Purification method of cannabidiol

Abstract

The invention provides a purification method of cannabidiol. The purification method comprises the following steps: removing a large amount of impurities from an extract of an industrial cannabidiol crude extract by a solvent method, and directly crystallizing and re-crystallizing to prepare high-purity cannabidiol. The purification method does not need expensive chromatography equipment, large heat supply equipment or concentration equipment, and the problem that the cannabidiol production investment is large is solved. Because a large amount of solvent and heat energy is not consumed in theproduction process, compared with a chromatography process, the production cost is greatly reduced.

Description

technical field

[0001] The invention relates to a method for purifying cannabidiol, in particular to a method for purifying cannabidiol with industrial hemp extract, and belongs to the field of biochemical industry. Background technique

[0002] Cannabidiol is a non-psychoactive ingredient extracted from industrial hemp, which does not cause physiological dependence, has good tolerance and high safety, and is effective for neurological diseases, epilepsy, tumors, depression, Parkinson's, and Alzheimer's It has shown good therapeutic properties in many diseases, such as inflammation, pain and so on. The application of cannabidiol in the field of medicine will have broad market prospects.

[0003] At present, there are many methods for extracting cannabidiol concentrates, but the separation and purification of cannabidiol from crude extracts basically adopts column chromatography. This method mainly has the following disadvantages: column chromatography equipment is expensive...

Examples

Embodiment 1

[0023] Take 100g of ethanol crude extract of industrial hemp with 16.2% cannabidiol content, heat and reflux with 300ml of ethanol at 80°C for 20 minutes, after dissolving, add 3200ml of sulfuric acid aqueous solution with pH=4, stir at 120r / min for 10 minutes, let stand After 8 hours, divide the dehydration solution to obtain 510ml of precipitation solution; add 3200ml of sodium hydroxide aqueous solution with pH=10 to the above precipitation solution, stir at 120r / min for 10 minutes, let stand for 12 hours, and divide the dehydration solution to obtain 480ml of precipitation solution ; Add 3800ml of purified water to the above precipitation solution, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter the dehydrated solution, and obtain 134g of aqueous precipitation; add 550ml of absolute ethanol to the above precipitation, heat at 70°C and 120r / min Stir for 15 minutes, after dissolving, let stand at 5°C for 6 hours, remove the insoluble matter by filtration, s...

Embodiment 2

[0025] Take 100g of methanol crude extract of industrial hemp with 16.5% cannabidiol content, heat and reflux with 400ml of methanol at 70°C for 30 minutes, after dissolving, add 5000ml of nitric acid aqueous solution with pH=4.5, stir at 120r / min for 10 minutes, let stand After 10 hours, divide the dehydration solution to obtain 490ml of precipitation solution; add 3000ml of potassium hydroxide aqueous solution with pH=11 to the above precipitation solution, stir at 120r / min for 15 minutes, let stand for 12 hours, and divide the dehydration solution to obtain 450ml of precipitation solution ; Add 3500ml of purified water to the above precipitate, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter or centrifuge to separate the dehydrated liquid, and get 105g of aqueous precipitate; add 400ml of methanol to the above precipitate, heat at 70°C and 120r / min Stir for 10 minutes, after dissolving, let it stand at 0°C for 4 hours, filter or centrifuge to remove insolu...

Embodiment 3

[0027] Take 100g of 6# solvent oil crude extract of industrial hemp with a cannabidiol content of 15.8%, heat and reflux with 500ml acetone at 60°C for 30 minutes, after dissolving, add 5500ml of nitric acid aqueous solution with pH=3.5, and stir at 120r / min for 10 minutes , stand still for 12 hours, divide the dehydration liquid to obtain 485ml of precipitation solution; add 3500ml of lithium hydroxide aqueous solution with pH=10 to the above precipitation solution, stir at 120r / min for 15 minutes, let stand for 12 hours, and divide the dehydration liquid to obtain 430ml of precipitation solution; add 4500ml of purified water to the above precipitation solution, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter the dehydrated solution, and obtain 103g of aqueous precipitation; add 500ml of acetone to the above precipitation, heat at 60°C and 120r / min Min and stirred for 10 minutes, after dissolving, stand at 10°C for 10 hours, filter to remove insoluble matter...

