Improved chicken-derived antimicrobial peptides and their coding genes, recombinant plasmids, recombinant bacteria and applications
A technology of recombining plasmids and coding genes, which is applied in the field of genetic engineering, can solve the problems of low antibacterial ability of natural antimicrobial peptides and limit the wide application of antimicrobial peptides, and achieve the effect of enhanced antibacterial ability and strong antibacterial ability
Active Publication Date: 2021-12-10
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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Problems solved by technology
However, natural antimicrobial peptides are not perfect, and most of them have low antibacterial ability, which greatly limits the wide application of antimicrobial peptides
Method used
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Embodiment 1
[0029] 1. The modified chicken-derived antimicrobial peptide DNA sequence was introduced into the enzyme cutting site
[0030] The coding gene of the improved antimicrobial peptide (the first gene SEQ ID NO.4 and the second gene SEQ ID NO.5), the 5' ends were respectively introduced into the restriction enzyme site XhoI Reintroduce the DNA sequence of the Kex2 restriction site (amino acid restriction site EKR) 3' introduced stop codon TGA, plus XbaI Restriction sites. The designed sequence was submitted to a DNA synthesis company (Nanjing KingScript Biotechnology Co., Ltd.) for synthesis, and the synthesized DNA sequence was connected to the plasmid pUC57-Simple (mass spectrum as shown in figure 1 shown) above.
[0031] The nucleotide sequence of the first gene after introducing the restriction site is shown in SEQ ID NO.6, specifically:
[0032]
[0033] The nucleotide sequence of the second gene after introducing the restriction site is shown in SEQ ID NO.7, specifi...
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The invention provides improved chicken-derived antimicrobial peptides and their coding genes, recombinant plasmids, recombinant bacteria and applications, and belongs to the technical field of genetic engineering. The improved chicken-derived antimicrobial peptides include the first antimicrobial peptide and / or the second antibacterial peptide; The amino acid sequence of the first antibacterial peptide is shown in SEQ ID NO.2; the amino acid sequence of the second antibacterial peptide is shown in SEQ ID NO.3. The improved chicken-derived antimicrobial peptide of the present invention is an improved chicken-derived antimicrobial peptide designed on the basis of the original chicken-derived antimicrobial peptide (the amino acid sequence is shown in SEQ ID NO.1). Compared with the original chicken-derived antimicrobial peptide, the improved chicken-derived antimicrobial peptide of the present invention has significantly enhanced antibacterial ability.
Description
technical field [0001] The invention relates to the technical field of genetic engineering, in particular to improved chicken-derived antimicrobial peptides and their coding genes, recombinant plasmids, recombinant bacteria and applications. Background technique [0002] Antimicrobial peptides are biologically active small molecule polypeptides induced in organisms, usually composed of 12 to 45 amino acids, with a molecular weight of about 40kD, and are not easy to make bacteria resistant to drugs; in addition, they also have broad-spectrum antibacterial properties, Thermal stability, high water solubility and no residue. Therefore, the study and application of antimicrobial peptides as non-toxic, non-polluted, non-drug-resistant new antibacterial agents to replace antibiotics is of great significance for the protection of human health. However, natural antimicrobial peptides are not perfect, and most of them have low antibacterial ability, which greatly limits the wide app...
Claims
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IPC IPC(8): C07K14/465C12N15/12C12N15/81C12N1/19A61K38/17A61P31/04C12R1/84
CPCC07K14/465C12N15/815A61P31/04A61K38/00
Inventor 王东升张国华黄江丽圣平王汝霞张志红于一尊计少石
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI