Method for preparing PHA from corn steep liquor as raw material through fermentation

A technology for corn steep liquor and enzymatic hydrolysis of corn, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of hindering the PHA process, expensive fermentation raw materials, and high fermentation costs, reducing dosage and solving emissions. problems, the effect of improving fermentation efficiency

Active Publication Date: 2020-08-28
COFCO NUTRITION & HEALTH RES INST +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the expensive fermentation raw materials and high fermentation costs faced by the synthesis of PHA through microbial fermentation have greatly hindered the process of PHA replacing cheap traditional petroleum-based plastics.

Method used

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  • Method for preparing PHA from corn steep liquor as raw material through fermentation

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preparation example Construction

[0024] The preparation method of the enzymolyzed corn steep liquor comprises: enzymatically hydrolyzing corn steep liquor to obtain an enzymolyzed product, and then subjecting the enzymatically hydrolyzed product to solid-liquid separation to obtain the enzymatically hydrolyzed corn steep liquor;

[0025] Wherein, the enzymes used in the enzymatic hydrolysis are cellulase, hemicellulase, amylase and acid protease.

[0026] Corn steep liquor is one of the by-products of the corn deep processing industry. Corn steeping water is concentrated to obtain rice syrup. Corn steep liquor is a highly nutritious substance, and when the corn steep liquor is concentrated to a solid content of 40-50% by weight, the protein content will exceed 40% by weight. In addition, corn steep liquor also contains a variety of amino acids, vitamins, inorganic salts phosphorus, potassium and other nutrients.

[0027] Corn steep liquor is widely used. Corn steep liquor and corn husks are mixed and process...

Embodiment approach

[0029] According to a preferred embodiment of the present invention, the solid content of the corn steep liquor is 10-50% by weight (for example, 10% by weight, 15% by weight, 20% by weight, 25% by weight, 30% by weight, 35% by weight, 40% by weight, 45% by weight, 50% by weight, or any value in between), more preferably 40-50% by weight.

[0030] According to the present invention, the corn steep liquor can be any corn steep liquor that meets the above requirements in terms of solid content.

[0031] According to the present invention, the corn steep liquor may be corn steep liquor after impurity removal, or corn steep liquor without impurity removal. Preferably, the corn steep liquor is corn steep liquor after impurity removal.

[0032] According to the present invention, preferably, the impurity removal method is filtering.

[0033] More preferably, the filtering is selected from at least one of screen filtering, gauze filtering and vacuum filtering.

[0034] The present ...

preparation example 1

[0098] This preparation example is used to illustrate the activation of fermentation strains

[0099] Seed medium: containing 5g / L yeast powder, 10g / L peptone and 60g / L sodium chloride.

[0100] Halomonas was inoculated in the seed medium for primary activation culture at 37°C and 200rpm, and cultivated to OD 600 Reach about 4, obtain first-grade seed liquid;

[0101] The primary seed solution was inoculated into the seed medium with an inoculation amount of 10% by volume, and the secondary activation culture was carried out at 37°C and 200rpm, and cultivated to OD 600 Reach about 4, obtain secondary seed liquid, obtain fermented seed liquid.

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Abstract

The invention relates to the field of PHA fermentation, in particular to a method for preparing PHA from corn steep liquor as a raw material through fermentation. The method comprises the following step of inoculating a PHA fermentation strain into a fermentation culture medium for fermentation under the condition that the PHA can be produced by fermentation. A nitrogen source of the fermentationculture medium is provided by enzymolyzed corn steep liquor. A preparation method of the enzymolyzed corn steep liquor comprises the following steps of carrying out enzymolysis on the corn steep liquor to obtain an enzymolysis product, and then carrying out solid-liquid separation on the enzymolysis product to obtain the enzymolyzed corn steep liquor. Enzymes used in the enzymolysis are cellulase,hemicellulase, amylase and acid protease. According to the method, the production cost of the PHA can be reduced to a certain extent, the fermentation efficiency is improved, the discharge of the corn steep liquor is reduced, and the added value of the corn steep liquor is improved.

Description

technical field [0001] The invention relates to the field of PHA fermentation, in particular to a method for preparing PHA by fermenting corn steep liquor as a raw material. Background technique [0002] Over the past half-century, global plastic production has soared from 15 million tons in 1964 to 322 million tons in 2015, for a total of 7.8 billion tons. And since 2015, more than 300 million tons of plastic waste has flowed into the environment every year. Although a series of "plastic restriction orders" have been issued around the world, "white pollution" has intensified. PHA (Polyhydroxybutyrate, polyhydroxybutyrate) is a bio-polyester synthesized by microorganisms, and its properties are similar to traditional petroleum-based plastics such as polyethylene and polypropylene. At the same time, it is considered to be a green substitute for traditional petroleum-based plastics because of its good biodegradability. However, the expensive fermentation raw materials and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12N1/20C12R1/01
CPCC12N1/20C12P7/625
Inventor 佟毅李义袁恺刘安妮彭超陈博周卫强李大勇刘海军吴琼陈国强叶健文
Owner COFCO NUTRITION & HEALTH RES INST
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