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Novel antibacterial biological dye

A biological and dye technology, applied in the field of textile dyeing, can solve the problems of dyes losing antibacterial properties, easy hydrolysis, lack of affinity for cotton fibers, etc., and achieve the effect of good dyeing effect.

Pending Publication Date: 2020-09-04
杭州唯铂莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dye lacks affinity for cotton fibers, and because the quaternary ammonium salt in the molecule is combined with the dye parent through an amide bond, it is easily hydrolyzed, so that the dye loses its antibacterial properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The solid-cultured Photorhabdus Luminescens was expanded in LB liquid medium, and the medium ratio was 1.5% tryptone, 1.2% yeast extract, 0.8% NaCl, and the pH value was adjusted to 6, and 10% sodium glutamate was added , and cultured at 30°C for 20h. Use a low-speed centrifuge to separate the bacterial liquid and remove the bacterial cells. The liquid was subjected to high-speed centrifugation to obtain a solid, which was washed sequentially with methanol and ethyl acetate to obtain a dark blue substance.

Embodiment 2

[0015] The solid-cultured Streptomyces Lavendulae were expanded in LB liquid medium. The proportion of the medium was 2% tryptone, 1.8% yeast extract, and 1.2% NaCl, and the pH value was adjusted to 7, and 15% glutamine was added. Incubate at 37°C for 20h. Use a low-speed centrifuge to separate the bacterial liquid and remove the bacterial cells. The liquid was subjected to high-speed centrifugation to obtain a solid, which was washed sequentially with methanol and ethyl acetate to obtain a dark blue substance.

Embodiment 3

[0017] The solid-cultured Erwinia Chrysanthemi was expanded in LB liquid medium, and the medium ratio was 1.2% tryptone, 2% yeast extract, 1% NaCl, and the pH value was adjusted to 7, and 12% sodium glutamate was added , and cultured at 25°C for 20h. Use a low-speed centrifuge to separate the bacterial liquid and remove the bacterial cells. The liquid was subjected to high-speed centrifugation to obtain a solid, which was washed sequentially with methanol and ethyl acetate to obtain a dark blue substance.

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PUM

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Abstract

The invention discloses a novel antibacterial biological dye. The biological dye is prepared by culturing microorganisms, taking sodium glutamate or / and glutamine or / and an approximate compound as a substrate, and separating and purifying a culture to obtain a dark blue substance, namely, the biological dye, wherein the microorganisms are one or more of Photorhabdus Luminescens, Streptomyces Lavendulae and Erwinia Chrysanthemi, or engineering bacteria with the same effect; the dark blue substance is a bicyclo-3,3'-bipyridine molecule, and has typical carbonyl structure characteristics. The biological dye is cultured and separated from the microorganisms, has relatively high natural activity, is more environmental-friendly, and is insoluble in water; a water-soluble compound is formed underthe action of a reducing agent under an alkaline condition; fabric is dyed by dip dyeing or pad dyeing, and the like; and the biological dye has a good dyeing effect on natural fibers such as cottonfabric.

Description

technical field [0001] The invention belongs to the technical field of textile dyeing, in particular to a novel antibacterial biological dye. Background technique [0002] Textile dyeing and finishing are two important wet processing processes, while traditional textile dyeing and finishing are carried out separately, which has the disadvantages of high cost, high energy consumption and large amount of sewage discharge. The dyeing and finishing technology that combines dyeing and functional finishing in one bath is of great significance for reducing production costs, saving energy, reducing waste water discharge, and protecting the ecological environment. Therefore, many experts at home and abroad have carried out a lot of research in this field, and have achieved certain results in the research of dyeing, antistatic, flame retardant, anti-ultraviolet, shrink-proof and anti-wrinkle finishing in one bath. However, in most cases, due to the difference in dyeing and finishing ...

Claims

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Application Information

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IPC IPC(8): D06P1/00D06P3/60C12P39/00C12P17/16C12R1/56C12R1/18C12R1/01
CPCD06P1/00D06P3/60C12P39/00C12P17/165
Inventor 占纪勋张炜
Owner 杭州唯铂莱生物科技有限公司
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