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Microbial agent JF for preventing and treating corn stalk rot in saline-alkali soil and preparation method of microbial agent JF

A technology of microbial agent and corn stalk, which is applied in the field of microorganisms to achieve the effects of convenient transportation, lower ear weight, and lower soil pH

Active Publication Date: 2020-08-18
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few microbial inoculants that can be used for the prevention and treatment of corn diseases at present, and it is necessary to develop microbial inoculants suitable for the prevention and treatment of corn stalk rot

Method used

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  • Microbial agent JF for preventing and treating corn stalk rot in saline-alkali soil and preparation method of microbial agent JF
  • Microbial agent JF for preventing and treating corn stalk rot in saline-alkali soil and preparation method of microbial agent JF
  • Microbial agent JF for preventing and treating corn stalk rot in saline-alkali soil and preparation method of microbial agent JF

Examples

Experimental program
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Effect test

preparation example Construction

[0030] The present invention provides a kind of microbial inoculant JF and preparation method thereof for preventing and treating corn stalk root disease, comprising the following steps:

[0031] Preparation of 1S microbial inoculum powder: seed the single colonies of activated Sonora desert Bacillus B-2, Bacillus paralicheniformis B-26, Sonora desert Bacillus N-16 and Bacillus pumilus N-17 Cultivation and fermentation culture, the four kinds of cultured bacterial liquids are adsorbed by adsorbents to obtain microbial inoculum powder; the four kinds of microbial inoculum powders are mixed according to the mass ratio of 1:1:1:1 to obtain a mixed powder; wherein, the The four strains mentioned above are all preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC), and the preservation date is March 5, 2020; the preservation number of Sonora desert Bacillus B-2 is CGMCC NO.19454, deputy The preservation number ...

Embodiment

[0041] (1) Compatibility between halophilic and alkaline antagonistic bacteria

[0042] Referring to the determination method of Barbosa, inoculate one functional strain on the solid medium plate of Horikoshi I or modified LB-I or modified LB-II medium, and inoculate another functional strain on the other half vertically. Each treatment was repeated 3 times. Place the plate in a biochemical incubator at 30°C for cultivation, observe and record the compatibility reaction between the two strains after 24 hours. The two strains that did not produce antibacterial bands were considered compatible, otherwise they were considered incompatible. Compatibility results see figure 1 .

[0043] Horikoshi I medium (g / L): glucose 10.0g, peptone 5.0g, yeast extract 5.0g, K 2 HPO 4 1.0g, MgSO 4 0.2g, Na 2 CO 3 10.0g (sterilized separately), 15-20g agar, pH 9.0, sterilized at 121°C for 20min.

[0044] Improved LB-I medium (75.0g / L NaCl): tryptone 10.0g, yeast extract 5.0g, NaCl 75.0g...

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Abstract

The invention provides a preparation method of a microbial agent JF for preventing and treating corn stalk rot. Characterized in that it is characterized in that it comprises, the method comprises thefollowing steps: 1, preparation of 1S microbial agent powder; the preparation method comprises the following steps: adding activated bacillus sonorensis B-2, 3, 4-thiadiazole into a fermentation tank; the bacillus licheniformis strain is prepared from bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26, bacillus licheniformis B-26 andbacillus licheniformis B-26. The preparation method comprises the following steps: respectively carrying out seed culture and fermentation culture on single colonies, namely bacillus solani N-16 andbacillus pumilus N-17, and adsorbing the four cultured bacterial solutions by using an adsorbent, so as to obtain microbial agent powder; mixing the four microbial agent powders according to a mass ratio of 1: 1: 1: 1 to obtain a mixed powder; and 2, preparation of a 2S microbial agent JF: mixing the microbial agent powder prepared in step 1 with auxiliary materials to prepare the microbial agentJF. The microbial agent JF prepared by the invention can effectively improve saline-alkali soil and prevent corn stalk rot of the saline-alkali soil.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial agent JF for preventing and treating corn stalk rot in saline-alkali land and a preparation method thereof. Background technique [0002] As an important land resource, saline-alkali land improvement and utilization have always been the focus and hotspot in the field of agricultural research, and it is also the basis for the development of reserve resources of cultivated land and the potential guarantee for increasing grain production in my country. The global saline-alkali land area accounts for 10% of the world's total area. In China, the total area of ​​saline-alkali land that affects cultivated land exceeds 500 million mu. The area of ​​saline-alkali land ranks third among all countries in the world. Among them, the area with agricultural development potential accounts for the total area of ​​China's cultivated land. More than 10%. The pH value of saline-a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCC12N1/20A01N63/00
Inventor 沙月霞周涛张丽霞任剑孙娇方秋香李明洋聂小雁邢敏
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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