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A method for separating Gelcyne alkaloid monomers from Gelzia alkaloids by combining high-speed countercurrent chromatography with preparative liquid chromatography

A technology of high-speed countercurrent chromatography and alkaloids, which is applied in the field of separation of active ingredients of natural medicines to achieve the effects of strong flexibility and controllability, shortened separation time and less sample loss

Active Publication Date: 2021-06-08
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] In view of the defects existing in the prior art, the object of the present invention is to provide a method for rapidly and efficiently separating and preparing various Gelcinnas alkaloid monomers from Gelkins alkaloids by using a combination of high-speed countercurrent chromatography and preparative liquid chromatography. method, this method overcomes the defect that the general method in the prior art is difficult to simultaneously separate a variety of gelzia alkaloid monomers

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  • A method for separating Gelcyne alkaloid monomers from Gelzia alkaloids by combining high-speed countercurrent chromatography with preparative liquid chromatography
  • A method for separating Gelcyne alkaloid monomers from Gelzia alkaloids by combining high-speed countercurrent chromatography with preparative liquid chromatography
  • A method for separating Gelcyne alkaloid monomers from Gelzia alkaloids by combining high-speed countercurrent chromatography with preparative liquid chromatography

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Embodiment 1

[0024] A high-speed counter-current chromatography combined with preparative liquid chromatography to separate the method for the alkaloids from the alkaloids:

[0025] 1. Sample: Gelkins alkaloids, which is the solvent extract of Gelkins fresh medicinal materials in Longtou Town, Liucheng County, Liuzhou City, Guangxi, according to the traditional method for extracting Gelkins alkaloids (Xu Jian, Liang Chongzhen, Extraction method Comparison of [J], Chinese Journal of Pharmaceutical Sciences. 341-342 (1988); Zhang Lanlan, master, First Military Medical University, (2005); Xiao Sa, master, Hunan Agricultural University, (2016)). The chemical reagents used are commercially available analytically pure chemical reagents.

[0026] 2. Instrument: analytical liquid chromatograph (equipped with SPD-M20A detector, LC-20AT infusion pump, CBM-20A system controller, SIL-20A autosampler, Shimadzu Corporation of Japan); TBE-300A high-speed Countercurrent chromatography (inner diameter 1.6...

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Abstract

The invention discloses a method for separating glyphosate alkaloid monomers from glyph alkaloids in combination with high-speed countercurrent chromatography and preparative liquid chromatography. After the individual components were separated, high-speed countercurrent chromatography was used for primary separation and preparative liquid chromatography for secondary separation, and the target Gelcyon alkaloid monomer components were collected respectively. The method uses a plurality of chromatographs in combination to separate glyphosate monomers, which has the advantages of large amount of preparation, short cycle time, high purity, etc., can simultaneously separate and obtain 8 glyph alkaloid monomers, has controllability and automatic productivity, and It has broad application prospects.

Description

technical field [0001] The present invention relates to a method for separating Gelzia alkaloid monomers from Gelzia alkaloids, in particular to a method for separating and preparing Gelzia alkaloids monomers from Gelzia alkaloids by using high-speed countercurrent chromatography and preparative liquid chromatography. A body method belongs to the technical field of separation of active ingredients of natural medicines. Background technique [0002] Gelsemium elegans, also known as heartbroken grass, big tea medicine, cannon leaf, yellow flower bitter vine, yellow mangcai, etc., is an evergreen woody vine of the genus Gelsemium elegans, produced in Jiangxi, Fujian, Taiwan, Hunan, In Guangdong, Hainan, Guangxi, Guizhou, Yunnan and other provinces and regions, it has the effects of reducing swelling and pain, removing poison and killing insects. It is often used as a traditional Chinese veterinary herbal medicine. It has the effect of repelling insects on pigs, cattle, and shee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/22C07D459/00
CPCC07D459/00C07D491/22
Inventor 刘兆颖陆英唐其
Owner HUNAN AGRICULTURAL UNIV
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