Method for extracting elastin peptide from bovine cartilage

Abstract

The invention discloses a method for extracting elastin peptide from bovine cartilage. The method comprises the following steps: grinding the bovine cartilage into powder; transferring the powder intoan extraction tank, adding purified water which is 3-4 times of the weight of the powder, heating a mixture to 95 DEG C, and performing stirring for 2 hours; cooling to 50-55 DEG C, adding compound protease, and after the reaction is finished, carrying out enzyme deactivation to obtain an enzyme shearing solution; cooling to 50-60 DEG C, and carrying out decolorization and deodorization treatment; separating a solute from a solvent by adopting a permeable membrane; separating organic small molecules with a molecular weight of 150-1000 Dal by adopting a nanofiltration membrane; and performingconcentration and spray drying. Protein peptides and non-peptide substances are different in thermal stability, the bovine cartilage powder is subjected to high-temperature extraction, an extracting solution is sheared through compound protease and then concentrated and filtered through different permeable membranes, small peptides with the molecular weight of 150-1000 Dal are obtained, the peptides with the molecular weight range account for 85% or above of peptides, and the absorption efficiency can be greatly improved.

Description

technical field

[0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting elastin peptide from bovine cartilage. Background technique

[0002] Two forms of elastin have been found: elastin I is found in the nuchal ligament, aorta and skin, and elastin II is obtained from cartilage.

[0003] Elastin is the main component of elastic fibers in hide tissue. The peptide chain of elastin contains more than 713 amino acid residues. Unlike collagen and keratin, there is no continuous repeating periodic structure throughout the entire peptide chain in the amino acid sequence of elastin, but there are alternating hydrophobic and hydrophilic peptide segments. Desmosin and desmosin formed by oxidizing lysyl are unique cross-linking structures of elastin. These cross-linked structural lattices combine more than two team dimensions. Elastic fibers are mainly found in ligaments and vessel walls. Coexistence of elastic fibers...

Examples

Embodiment 1

[0024] Such as figure 1 Shown a kind of method extracting elastin peptide from bovine cartilage, comprises the following steps:

[0025] A, the bovine cartilage is ground into powder;

[0026] B. Transfer the powder to the extraction tank, add pure water 3-4 times the weight of the powder, raise the temperature to 95°C, stir and continue for 2 hours;

[0027] C. Lower the temperature to 50°C-55°C, adjust to the optimal reaction conditions of the enzyme, add the compound protease, and inactivate the enzyme after the reaction to obtain the enzyme cutting solution; specifically, the method of inactivating the enzyme can be carried out by heating. The added amount of the compound protease is 0.2% by weight of bovine cartilage powder, so as to ensure the saturation of the compound protease, and the compound protease will not be excessively wasted.

[0028] D. Cool down to 50-60°C, and carry out decolorization and deodorization treatment; this step preferably uses a plate-and-fram...

Embodiment 2

[0033] Based on the principles of the above embodiments, this embodiment discloses a specific embodiment, specifically:

[0034] Pre-treatment: Wash the bovine cartilage, refine it and pulverize it.

[0035] Add 1kg bovine cartilage powder and 4kg hot water in the extraction tank, keep the water temperature at 95°C, and extract for 2 hours.

[0036] Lower the temperature to 55°C, add 0.2% bovine cartilage powder compound protease, and control the water temperature at 50°C-55°C for enzymatic hydrolysis. After the enzymatic hydrolysis, the temperature was raised to 95°C and kept for 30 minutes.

[0037] Cool down to 50-60°C for deodorization and decolorization treatment; pre-coat the plate and frame filter with diatomaceous earth, add 30g of activated carbon and mix and stir for 0.5h, then pressurize and pass through the plate and frame filter until the filtrate is yellow, clear and transparent liquid .

[0038] Composite tubular membrane and other permeable membranes are use...

technical field

[0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting elastin peptide from bovine cartilage. Background technique

[0002] Two forms of elastin have been found: elastin I is found in the nuchal ligament, aorta and skin, and elastin II is obtained from cartilage.

[0003] Elastin is the main component of elastic fibers in hide tissue. The peptide chain of elastin contains more than 713 amino acid residues. Unlike collagen and keratin, there is no continuous repeating periodic structure throughout the entire peptide chain in the amino acid sequence of elastin, but there are alternating hydrophobic and hydrophilic peptide segments. Desmosin and desmosin formed by oxidizing lysyl are unique cross-linking structures of elastin. These cross-linked structural lattices combine more than two team dimensions. Elastic fibers are mainly found in ligaments and vessel walls. Coexistence of elastic fibers...

Examples

Embodiment 1

[0024] Such as figure 1 Shown a kind of method extracting elastin peptide from bovine cartilage, comprises the following steps:

[0025] A, the bovine cartilage is ground into powder;

[0026] B. Transfer the powder to the extraction tank, add pure water 3-4 times the weight of the powder, raise the temperature to 95°C, stir and continue for 2 hours;

[0027] C. Lower the temperature to 50°C-55°C, adjust to the optimal reaction conditions of the enzyme, add the compound protease, and inactivate the enzyme after the reaction to obtain the enzyme cutting solution; specifically, the method of inactivating the enzyme can be carried out by heating. The added amount of the compound protease is 0.2% by weight of bovine cartilage powder, so as to ensure the saturation of the compound protease, and the compound protease will not be excessively wasted.

[0028] D. Cool down to 50-60°C, and carry out decolorization and deodorization treatment; this step preferably uses a plate-and-fram...

Embodiment 2

[0033] Based on the principles of the above embodiments, this embodiment discloses a specific embodiment, specifically:

[0034] Pre-treatment: Wash the bovine cartilage, refine it and pulverize it.

[0035] Add 1kg bovine cartilage powder and 4kg hot water in the extraction tank, keep the water temperature at 95°C, and extract for 2 hours.

[0036] Lower the temperature to 55°C, add 0.2% bovine cartilage powder compound protease, and control the water temperature at 50°C-55°C for enzymatic hydrolysis. After the enzymatic hydrolysis, the temperature was raised to 95°C and kept for 30 minutes.

[0037] Cool down to 50-60°C for deodorization and decolorization treatment; pre-coat the plate and frame filter with diatomaceous earth, add 30g of activated carbon and mix and stir for 0.5h, then pressurize and pass through the plate and frame filter until the filtrate is yellow, clear and transparent liquid .

[0038] Composite tubular membrane and other permeable membranes are use...

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