the fields of natural active plant hormones, feed additives, food additives, health care products and medical treatment, and in particular relates to a natural active water-soluble protein and polypeptide and a preparation method thereof. technical background [0003] The 1986 Nobel Prize in Physiology was awarded to Rita Levi-Mont.alcini and Stanley Co-hen for their discovery of polypeptide growth factors (NGF and EGF) , for opening a new field of fundamental scientific research of broad importance. Polypeptides are biologically active substances involved in various cellular functions in living organisms. With the rapid development of molecular biology and biochemical technology, the study of peptides has made amazing and epoch-making progress. It has been found that there are tens of thousands of peptides existing in organisms, and it is found that all cells can synthesize p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 刘长福何向东刘昕
Owner 刘长福
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