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Preventing and treating method for albinism of kelp

A treatment method, a technology for albinism, applied in horticultural methods, botanical equipment and methods, seaweed cultivation, etc., can solve problems such as affecting the supply of healthy kelp seedlings, breeding and production, and poor results.

Active Publication Date: 2020-09-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main symptom is that in the later stage of seedling cultivation, with the increase of light intensity, the tip of the kelp seedlings (tissues far away from the growth point of the base) will produce white spots. If it is not well controlled, green rot will occur from the diseased part. The rot disease spreads to the entire seedling leaves, which greatly affects the supply of healthy kelp seedlings and subsequent breeding production
[0003] Once albino seedlings are found in the kelp seedling stage, the main treatment method is to reduce the temperature, light intensity and increase the number of water changes in the seedling cultivation workshop to inhibit the reproduction of pathogenic bacteria and reduce the number of pathogenic bacteria, but the effect is not good. it is good
At present, there are no very effective measures and methods to prevent and control the outbreak of kelp albinism

Method used

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  • Preventing and treating method for albinism of kelp
  • Preventing and treating method for albinism of kelp
  • Preventing and treating method for albinism of kelp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: The effect of light on the growth of the pathogenic bacterium Pseudoalteromonas X-8

[0018] The applicant first conducted experiments on Pseudoalteromonas X-8, the causative bacterium of kelp albinism.

[0019] Among them, the pathogenic bacterium Pseudoalteromonas X-8 was selected from albino kelp seedlings in a kelp nursery in Rongcheng, Shandong in December 2017. The counterstaining experiment proved that this strain can cause healthy kelp seedlings to produce albinism.

[0020] Cultivate in Zobell 2216E medium, when X-8 reaches the exponential phase, use UV-6000 UV-Vis spectrophotometer (METASH) to measure the absorbance of bacterial growth at OD 600 (optical density at 600 nm). Purchase white light, blue light (400-450nm) and red light (620-760nm) two monochromatic LED light sources at Ningbo Jiangnan Instrument Factory. Set the light intensity of white light, blue light and red light to 40μmol·m -2 ·S -1 , 60μmol·m -2 ·S -1 ,, 160μmol·m -2 ·S -1 . Culture c...

Embodiment 2

[0021] Example 2: Cluster motility analysis

[0022] Under dark conditions, Pseudoalteromonas X-8 was cultured to the logarithmic growth phase at 25°C. Subsequently, the bacterial solution was centrifuged at 6000 g for 10 minutes at room temperature to obtain a cell pellet homogenate. Use an inoculating loop to inoculate the bacterial pellet onto the bacterial solid medium. Cultivate for 24 hours under blue, red, white and dark conditions at a temperature of 10±1°C. Three replicates are designed for each group of experiments. The light intensity is 80μmol·m -2 ·S -1 . It was found that at 80μmol·m -2 ·S -1 Under the conditions, white light and blue light inhibited the movement of Pseudoalteromonas X-8, while red light and darkness promoted the movement of Pseudoalteromonas X-8.

Embodiment 3

[0023] Example 3: Pathogenic bacteria-kelp counterstaining experiment

[0024] Take out the stored bacterial solution from the refrigerator at -20℃, pipette 1mL in 100mL Zobell 2216E liquid medium and culture in a shaking incubator. After 24 hours, pipette 1mL into 100mL Zobell 2216E liquid medium and continue culturing. After 12h, pipette 2mL overnight culture solution. Continue to culture in 200mL Zobell 2216E liquid medium, culture condition is 25℃, rotation speed is 120r / min. After 4h, draw the bacteria liquid to measure the concentration of the bacteria liquid; at this time, the growth of the bacteria liquid is in the logarithmic growth phase.

[0025] Select healthy and healthy kelp seedlings, place them in a petri dish filled with sterile seawater, and then cut the sporophytes into small pieces (1.0cm x 1.0cm) with a sterile blade. Put the kelp tissue block into the culture plate, and add 1mL Pseudoalteromonas X-8 bacterial solution (concentration 10 8 cfu / mL), cultured und...

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Abstract

The invention provides a preventing and treating method for albinism of kelp. According to the method, blue light or white light is used for inhibiting pathogenic bacteria of albinism of kelp; the illumination intensity of the white light is less than 40 [mu] mol.m <-2>. S <-1> or more than 160 [mu] mol.m <-2>. S <-1>, and the illumination intensity of the blue light is not lower than 40 mu [mu] mol.m <-2>. S <-1> The kelp albino disease pathogenic bacterium is a Pseudoalteromonas X-8 strain, and the preservation number of the kelp albino disease pathogenic bacterium is CGMCC 20099. The methodprovided by the invention can provide a theoretical basis for kelp seedling enterprises to formulate correct and proper illumination intensity management, and provide illumination intensity parameters for screening albinism-resistant kelp strains.

Description

Technical field [0001] The invention belongs to the technical field of seaweed disease prevention and treatment, and specifically relates to a method for preventing and treating kelp albinism. Background technique [0002] Kelp belongs to the genus Laminaria of Phaeophyta. It is a large brown algae and a large perennial edible algae. Shandong, Fujian and Liaoning are the main producing areas of kelp in my country, accounting for more than 90% of the country's total production. Like crops, kelp, a cash crop, will suffer serious diseases during the nursery stage. In recent years, albinism is a disease that often occurs in the late stage of kelp nursery (one and a half months or two months after the nursery starts). The main symptom is that in the late stage of seedling cultivation, with the increase of light intensity, white patches will appear on the tip of kelp seedlings (tissues far away from the base growth point). If not well controlled, green and green spots will occur from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00A01G13/00A01G7/04
CPCA01G33/00A01G13/00A01G7/045
Inventor 王高歌韩琦张晓阳
Owner OCEAN UNIV OF CHINA
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