35 results about "Atpase reaction" patented technology

The present invention provides an integrated microfluidic electrospray chip system and analytical method thereof, characterized in which the proteinase reaction, solid-phase extraction mechanism, electrophoresis and mass spectrometry are integrated into one system. This system allows continuous, fast and on-line detection and identification of a sample, where the sample is firstly hydrolyzed and desalted, and then introduced into the microfluidic electrospray chip to undergo electrophoresis. Finally, the separated sample is introduced into a mass spectrometer by means of electrospray for continuous detection and identification. This system applies mainly in the identification of biochemical substances, such as proteins.

The present invention relates to a method of producing keratin hydrolysate comprising the steps of: i) reacting keratin material with a protease; and ii) reacting keratin material with a chemical oxidant; wherein step ii occurs: a) after step i); b) during step i) when the selected protease hydrolyses under the pH conditions used for the chemical reaction and / or c) prior to step i) when the selected protease hydrolyses under the reaction conditions used for the chemical reaction; keratin hydrolysate so produced and uses thereof.

Stabilizers for colorants used for detection of enzymatic reactions, having a cyclodextrin and a catalase; reagents including stabilizers; and methods of use of such stabilizers and reagents.

The present invention provides a protein comprising an amino acid sequence in which arginine at position 61 of a protein comprising the amino acid sequence represented by SEQ ID NO: 1 is substituted to an amino acid selected from the group consisting of glycine, alanine, valine, leucine, serine, threonine, proline, cysteine, methionine, asparagine, glutamine, and aspartic acid; and a method for measuring a glycated hemoglobin in a sample, wherein the method comprises reacting a glycated hemoglobin in a sample with a protease to produce a glycated hexapeptide, then reacting the produced glycated hexapeptide with the aforementioned protein, and measuring a substance produced or consumed by the reaction.

An HGF precursor protein variant, in which a peptide structure comprises a sequence including a peptide chain X inserted between an α chain of HGF or a polypeptide where 1 to 20 amino-acid residues from the C-terminus of the α chain are deleted, and a β chain of HGF or a polypeptide where 1 to 20 amino-acid residues from the N-terminus of the β chain are deleted; wherein (i) the peptide chain X has an amino-acid sequence of at least two residues, (ii) the peptide chain X can be cleaved by a protease reaction or a chemical reaction, and (iii) a protein obtained by cleaving at least one site of the peptide chain X has HGF action.

The invention discloses a histological method for distinguishing three kinds of carp muscle cells, wherein the method comprises the specific steps: freezing a treated sample with isoamyl and liquid nitrogen; freezing the sample at low temperature, slicing, and drying at room temperature; putting the slice into a constant-temperature pretreatment liquid, treating, and then cleaning with a cleaningliquid; adding a cleaning liquid into the slice, pouring out after the temperature is constant, adding an ATPase reaction liquid with the same temperature, carrying out a reaction, and cleaning and impregnating with distilled water; soaking the slice with a CaCl2 solution, a CoCl2 solution and an ammonium sulfide solution successively, and cleaning and impregnating with distilled water after the soaking is finished; absorbing excess moisture of the slice, covering with cover glass, sealing the slice, observing with an optical microscope, and thus distinguishing the three kinds of carp muscle cells. Boundary lines of red meat cells, pink meat cells and white meat cells in microscope images obtained by the distinguishing method are obvious, and the distribution of the three kinds of carp muscle cells can be clearly distinguished.

The invention relates to a pharmaceutical reagent, in particular to a reagent for determining the percentage of glycosylated hemoglobin. The reagent consists of the following substances: a dissolution buffering agent which is used for releasing hemoglobin from red blood cells in a blood sample, protease which can cut off a glycosylated valine or glycosylated polypeptide fragment of an N-terminal end of a beta chain of the glycosylated hemoglobin, an oxidant which can oxidize and eliminate interfering substances in the sample, an oxidant which can denature the hemoglobin and can accelerate the reaction rate of the protease, a reaction accelerator which can accelerate the reaction rate of the protease, fructosyl-amino acid oxidase undergoing oxidation-reduction reaction with the protease which can cut off the glycosylated valine or glycosylated polypeptide fragment of the N-terminal end of the beta chain of the glycosylated hemoglobin in the substance a, a color development agent which can undergo color development reaction with generated hydrogen peroxide, and peroxidase which can catalyze the hydrogen peroxide and the color development agent for the color development reaction. Compared with the prior art, the invention has the advantage that the reagent can directly determine the glycosylated hemoglobin.

The invention provides a sunflower disc extract and its use in treating constipation. The preparation process of the sunflower disc extract is to soak the powdered sunflower disc in water for a certain period of time, then heat it to 45-55°C, add cellulase to react 1- After 4 hours, add proteolytic enzymes (alkaline protease and neutral protease) to react for 1-2 hours, and finally add flavor protease to react for 1-2 hours, filter and concentrate while hot, and dry into powder or granules. The extraction rate of small molecule peptides and polysaccharides in the sunflower disk extract is greatly increased. The sunflower disk extract can treat constipation, and it has been verified through animal experiments and crowd trials, and the results show that the invention can significantly promote defecation of patients without toxic and side effects.

Provided are fish meal proteolytic products, its preparation process and application, wherein the preparing process comprises, charging deionized water into fish meal, stirring in a container, charging ammonia to adjust the pH of the solution, charging basified protease for reaction, then charging ammonia to maintain the pH of the reaction system, water-bathing the reaction solution, removing enzyme, centrifuging, freeze drying the supernatant fluid into dried powder, finally freezing for later use.