Microecological compound premixed feed containing Pediococcus acidilactici and application thereof

A pre-mixed feed, Pediococcus lactis technology, applied in applications, microorganisms, animal feed, etc., can solve problems such as harm to human health, pathogenic bacteria resistance, etc., to reduce breeding diseases, superoxide dismutase and acid phosphatase activities. Improve and improve the effect of environmental water quality

Active Publication Date: 2020-09-18
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Adding antibiotics to feed is a traditional way to solve intestinal health problems of aquatic animals, but the use of antibiotics will lead to the development of pathogenic bacteria resistance and eventually endanger human health

Method used

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  • Microecological compound premixed feed containing Pediococcus acidilactici and application thereof
  • Microecological compound premixed feed containing Pediococcus acidilactici and application thereof
  • Microecological compound premixed feed containing Pediococcus acidilactici and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, screening and identification of strains

[0029] 1. Sample:

[0030] The intestines of live California perch were collected from a market in Jiaozhou City, Qingdao. Healthy and vigorous California perch intestines were extracted by aseptic operation for subsequent separation.

[0031] 2. Screening method

[0032] (1) Enrichment culture

[0033] Take the intestinal sample and put it into a grinder, put it into normal saline, homogenize it, and centrifuge at low speed, so that the bacteria in the sample are fully dispersed in the upper layer of normal saline, and then 10-fold gradient dilution. The above bacterial suspension was transferred to a liquid MRS medium with a 10% inoculum amount, and cultured at 30°C for 24h-48h to obtain an enriched bacterial suspension.

[0034] (2) Separation

[0035] Apply 0.2ml of bacterial suspension to DM solid medium plate by 10-fold dilution method, and cultivate in a 30°C incubator; when a single colony gr...

Embodiment 2

[0047] Application of embodiment 2 Pediococcus lactis MST02 in purifying aquaculture water

[0048] 1. Preparation of bacteria powder

[0049] Pediococcus lactis MST02 was expanded and cultivated to make 10 billion / g bacterial powder.

[0050] 2. Experimental location

[0051] A California perch breeding factory in Yancheng, Jiangsu.

[0052] 3. Experimental process

[0053] Six ponds with similar size and water quality were selected, three in each of the control group and the experimental group were paralleled, and the nitrite nitrogen content in each water body was detected respectively. Then add 10ppm of Pediococcus lactis powder to the water body of the experimental group, and do not add it to the control group. The water temperature in the experiment was 28-30°C, and the same equipment was used to continuously aerate the water body during the experiment. After 48 hours, the content of nitrite nitrogen in the water body was measured respectively, and the detection val...

Embodiment 3

[0058] Embodiment 3 Pediococcus lactis MST02 stomach acid resistance test

[0059] The simulated gastric juice components are: tryptone 8.5g, glucose 3.5g, sodium chloride 2.05g, calcium chloride 0.13g, potassium chloride 0.42g, potassium dihydrogen phosphate 0.55g, ox bile salt 0.05g, lysozyme 0.11g, Pepsin 13.5g, distilled water 1000ml, hydrochloric acid to adjust the pH to 1.5 and 2.5 respectively, and sterilize at 115°C for 20 minutes.

[0060] Another strain of Pediococcus lactis AS1.2696 was selected as the experimental control group from the Institute of Microbiology, Chinese Academy of Sciences.

[0061] Activate Pediococcus lactis MST02 and Pediococcus lactis AS1.2696 under the same conditions, then transfer it into 100ml MRS liquid medium according to the inoculum size of 0.6%, culture at 30°C for 24 hours, and add the two bacterial solutions to the prepared aseptic mock Gastric juice, after 0h and 2h, the number of viable bacteria was detected by plate counting met...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides microecological compound premixed feed and application thereof in aquaculture. The microecological compound premixed feed contains Pediococcus acidilactici MST02, and the collection number is CCTCCC NO: M2020904. The microecological compound premixed feed can significantlyimprove the intestinal health of aquatic animals, improve the immunity and digestion of aquatic animals, and improve the digestion and utilization rate of the feed and promote the growth of aquatic animals, can be widely used in the field of aquatic feed.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a microecological compound premixed feed containing Pediococcus lactis and an application thereof. Background technique [0002] The health of aquaculture animals is largely affected by their gut health, because the animal gut is the main place for digestion and absorption of nutrients, and gut health plays an important role in the healthy growth of aquatic animals. At this stage, the aquaculture industry pursues high efficiency, which is accompanied by high-density farming and the pursuit of growth speed, which requires that the farmed animals face a high load of feed nutrition, and the intestinal tract bears a heavy burden. Long-term high nutrient loads lead to intestinal mucosal damage in aquatic animals, further causing intestinal diseases. The high incidence of intestinal diseases in aquatic animals has become an important factor restricting the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K20/105A23K10/37A23K50/80A23K20/20A23K20/158A23K20/10C12N1/20C02F3/34C02F101/30C02F101/38C12R1/01
CPCA23K10/18A23K20/105A23K10/37A23K50/80A23K20/30A23K20/158A23K20/10C12N1/20C02F3/34C02F2101/30C02F2101/38Y02P60/87
Inventor 郭本月刘广倪梦丽魏万权周怡王磊
Owner 青岛玛斯特生物技术有限公司
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