Primer composition, reagent, detection method and system for realizing accurate typing of vitamin D receptor Fok1 site based on probe method

Abstract

The invention provides a primer composition, reagent, detection method and system for realizing accurate typing of a vitamin D receptor Fok1 site based on a probe method. The primer composition for realizing accurate typing of the vitamin D receptor Fok1 site based on the probe method is primers and probes which are designed according to the polymorphism of the vitamin D receptor gene Fok1 (rs2228570) SNP site. The reagent, detection method and system for realizing accurate typing of the vitamin D receptor Fok1 site based on the probe method are a reagent, detection method and system respectively adopting the primer composition for realizing accurate typing of the vitamin D receptor Fok1 site based on the probe method. The primer composition for realizing accurate typing of the vitamin D receptor Fok1 site based on the probe method provided by the invention solves the technical problems such as expensive equipment, aerosol pollution, and tedious steps in the Fok1 site polymorphism detection in the prior art.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a primer composition, a reagent, a detection method and a system for realizing accurate typing of the vitamin D receptor Fok1 site based on a probe method. Background technique [0002] Vitamin D receptor gene (vitamin D (1,25-dihydroxyvitamin D3) receptor, VDR), located at 12q13.11, with a total length of 63.5kb and 11 exons, the mRNA length is 4,775nt, encoding 428 amino acid residues Constituent vitamin D receptor (VDR) protein, the nuclear hormone receptor for vitamin D3, is also a secondary receptor for gallstone acid. VDRs are transcriptional regulators, members of the steroid hormone / thyroid hormone receptor superfamily, that function as ligand-inducible replication factors. The protein regulates gene expression mainly through a series of metabolic response pathways, including immune response pathways and tumor activation pathways. [0003] A number of domestic and foreign ...

AI Technical Summary

Problems solved by technology

There is the same problem of aerosol pollution; and in the result judgment process, gel electrophoresis is required, which involves many cumbersome links such as gel preparation, sample spotting, gel running, imaging, etc., which are prone to errors and require more manpower; and The results are presented in the form of pictures, and the interpretation process relies on subjective factors, which is prone to errors

Images