Waste bacteria treatment method and waste bacteria recycling method in waste bacterial production

A treatment method, the technology of spiramycin, is applied in the direction of microorganism-based methods, biochemical equipment and methods, and the dissolution of microorganisms, which can solve the problem of increased burden on enterprises, secondary environmental pollution, environmental cycles of antibiotics or analogues that cannot be ruled out, etc. problems, to achieve the effect of avoiding outflow and pollution

Active Publication Date: 2018-07-13
TOPFOND PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The limitation of high-temperature incineration lies in the high equipment investment and high operating costs, which will greatly increase the burden on enterprises. If the incineration is not complete, it...

Method used

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  • Waste bacteria treatment method and waste bacteria recycling method in waste bacterial production
  • Waste bacteria treatment method and waste bacteria recycling method in waste bacterial production
  • Waste bacteria treatment method and waste bacteria recycling method in waste bacterial production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 50 L of the fermented spiramycin production broth after fermentation, adjust the pH to 5.5 with hydrochloric acid, and filter using the ceramic membrane filtration system of Nanjing Jiuwu Company. The diameter of the ceramic membrane filter element is 25 nm. After filtration, 35 L of concentrated solution intercepted by the ceramic membrane was obtained.

[0030] Transfer the concentrate to the reaction kettle, heat up to 35°C, add lysozyme (Zhejiang Aijiesi Biotechnology Co., Ltd.) 4 × 10 7 U, reacted for 3 hours; the temperature of the reaction solution was raised to 55°C, and the unit enzyme activity was added to be 5×10 4 Alkaline protease 20g (Shandong Sukehan Biological Engineering Co., Ltd.) of U / g reacted for 6 hours; Advance into spray drying (Shanghai Dacheng Experimental Equipment Co., Ltd.) to obtain waste bacteria powder 4.2kg, wherein moisture 11.5%.

[0031] Use the obtained waste bacteria powder to replace 75% of the total amount of soybean cake po...

Embodiment 2

[0035] Take 50L of the fermented spiramycin production broth that has finished fermentation, adjust the pH to 4.9 with hydrochloric acid, and filter using the ceramic membrane filtration system of Nanjing Jiuwu Company. The diameter of the ceramic membrane filter element is 50nm. After filtration, 32L of concentrated solution was obtained.

[0036] Transfer the concentrate to the reaction kettle, heat up to 30°C, add lysozyme (Zhejiang Aijiesi Biotechnology Co., Ltd.) 4 × 10 7 U, reacted for 5 hours; the temperature of the reaction solution was raised to 65°C, and the unit enzyme activity was added to be 5×10 4 Alkaline protease 20g (Shandong Sukehan Biological Engineering Co., Ltd.) of U / g was reacted for 8 hours; Advance into spray drying (Shanghai Dacheng Experimental Equipment Co., Ltd.) to obtain waste bacteria powder 3.9kg, moisture 10.7%.

[0037] The obtained waste bacteria powder was used to replace the soybean cake powder in the original fermentation base material i...

Embodiment 3

[0041] Take 50L of the fermented spiramycin production broth after fermentation, adjust the pH to 6.5 with hydrochloric acid, and filter using the ceramic membrane filtration system of Nanjing Jiuwu Company. The diameter of the ceramic membrane filter element is 25nm. After filtration, 34L of concentrated solution was obtained.

[0042] Transfer the concentrate to the reaction kettle, heat up to 35°C, add lysozyme (Zhejiang Aijiesi Biotechnology Co., Ltd.) 4 × 10 7 U, reacted for 4 hours; the reaction solution was warming up to 60°C, added 20g of neutral protease (Shandong Sukehan Biological Engineering Co., Ltd.), and reacted for 6 hours; into spray drying (Shanghai Dacheng Experimental Equipment Co., Ltd.) to obtain waste Bacteria powder 3.8kg, moisture 10.3%.

[0043] The obtained waste bacteria powder was used to replace the soybean cake powder in the original fermentation base material in a proportion of 100% for shake-flask fermentation, and the original fermentation ra...

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Abstract

The invention discloses a waste bacteria treatment method and a waste bacteria recycling method in waste bacterial production. The waste bacteria treatment method comprises: a, adjusting the pH valueof a spiromycin production fermentation broth to 4.0-6.5, and filtering by using a ceramic membrane to obtain a concentrated liquid retained by the ceramic membrane; b, adding lysozyme to the concentrated liquid to obtain a lysozyme reaction solution; c, adding protease to the lysozyme reaction solution to obtain a protease reaction solution; and d, carrying out spray drying on the protease reaction solution to obtain waste bacteria powder. The waste bacteria recycling method comprises: replacing the organic nitrogen source in fermentation production of spiramycin with the obtained waste bacteria powder. With the methods of the present invention, the waste bacteria can achieve the fermentation raw material condition, and can be reused for the production of the original product so as to avoid the external flow of the waste bacteria.

Description

technical field [0001] The invention relates to the field of antibiotic fermentation production, in particular to a method for treating waste cells and a method for recycling waste cells in the fermentation production of spiramycin. Background technique [0002] my country is a big producer of antibiotics, and a large amount of antibiotic waste residue is produced every year, which seriously affects my country's environmental protection and the development process of the antibiotic fermentation industry. In 2002, the No. 176 document of the Ministry of Agriculture of the People's Republic of China included antibiotic dregs in the "Catalogue of Drugs Prohibited for Use in Feed and Animal Drinking Water"; It will be implemented on January 1); in the Twelfth Five-Year Development Plan of the pharmaceutical industry, it is pointed out that the pharmaceutical industry should develop greenly. Therefore, the proper disposal of the waste bacteria residue produced by antibiotics is ...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12P19/62C12R1/465
CPCC12N1/06C12P19/62
Inventor 刘俭国孟新梅侯虹任真邢继红陈金春王建国赵臻张琪孙玉杰鲍金庆李磊
Owner TOPFOND PHARMA CO LTD
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