Biological gasoline detergent synergist
A bio-gasoline and synergist technology, applied in the field of microorganisms in clean energy, can solve the problems of microbial preparations that do not have the cleaning and dispersing effect of gasoline, and achieve the effects of reduced sediment quality, low fuel consumption, and convenient use
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Embodiment 1
[0036] A bio-gasoline detergent synergist, characterized in that:
[0037] Contains Rhodococcus erythropolis T7-3 and Serratia marcescens CGMCC4074.
[0038] The preparation method of above-mentioned biological gasoline cleaning agent, its step is as follows:
[0039] (1) Inoculate Rhodococcus erythropolis T7-3 and Serratia marcescens CGMCC 4074 in glycerol cryopreservation tubes, pick 3 rings and transfer them to 80mL LB shaker flasks, at 65°C, 120rpm , shaking culture for 24h, and finally configured to prepare 10 8 CFU / ml bacterial solution, and then mix it evenly according to the mass ratio of 1:1 to become a mixed bacterial solution;
[0040] (2) Gasoline induced culture:
[0041] Transfer to gasoline induction medium, inoculum amount 10%, shaking culture for 24 hours;
[0042] The induction medium:
[0043] 1% gasoline, 1.5% yeast powder, 1% sucrose, 0.5% trehalose, 0.1% sodium dihydrogen phosphate, 0.1 potassium dihydrogen phosphate, pH 7.2;
[0044] (3) After indu...
Embodiment 3
[0068] The modified clean gasoline obtained in Example 1-2 was used as the experimental group. The unmodified 93# gasoline was used as the control group.
[0069] Use BYD 476ZQA gasoline engine to compare the combustion chamber deposit quality and fuel consumption rate of each group.
[0070] Experimental conditions: each experimental group is equipped with 50 liters of 93# gasoline, at room temperature 20 ℃, air pressure 101.7kPa, humidity 60RH%, start the engine, enter the real-time measurement and control system, adjust the engine speed to 2000r / min, load In the working condition of 75%, after 100 hours of operation, the deposit quality and fuel consumption rate of each group of combustion chambers were compared. Each experiment was repeated 3 times. The specific test results are shown in Table 1.
[0071] Table 1 Results of combustion chamber deposits and fuel consumption rate of each group
[0072] Combustion chamber deposits / mg Fuel consumption rate / g(kWh) ...
Embodiment 4
[0075] The bio-gasoline detergent synergist obtained in Example 1 and Comparative Examples 1-3 is used as an experimental group, and Shell SL5W / 30 is used as a comparative example to directly carry out the four-ball test abrasion test of gasoline engine oil to compare the lubricating effect of the bio-detergent . The test data are shown in Table 2:
[0076] Table 2 Four-ball test wear scar test
[0077] Wear spot diameter (mm) Example 1 group 0.657 Comparative example 1 group 1.813 Comparative example 2 groups 1.636 Comparative example 3 groups 1.798 control group 0.389
[0078] From the data analysis of the wear spot diameter test: the control group has the worst effect. Compared with the comparative example group, the diameter of the wear spot of the example group is obviously reduced, and the wear resistance is increased. Although relative to the SL5W / 30 lubricating oil, there is a certain gap between the effects of the embod...
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