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Preparation method for preparing corn peptide by heat treatment assisted enzyme method

A technology of corn peptide and auxiliary enzyme, which is applied in the direction of fermentation, etc., can solve the problems of high molecular weight of corn peptide and different product quality, and achieve the effect of simple operation

Pending Publication Date: 2020-08-18
YUCHENG BAOLIKANG BIOLOGICAL FEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common pretreatment methods include ultrasonic, microwave, high pressure, etc., but after practical use and research, there are various problems in the current pretreatment methods, and the quality of the obtained products is different, and the molecular weight of the obtained corn peptide is relatively large At present, there is a lack of a method to improve the yield of corn peptide and ensure the quality of corn peptide product

Method used

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  • Preparation method for preparing corn peptide by heat treatment assisted enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1: Weigh 100g of corn gluten powder, crush and refine it, pass through a 40-mesh sieve for later use, and continue crushing particles with a particle size larger than 40-mesh sieve until all of them pass through a 40-mesh sieve, then add the crushed corn gluten powder to 733.3ml of distilled water Mix fully and evenly in the mixture to form a corn gluten powder solution with a solid content of corn gluten powder of 12%;

[0019] Step 2: Heat the corn gluten powder solution obtained in Step 1 to 115°C and keep it warm for 30 minutes;

[0020] Step 3: After heating in step 2, cool down to 55°C, add 0.8g of alkaline protease, 0.8g of neutral protease, and 0.8g of flavor protease, and perform enzymolysis reaction for 7 hours under stirring conditions;

[0021] Step 4: Dry the mixed liquid after the enzymolysis reaction in Step 3 at a temperature of 135° C. for 2.7 hours to obtain the corn peptide product.

Embodiment 2

[0023] Step 1: Weigh 100g of corn gluten powder, crush and refine it, pass through a 40-mesh sieve for later use, and continue crushing particles with a particle size larger than 40-mesh sieve until all of them pass through a 40-mesh sieve, then add the crushed corn gluten powder to 566.7ml of distilled water Mix well and evenly to form a corn gluten powder solution with a solid content of corn gluten powder of 15%;

[0024] Step 2: Heat the corn gluten powder solution obtained in Step 1 to 115°C and keep it warm for 40 minutes;

[0025] Step 3: After heating in step 2, cool down to 55°C, add 0.8g of alkaline protease, 0.8g of neutral protease, and 0.8g of flavor protease, and perform enzymolysis reaction for 8 hours under stirring conditions;

[0026] Step 4: Dry the mixed solution after the enzymolysis reaction in Step 3 at a temperature of 135° C. for 2 hours to obtain the corn peptide product.

Embodiment 3

[0028] Step 1: Weigh 100g of corn gluten powder, crush and refine it, pass it through a 40-mesh sieve for later use, and continue crushing particles with a particle size larger than 40 mesh sieve until all of them pass through a 40-mesh sieve, then add the crushed corn gluten powder to 900ml of distilled water to fully evenly Mixing and being configured into corn gluten powder solution with a solid content of corn gluten powder of 10%;

[0029] Step 2: Heat the corn gluten powder solution obtained in Step 1 to 115°C and keep it warm for 25 minutes;

[0030] Step 3: After heating in step 2, cool down to 55°C, add 0.6g of alkaline protease, 0.6g of neutral protease, and 0.6g of flavor protease, and perform enzymolysis reaction for 7 hours under stirring conditions;

[0031] Step 4: Dry the mixed liquid after the enzymolysis reaction in Step 3 at a temperature of 135° C. for 2.5 hours to obtain the corn peptide product.

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Abstract

The invention relates to a preparation method for preparing corn peptide by a heat treatment assisted enzyme method, which comprises the following steps: step 1, crushing and refining a crude productof corn protein powder, adding distilled water into the crushed corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution; 2, heating the corn protein powder solution obtained in the step 1 to 90-120 DEG C, and performing heat preservation for 20-40 min; 3, after heating in the step 2 is completed, cooling to 50-60 DEG C, adding protease, and carrying out an enzymolysis reaction for 4-8 hours under a stirring condition; and 4, drying the mixed solution subjected to the enzymolysis reaction in the step 3 at 125-150 DEG C for 2-3 hours to obtain the corn peptide product. The distilled water content of the obtained corn peptide is smaller than or equal to 5%, the crude protein content is larger than or equal to 55%, and the content of small peptide with themolecular weight being 1000 Da or below is larger than or equal to 90%. The quality of the corn peptide can be ensured while the yield of the corn peptide is increased.

Description

technical field [0001] The invention belongs to the field of corn peptide preparation, and in particular relates to a heat treatment-assisted enzymatic method for preparing corn peptide and a preparation method thereof. Background technique [0002] Corn gluten powder is commonly known as corn yellow powder, and its protein content is about 70%. After distillation and hydrolysis with protease, corn peptide with small molecular weight can be obtained. These corn peptides have the functions of providing essential amino acids and nutrients for the body, easy to absorb, improving immunity, lowering blood pressure, and improving the digestion and absorption rate of the body. These functions and advantages make corn peptide a research hotspot. [0003] Because the yield of the current method for producing corn peptide is low, and the protein content of corn peptide is low, a pretreatment method is used for corn gluten powder to improve the enzymatic hydrolysis efficiency and the ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘峰袁学宾姚康涂强王娜娜梁淑淑刘亚琦
Owner YUCHENG BAOLIKANG BIOLOGICAL FEED CO LTD
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