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Composition high in free amino acids and use as a raw material and complete feed for animal feeding

A technology of free amino acids and amino acids, applied in the field of animal feed raw materials and complete feeds, can solve the problems of amino acid denaturation, destruction, and high cost, and achieve the effect of easy digestion

Pending Publication Date: 2020-09-04
布列塔尼精细化工公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Indeed, from an industrial point of view, it is technically difficult and expensive to obtain keratin hydrolysates with very high levels of free amino acids
Additionally, excessive hydrolysis risks denaturing and destroying amino acids

Method used

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  • Composition high in free amino acids and use as a raw material and complete feed for animal feeding
  • Composition high in free amino acids and use as a raw material and complete feed for animal feeding
  • Composition high in free amino acids and use as a raw material and complete feed for animal feeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Example 1 - Hydrolyzate 1

[0142] Preparation of hydrolyzate 1

[0143] 4500 kg of poultry feathers were introduced into a 20000 liter reactor / hydrolysis tower. The first chemical hydrolysis step was carried out by adding 18000 liters of hydrochloric acid (24%), the hydrolysis was carried out at 72° C. for 4.5 hours. The resulting product was stored at ambient temperature for 48 hours (pause in between). Next, a second chemical hydrolysis was performed by heating at 107 °C for 6 hours without addition of acid. The resulting product was left to cool. Cystine and tyrosine were extracted by precipitation with sodium hydroxide at pH 4 to 5. The hydrolyzate is desalted by electrodialysis. 22000 kg of hydrolyzate 1 were obtained in liquid form.

Embodiment 2

[0144] Embodiment 2-hydrolyzate 2

[0145] Preparation of hydrolyzate 2

[0146] 1 kg of desalted hydrolyzate 1 was diluted with 19.2 kg of purified water and passed through a nanofiltration membrane calibrated at 100 to 250 Da at a flow rate of 400 L / h at a pressure of 20 bar. The collected retentate weighed 5.2 kg and the permeate weighed 14.55 kg. The incoming desalted hydrolyzate contained 93% free amino acids (ie 0.19 kg) and the outgoing permeate contained 99.06% free amino acids, ie 0.05 kg. Therefore, the yield of free amino acids was 26.4%.

Embodiment 3

[0147] Embodiment 3-hydrolyzate 3

[0148] Preparation of hydrolyzate 3

[0149] 1 kg of desalinated hydrolyzate 1 was diluted with 12.6 kg of purified water and passed through a nanofiltration membrane calibrated at 100 to 250 Da at a flow rate of 800 L / h at a pressure of 36 bar. The collected retentate weighed 5.2 kg and the permeate weighed 8.55 kg. The incoming desalted hydrolyzate contained 93% free amino acids (ie 0.19 kg) and the outgoing permeate contained 97.87% free amino acids, ie 0.04 kg. Therefore, the yield of free amino acids was 22.4%.

[0150] result

[0151] Table 1 shows the free amino acid / total amino acid weight fraction for each amino acid present.

[0152] [Table 1]

[0153]

[0154] *The percentage of amino acids in free form in the hydrolyzate is less than 0.002%.

[0155] Determination of the composition of the hydrolyzate 2

[0156] The amino acid of hydrolyzate 2 was determined according to the method adapted and adjusted according to EC R...

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Abstract

A hydrolysate including at least 94%, preferably at least 96%, more preferably 100% by weight of free amino acids relative to the total weight of the amino acids of the hydrolysate, the hydrolysate including at least the following amino acids: at least 93%, preferably at least 95%, of valine in free form by weight relative to the total weight of valine in the hydrolysate, at least 90%, preferablyat least 95%, of isoleucine in free form by weight relative to the total weight of isoleucine in the hydrolysate, at least 95%, preferably 100%, of leucine in free form by weight relative to the totalweight of leucine in the hydrolysate. Also, a method for preparing the hydrolysate and also a starting material and a complete feed for animal feed containing the hydrolysate.

Description

technical field [0001] The present invention relates to the field of compositions having a high content of free amino acids, in particular compositions obtained from keratin hydrolysates, and to their use as raw materials and complete feed for animal feed. Background technique [0002] Compositions based on amino acids are used in a wide variety of fields, such as nutraceuticals, cosmetics, and human and animal nutrition, each of which has very different and specific applications. Mention may especially be made of the use for hair growth and shine, for improving sleep and reducing stress, and for the rebalancing of essential amino acids, and as a source of protein in infant nutrition and in dog and cat nutrition. [0003] One of the ways to obtain compositions based on amino acids is to produce hydrolysates of keratinous substances. [0004] Natural keratin substances mainly contain high molecular weight polypeptides with a highly cross-linked structure, which makes them di...

Claims

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Application Information

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IPC IPC(8): A23K10/26A23K20/142A23K20/147A23J1/10A23J3/34
CPCA23K10/26A23K20/142A23K20/147A23J1/10A23J3/341A23V2002/00A23V2250/06A23V2250/0626A23V2250/0654C07K1/12C07K1/14A23K10/20A23K50/40
Inventor 乔尔·迪佩雷雷诺·瑟尔格赫雷特
Owner 布列塔尼精细化工公司
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