Terahertz imaging-based biomacromolecule content relative quantitative detection method

A biomacromolecule and terahertz imaging technology, applied in the field of biomacromolecule detection, can solve the problems of complex material operation, time and labor cost, and achieve the effect of saving time and labor cost, detection speed and accuracy, and good economy.

Pending Publication Date: 2020-08-25
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for relative quantitative detection of biomacromolecules based on terahertz imaging, which can solve the defects of existing methods for quantitative and qualitative

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  • Terahertz imaging-based biomacromolecule content relative quantitative detection method

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Embodiment Construction

[0037] The embodiments of the present invention are described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the drawings provided in the following embodiments are only used to illustrate the basic idea of ​​the present invention in a schematic manner, and the following embodiments and features in the embodiments can be combined with each other without conflict.

[0038] Among them, the accompanying drawings are only used for exemplary description, and represent only schematic diagrams, not physical drawings, and should not be ...

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Abstract

The invention relates to a terahertz imaging-based biomacromolecule content relative quantitative detection method, and belongs to the field of biomacromolecule detection. The method comprises the following steps: S1, obtaining a nitrocellulose film or a polyvinylidene fluoride film containing biomacromolecules; S2, placing the to-be-detected film on a terahertz time-domain spectrometer, and carrying out scanning imaging to obtain a time-domain spectrum of a point; S3, performing Fourier transform and smoothing processing on the time domain spectral data, and taking the processed time domain spectral data as frequency domain spectral data; S4, taking N points on each to-be-detected film, measuring the thickness of the to-be-detected film, and taking a mean value as the thickness of the to-be-detected film; and S5, calculating, analyzing and recombining the frequency domain spectral data of each pixel point of the measurement film, and converting the frequency domain spectral data intogray values of the corresponding pixel points, so as to form a visual image reflecting the spatial distribution condition of the biomacromolecule content on the film. The method has the advantages ofno need of antibodies, simple operation, fast and accurate detection speed, time saving, labor cost saving, and realization of label-free detection.

Description

technical field [0001] The invention belongs to the field of biological macromolecule detection, and relates to a relative quantitative detection method of biological macromolecule content based on terahertz imaging. Background technique [0002] The commonly used biomolecular detection technology is immunostaining (Western Blot), which is an experimental method commonly used in molecular biology, biochemistry and immunogenetics. The basic principle is to stain cells or biological tissue samples processed by gel electrophoresis with specific antibodies. Information on the expression of a specific protein in the cell or tissue being analyzed is obtained by analyzing the location and depth of staining. [0003] The antigen-antibody reaction brings better specificity, but at the same time it also brings more complicated operations and the attendant material, time and labor costs. Moreover, for some proteins for which high-quality antibodies cannot be obtained, the accuracy of...

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Application Information

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IPC IPC(8): G01N21/3586G01N33/68
CPCG01N21/3586G01N33/68
Inventor 颜识涵汤明杰杨忠波张明焜张华崔洪亮
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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