technical field

[0001] The invention relates to a method for purifying cannabidiol, in particular to a method for purifying cannabidiol with industrial hemp extract, and belongs to the field of biochemical industry. Background technique

[0002] Cannabidiol is a non-psychoactive ingredient extracted from industrial hemp, which does not cause physiological dependence, has good tolerance and high safety, and is effective for neurological diseases, epilepsy, tumors, depression, Parkinson's, and Alzheimer's It has shown good therapeutic properties in many diseases, such as inflammation, pain and so on. The application of cannabidiol in the field of medicine will have broad market prospects.

[0003] At present, there are many methods for extracting cannabidiol concentrates, but the separation and purification of cannabidiol from crude extracts basically adopts column chromatography. This method mainly has the following disadvantages: column chromatography equipment is expensive...

Examples

Embodiment 1

[0023] Take 100g of ethanol crude extract of industrial hemp with 16.2% cannabidiol content, heat and reflux with 300ml of ethanol at 80°C for 20 minutes, after dissolving, add 3200ml of sulfuric acid aqueous solution with pH=4, stir at 120r / min for 10 minutes, let stand After 8 hours, divide the dehydration solution to obtain 510ml of precipitation solution; add 3200ml of sodium hydroxide aqueous solution with pH=10 to the above precipitation solution, stir at 120r / min for 10 minutes, let stand for 12 hours, and divide the dehydration solution to obtain 480ml of precipitation solution ; Add 3800ml of purified water to the above precipitation solution, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter the dehydrated solution, and obtain 134g of aqueous precipitation; add 550ml of absolute ethanol to the above precipitation, heat at 70°C and 120r / min Stir for 15 minutes, after dissolving, let stand at 5°C for 6 hours, remove the insoluble matter by filtration, s...

Embodiment 2

[0025] Take 100g of methanol crude extract of industrial hemp with 16.5% cannabidiol content, heat and reflux with 400ml of methanol at 70°C for 30 minutes, after dissolving, add 5000ml of nitric acid aqueous solution with pH=4.5, stir at 120r / min for 10 minutes, let stand After 10 hours, divide the dehydration solution to obtain 490ml of precipitation solution; add 3000ml of potassium hydroxide aqueous solution with pH=11 to the above precipitation solution, stir at 120r / min for 15 minutes, let stand for 12 hours, and divide the dehydration solution to obtain 450ml of precipitation solution ; Add 3500ml of purified water to the above precipitate, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter or centrifuge to separate the dehydrated liquid, and get 105g of aqueous precipitate; add 400ml of methanol to the above precipitate, heat at 70°C and 120r / min Stir for 10 minutes, after dissolving, let it stand at 0°C for 4 hours, filter or centrifuge to remove insolu...

Embodiment 3

[0027] Take 100g of 6# solvent oil crude extract of industrial hemp with a cannabidiol content of 15.8%, heat and reflux with 500ml acetone at 60°C for 30 minutes, after dissolving, add 5500ml of nitric acid aqueous solution with pH=3.5, and stir at 120r / min for 10 minutes , stand still for 12 hours, divide the dehydration liquid to obtain 485ml of precipitation solution; add 3500ml of lithium hydroxide aqueous solution with pH=10 to the above precipitation solution, stir at 120r / min for 15 minutes, let stand for 12 hours, and divide the dehydration liquid to obtain 430ml of precipitation solution; add 4500ml of purified water to the above precipitation solution, stir at 120r / min for 10 minutes, let it stand for 12 hours, filter the dehydrated solution, and obtain 103g of aqueous precipitation; add 500ml of acetone to the above precipitation, heat at 60°C and 120r / min Min and stirred for 10 minutes, after dissolving, stand at 10°C for 10 hours, filter to remove insoluble matter...

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