Sample treatment and detection integrated reagent cup and sample detection method

Abstract

The invention discloses a sample treatment and detection integrated reagent cup with an active material transfer function. The reagent cup comprises a rotary cover, a reagent cup body and an injectionpipettor, wherein one end of the reagent cup body is connected with the rotary cover, and the other end of the reagent cup body is connected with a kit; and the injection pipettor penetrates throughthe rotary cover and the reagent cup body, and the bottom of the injection pipettor is far away from or close to the kit through movement. According to the reagent cup, a to-be-detected sample in thekit is sucked through the injection pipettor and evenly mixed with various reaction reagents, then a detection reaction is carried out, and a detection result is obtained; the whole process is fully automatically carried out in the reagent cup; a limiting sealing structure of the cup and an isolation sealing film of a reagent ensure that a product is detected in a closed environment, so that aerosol pollution is avoided, and the sealing performance is good; the to-be-detected sample enters the kit, and the detection result is directly obtained through control of a matched detection instrument,so that the automation degree is high; the structure is simple, the cost is low, and the detection accuracy is high; and meanwhile, sample nucleic acid can be extracted through magnetic particles.

Description

technical field [0001] The invention belongs to the technical field of medical equipment, and in particular relates to a sample processing and detection integrated reagent cup and a test sample method with an active material transfer function. Background technique [0002] Molecular detection technology platforms mainly include polymerase chain reaction technology, molecular hybridization technology, DNA sequencing technology, gene chips, etc. Molecular detection technology has been widely used in the diagnosis of infectious diseases, investigation of epidemics, food hygiene inspection, early diagnosis of tumors and genetic diseases, forensic identification, individualized diagnosis and treatment of tumors, blood screening, prenatal screening, pharmacogenomics research in various fields. It is the most rapidly developing clinical testing technology at present. [0003] Polymerase chain reaction technology (PCR) is a molecular biology technique used to amplify specific DNA ...

AI Technical Summary

Problems solved by technology

[0007] 1) There are many experimental steps;

[0008] 2) Low degree of automation;

[0009] 3) A specialized PCR laboratory is required;

[0010] 4) High requirements for operators;

[0011] 5) Easy aerosol pollution

[0015] 3) PCR tube mold opening requirements are high and the cost is high;

[0016] 4) The process of puncturing the sealing film is risky

[0017] Saipei Company GeneXpert has applied for several related patents, including U.S. Patent Document (US9669409) disclosing a kind of lid structure, the structure and function of the patent US9669409 base, the PCR reaction tube structure disclosed among the patent US9322052; The patent applied by GeneXpert does not involve the function of magnetic particle extraction of samples, and magnetic particle extraction is a commonly used method; the reaction tube must be manually inserted into the reaction cup, which is cumbersome to operate; only one reaction tube can be made at a time, and the throughput is limited

[0018] Chinese patent document (Application No.: 2018102991469) discloses a sample processing and detection reagent cup box provided with a material transfer structure, including a box body formed by an outer cover, and the top outer cover of the box body is provided with an openable sample inlet, so The inner side of the box body is provided with a loading disc, and the middle part of the loading disc is provided with a round hole, the side wall of the round hole is provided with a second fixing buckle, and the periphery of the round hole is provided with a waste pad set on the loading disc. The liquid storage bin, the first card slot, the second card slot and the sealing hole, and the waste liquid storage bin, the first card slot and the second card slot all form a sealing area through the sealing film, and a material transfer structure is arranged in the circular hole , the plastic tube for sample processing is glued and sealed in the first card slot; however, in this technical solution, the PCR tube is integrated into other modules and molded together, which requires high production technology and high cost, and the seal is pierced with a pipette tip film at risk

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