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Pantoea alhagi and application thereof

A technology of pantobacter and camel, which is applied to pantobacter camel and its application field, and can solve problems such as limiting the commercial development value of microbial fertilizers

Active Publication Date: 2020-10-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most plant growth promoters that secrete EPS are Gram-negative, such as Pseudomonas, Pantoea, and Klebsiella, etc., due to their inability to transform into dormant spores similar to Bacillus long-term storage in a state that often limits its commercial development value as a microbial fertilizer

Method used

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  • Pantoea alhagi and application thereof
  • