Cytochalasin derived from phenylalanine and preparation method and application thereof
A technology of cytochalasin and phenylalanine, applied in the field of cytochalasin and its preparation
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Embodiment 1
[0032] The present invention will be further described in conjunction with embodiment, accompanying drawing:
[0033] making process
[0034] Inoculate the endophytic fungus Xylaria sp.CFL5 on the leaves of Cephalotaxus fortunei Hook.f in YMG medium (composed of: yeast extract 4g, malt extract 10g, glucose 10g, distilled water 1L), culture at 140rpm, 28°C After 5 days, the seed solution was obtained. Afterwards, it was added to rice culture medium (80 g rice, 4 g sucrose, 120 mL distilled water) according to the inoculum amount of 10 mL per bottle, and cultivated at a constant temperature of 28 ° C for 3 weeks. When the fermentation is over, chop the rice culture medium. EtOAc was extracted at room temperature for 5 times, and the solvent was distilled off under reduced pressure to obtain a crude extract. The crude extract was segmented using a macroporous resin, and 30%, 50%, 80%, 95% EtOH was used as the gradient elution of the mobile phase to obtain 4 sample segments (Fr...
Embodiment 2
[0037] Inoculate the endophytic fungus Xylaria sp.CFL5 on the leaves of Cephalotaxus fortunei Hook.f in YMG medium (composed of: 40g yeast extract, 100g malt extract, 100g glucose, 10L distilled water), culture at 140rpm, 30°C After 5 days, the seed solution was obtained. Then it was added into rice culture medium (800g rice, 40g sucrose, 1200mL distilled water) according to the inoculum amount of 100mL per bottle, and cultivated at a constant temperature of 28°C for 3 weeks. When the fermentation is over, chop the rice culture medium. EtOAc was extracted at room temperature for 5 times, and the solvent was distilled off under reduced pressure to obtain a crude extract. The crude extract was segmented using a macroporous resin, and 30%, 50%, 80%, 95% EtOH was used as the gradient elution of the mobile phase to obtain 4 sample segments (Fr.1–4).
[0038] Fr.3 Use normal phase silica gel column for segmentation, mobile phase is petroleum ether / ethyl acetate mixed solvent gradi...
Embodiment 3
[0040] Inoculate the endophytic fungus Xylaria sp.CFL5 on the leaves of Cephalotaxus fortunei Hook.f in YMG medium (composed of: 40g yeast extract, 100g malt extract, 100g glucose, 10L distilled water), culture at 140rpm, 30°C After 5 days, the seed solution was obtained. Then it was added into rice culture medium (800g rice, 40g sucrose, 1200mL distilled water) according to the inoculation amount of 100mL per bottle, and cultivated at a constant temperature of 28°C for 2 weeks. When the fermentation is over, chop the rice culture medium. EtOAc was extracted at room temperature for 5 times, and the solvent was distilled off under reduced pressure to obtain a crude extract. The crude extract was segmented using a macroporous resin, and 30%, 50%, 80%, 95% EtOH was used as the gradient elution of the mobile phase to obtain 4 sample segments (Fr.1–4).
[0041] Fr.3 Use normal phase silica gel column for segmentation, mobile phase is petroleum ether / ethyl acetate mixed solvent gr...
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