Time-resolved optical biological detection equipment based on broadband stimulated radiation and detection imaging method thereof

Abstract

The invention discloses time-resolved optical biological detection equipment based on broadband stimulated radiation and a detection imaging method thereof. A pumping detection technology is combinedwith an optical coherence microscopy technology to realize stimulated radiation life imaging of a detected sample. The equipment comprises a super-continuum spectrum light source, a light modulation unit and an optical coherence microscopy unit, wherein the light modulation unit modulates input laser to generate pump light and probe light with time delay, the optical coherence microscopy unit is used for carrying out coherence detection on a biological sample, and the system analyzes a relation curve of signal intensity and time delay by using a phase vector method to obtain life-related information. The equipment can reflect a finer structure of an atomic energy level, has the advantages of long working distance, high temporal-spatial resolution, rapid imaging and the like, and has both tissue structure information and molecular specificity information. In addition, detection of non-fluorescence chromophores can be achieved, and detection deviation possibly caused by fluorescence labeling under specific conditions is avoided.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging, and in particular relates to a time-resolved optical biological detection device based on broadband stimulated radiation and a detection and imaging method thereof. Background technique [0002] Optical coherence tomography (OCT) or optical coherence microscopy (OCM) at a higher numerical aperture can realize in vivo imaging of living tissues with micron-level resolution and millimeter-level imaging depth, and has been used in the field of biomedicine. important role. However, since its contrast comes from the changes in the refractive index of cells and tissues, and the real part of the complex refractive index of tissues caused by cell metabolism does not change much under different physiological and pathological conditions, the OCT / OCM technology under the scattering contrast mechanism has a molecular specificity. However, there are deficiencies in the ability to identify ...

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Problems solved by technology

At the same time, the current detection of cell metabolism based on lifespan information is still mainly based on fluorescent chromophores, which come from exogenous fluorescent labels or autofluorescent substances, which also limits the application of these methods to a certain extent: First, A large number of substances containing metabolic information in cells are non-fluorescent chromophores, which cannot be imaged with fluorescent signals; secondly, fluorescent dyes are not suitable for labeling biological samples in some application environments, such as imaging living tissue cells, Fluorescent labels may affect the activity of the sample; finally, when observing some small metabolite molecules, since the size of these molecules is already smaller than that of fluorescent dye molecules, the introduction of fluorescent labels may also affect the resolution accuracy of the measurement

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