Immunoturbidimetry detection reagent and preparation method thereof

A detection reagent and immunoturbidimetric technology, which is applied in the field of biochemical analysis, can solve the problems of large deviation of detection results and low detection accuracy, and achieve the effect of increasing absorbance, improving sensitivity, and avoiding detection deviation

Pending Publication Date: 2020-08-28
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of immune turbidimetric detection that can solve the aforementioned problems in view of the existing immunoturbidimetric method detection reagents described in the background technology, when testing low values, the detection result deviation is large, the detection accuracy is low, etc. Reagent

Method used

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  • Immunoturbidimetry detection reagent and preparation method thereof
  • Immunoturbidimetry detection reagent and preparation method thereof
  • Immunoturbidimetry detection reagent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Immunoturbidimetric detection reagent, which includes latex microspheres, buffer, ε-polylysine, activator and preservative.

[0030] Activators included N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride at a concentration of 50 mg / ml.

[0031] The buffer was MES buffer, the pH of the buffer was 6.0, and the molar mass of the buffer was 20 mM.

[0032] The mass percentage of the preservative is 0.05%, and the preservative is sodium azide.

Embodiment 2

[0034] On the basis of Example 1, the difference from Example 1 is that the concentration of the activator is 100 mg / ml; the pH of the buffer is 8.0, and the molar mass of the buffer is 50 mM; the mass percentage of the preservative is 0.1%, and the preservative is The agent is thimerosal.

Embodiment 3

[0036] On the basis of Example 1, the difference from Example 1 is that the concentration of the activator is 200 mg / ml; the pH of the buffer is 7.0, and the molar mass of the buffer is 100 mM; the mass percentage of the preservative is 0.15%, and the preservative is The agent is Proclin-300.

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Abstract

The invention discloses an immunoturbidimetry detection reagent and a preparation method thereof. The reagent comprises latex microspheres, a buffer solution, [epsilon]-polylysine, an activating agentand a preservative, wherein the activating agent is prepared from N-hydroxyl succinimide and 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride, and the pH value of the activating agent is7 to 9. The concentration of the activating agent is 50-300mg/ml, the pH value of the buffer solution is 6.0-8.0, the molar mass of the buffer solution is 20-100mM, and the mass percent of the preservative is 0.05-0.2%. According to theimmunoturbidimetry detection reagent,by optimizing the components and proportion of the reagent and treating the latex microspheres before coupling, the sensitivityof the latex microspheres is improved, and the absorbance of some detection items at a low value is improved, so that the detection deviation caused by the fact that the real condition of a sample cannot be accurately reflected when the low value is tested is avoided, and the detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to an immunoturbidimetric detection reagent. Background technique [0002] Serum immunological examination is a physiological function test that the body recognizes "self" and "non-self" antigens, forms natural immune tolerance to autoantibodies, and rejects "non-self" antigens. The in vitro diagnostic reagent of the immunization method uses this principle to detect antibodies or antigens in clinical serum or body fluids with known antigens or antibodies in vitro. Different detection methods in antigen-antibody binding have different characterizations, which are used to calculate the quantification in detection, such as absorbance, luminescence and other parameters. [0003] Immunolabeling technology refers to labeling a known antigen or antibody on an easily displayed substance, and calculating the status of the antigen-antibody reaction by detecting the label, thereb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531
CPCG01N33/54393G01N33/54346G01N33/531
Inventor 贾引军金君玉赵年福张辉王明伟吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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