Immunohistochemical kit for rapidly identifying lung cancer and sclerotic lung cytoma during operation

Abstract

The invention relates to an immunohistochemical kit for rapidly identifying a lung cancer and sclerotic lung cytoma in an operation. The kit comprises a frozen stationary liquid, a peroxidase sealingagent, a CK wide (AE1 / AE3) antibody, a P40 antibody, a TTF1 antibody, a VIM antibody, an enzyme-labeled goat anti-mouse / rabbit polymer, a DAB chromogenic substrate, a DAB chromogenic buffer solution,a hematoxylin redyeing solution and a cleaning buffer solution, and the invention also provides a use method of the immunohistochemical detection kit. According to the kit, quick immunohistochemical staining is carried out on frozen sections in the operation; CK wide, VIM, TTF1 and p40 detection indexes are used for assisting in distinguishing the sclerotic lung cytoma and the lung cancer; and anintraoperative pathological judgment basis is increased from single morphological HE staining to combination of immunohistochemistry and morphology, and a comprehensive and reliable diagnosis basis isprovided for pathologists so that diagnosis accuracy is improved, and a misdiagnosis rate and a missed diagnosis rate are reduced.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an immunohistochemical kit for quickly distinguishing lung cancer and sclerosing pneumocytoma during operation. Background technique [0002] At present, the main method of intraoperative biopsy is HE staining of frozen section during operation. Frozen section is fast frozen section during operation. Tissue sections are made, after fast hematoxylin and eosin staining, the pathologist observes under the microscope immediately, and makes a judgment in a short time. The whole process generally needs to be completed within half an hour, while the paraffin sections are fixed in neutral buffered formalin, Paraffin embedding generally takes 2 to 3 days to complete. However, because the judgment of HE staining only depends on morphological evidence, the tissue will inevitably form crystals during the freezing process, and the quality of cell morphology is poor, resulting in cell d...

AI Technical Summary

Problems solved by technology

However, because the judgment of HE staining only depends on morphological evidence, the tissue will inevitably form crystals during the freezing process, and the quality of cell morphology is poor, resulting in cell deformation and artificial artifacts. In addition, limited materials for frozen sections increase the difficulty of diagnosis. , there is a possibility of misdiagnosis

In the intraoperative frozen diagnosis of lung tumors, sclerosing pneumocytoma has various histological forms, and it is easy to be misdiagnosed as cancer in clinical and imaging studies. The accuracy rate of intraoperative frozen diagnosis of sclerosing pneumocytoma is as low as 37.7%.

[0003] Whether the frozen diagnosis is correct or not is very important to the location, tissue size and quantity of the clinician and the pathologist. A frozen section made by conventional sampling methods often cannot reflect the whole picture of the tumor

When multiple forms are mixed together, it is more likely to be confused with cancer, and its intraoperative differential diagnosis is difficult, which can easily lead to misdiagnosis, missed diagnosis, and overdiagnosis, which will burden patients physically and mentally.

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