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Fish meal proteolytic products, its preparation process and application

A protein hydrolyzate, fish meal technology, applied in the field of protein hydrolyzate, can solve problems such as less protein hydrolyzate

Inactive Publication Date: 2006-02-08
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing protein hydrolyzate with herbicidal activity is relatively small, to provide a kind of fishmeal protein hydrolyzate which can inhibit the germination of weed seeds and its preparation method. Herbicides that Inhibit Germination of Grass Seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] After adding deionized water to the fish meal, shake in a constant temperature oscillator at 60°C. After the temperature of the solution in the triangular flask is kept constant, adjust the pH of the solution to 8.0 with 20% ammonia water to become a substrate, and fish meal by mass ratio: deionized water=1 : 9; add alkaline protease reaction in the substrate, shake constantly in the reaction process, the temperature is kept on 60 ℃, and dropwise add 20% ammonia water to maintain the pH value of reaction system at 8.0, and the reaction time is 6h, according to mass ratio bottom Substance: alkaline protease = 1:2; the reaction solution was placed in a water bath at 100°C and heated for 15 minutes to inactivate the enzyme, then centrifuged at 4000r / min for 20 minutes, the supernatant was frozen and dried into a dry powder. Refrigerate and set aside.

[0017] The fishmeal used is FAQ fishmeal imported from Peru. The raw materials of the fishmeal are Anchovy (Engraulis Ring...

Embodiment 2

[0028] Similar to Example 1, the difference is that after adding deionized water to the fish meal, it is shaken in a constant temperature oscillator at 60° C. After the temperature of the solution in the conical flask is kept constant, adjust the pH of the solution to 7.8 with 20% ammonia water to become a substrate, press Mass ratio fish meal: deionized water=1:8; add alkaline protease reaction in the substrate, shake constantly during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 7.8, The reaction time is 5 hours, according to the mass ratio of substrate:alkaline protease=1:1; the reaction solution is placed in a water bath at 100°C and heated for 10 minutes to inactivate the enzyme, then centrifuged at 3800r / min for 15 minutes, and the supernatant is frozen and dried into Dry powder, keep refrigerated for later use.

Embodiment 3

[0030] Similar to Example 1, the difference is that after adding deionized water to the fish meal, it is shaken in a constant temperature oscillator at 60°C. After the temperature of the solution in the conical flask is kept constant, adjust the pH of the solution to 8.5 with 20% ammonia water to become a substrate, press Mass ratio fish meal: deionized water = 1: 10; add alkaline protease reaction in the substrate, shake constantly during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 8.5, The reaction time is 8 hours, according to the mass ratio of substrate:alkaline protease=1:3; the reaction solution is placed in a 100°C water bath and heated for 20 minutes to inactivate the enzyme, then centrifuged at 4200r / min for 25 minutes, and the supernatant is frozen and dried into Dry powder, keep refrigerated for later use.

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Abstract

Provided are fish meal proteolytic products, its preparation process and application, wherein the preparing process comprises, charging deionized water into fish meal, stirring in a container, charging ammonia to adjust the pH of the solution, charging basified protease for reaction, then charging ammonia to maintain the pH of the reaction system, water-bathing the reaction solution, removing enzyme, centrifuging, freeze drying the supernatant fluid into dried powder, finally freezing for later use.

Description

technical field [0001] The invention relates to a protein hydrolyzate, in particular to a hydrolyzate prepared from animal feed fishmeal protein, a preparation method and its application in preemergence control of weeds. Background technique [0002] Herbicides have been widely used to control weeds in farmland, orchards and urban lawns. There are many types of herbicides, but most of them are chemical herbicides produced by chemical synthesis. Although chemical herbicides can control the growth and spread of weeds to a limited extent, their pollution to soil and groundwater is becoming increasingly apparent. At the same time, repeated use of chemical herbicides can easily lead to drug resistance of weeds. Therefore, it is an inevitable trend in the development of herbicides to produce natural herbicides without toxic and side effects. In recent years, people have successively discovered that some natural active peptides and proteolytic polypeptides in some organisms have h...

Claims

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Application Information

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IPC IPC(8): A23J3/04A23J3/30A01N63/02
Inventor 杨剑卢昌义于兴娜叶勇
Owner XIAMEN UNIV
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