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A method for preparing shellfish high f-value oligopeptides using metal-organic framework materials

A technology of metal-organic framework and organic framework, which is applied in the direction of biochemical equipment and methods, immobilized on or in inorganic carriers, enzymes, etc., can solve the problem of long time required for removal of aromatic amino acids, low product yield, production issues such as rising costs

Active Publication Date: 2020-10-27
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In traditional enzymatic hydrolysis methods, proteases cannot be reused, resulting in higher production costs
At present, the removal methods of aromatic amino acids mainly include activated carbon method and membrane filtration method. In comparison, the activated carbon method is widely used because of its low cost and convenient operation, but it lacks specificity and takes time to remove aromatic amino acids. Long, and will cause the loss of certain branched chain amino acids, resulting in lower production efficiency and lower product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A method for preparing shellfish high F-value oligopeptides using metal-organic framework materials, using the processing by-products of Chlamys farreri as raw materials, comprising the following steps:

[0055] 1. Raw material pre-treatment: shellfish processing by-products are cleaned and crushed and homogenized in a conditioner.

[0056] 2. Preparation of metal-organic framework-immobilized enzymes:

[0057] (1) Purification of pepsin: Dissolve 0.1 g of pepsin in 2 mL of PBS buffer (pH 6.8) at room temperature, then centrifuge at 8000 r / min for 10 min, take the supernatant, and adjust the pH value to the isoelectric point of pepsin 1.5, then slowly add ammonium sulfate to 60% saturation, put it in a refrigerator at 4°C for 12 hours, and centrifuge at 8000 r / min for 10 minutes; take the precipitate and dialyze at 4°C for 24 hours, freeze-dry to obtain a solid powder;

[0058] (2) Purification of flavor protease: Dissolve 0.1 g flavor protease in 2 mL Tris buffer (pH8...

Embodiment 2

[0078] The difference from Example 1 is that pepsin and flavor protease are not purified, and the immobilized enzymes are directly prepared;

[0079] All the other technical features are the same as in Embodiment 1.

Embodiment 3

[0081] The difference from Example 1 is that pepsin and flavor protease are not purified and immobilized, and commercial enzymes are directly used for enzymatic hydrolysis (in shellfish enzymatic hydrolysis, Pepsin / ZIF-8 is replaced by commercial pepsin, Flavor / ZIF -8 is replaced by commercial flavored protease, and the enzyme activity per unit raw material remains unchanged);

[0082] All the other technical features are the same as in Embodiment 1.

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PUM

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Abstract

The invention discloses a method for preparing shellfish high F-value oligopeptides by using metal organic framework materials, which includes enzymatic hydrolysis of shellfish, preparation of specific magnetic metal organic framework materials and removal of aromatic amino acids; the specific magnetic metal In the preparation of organic framework: with copper as the metal center and 1,3,5-benzenetricarboxylic acid as the organic ligand, Fe 3 o 4 Magnetic microspheres to obtain a magnetic metal-organic framework material that specifically adsorbs aromatic amino acids; in the removal of aromatic amino acids: the product obtained by enzymatic hydrolysis of shellfish is treated with a magnetic metal-organic framework that specifically adsorbs aromatic amino acids , to remove aromatic amino acids to obtain high F value oligopeptides. The invention applies the metal-organic framework material to the preparation of high F value oligopeptides, and realizes the efficient preparation of high F value oligopeptides and the efficient improvement of F value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing shellfish high F-value oligopeptides by using metal organic framework materials. Background technique [0002] The production of high F-value oligopeptides is an oligopeptide mixture composed of 2-9 amino acid residues with high content of branched-chain amino acids (BCAA) and low content of aromatic amino acids (AAA) formed by degrading food protein by enzymatic hydrolysis technology. Because of its special amino acid composition, it can improve the protein nutritional status of patients with liver disease, has the functions of auxiliary treatment of hepatic encephalopathy, anti-fatigue, treatment of phenylketonuria and hangover, and has potential liver protection and liver protection effects. [0003] Enzymatic hydrolysis and removal of aromatic amino acids are two key steps in the production of high F value oligopeptides. In traditional enzymati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12N11/14C12N11/089C12N9/50
CPCC12N9/50C12N11/089C12N11/14C12P21/06
Inventor 刘海梅赵芹裴继伟姜雯李海燕张莉莉刘兆胜
Owner LUDONG UNIVERSITY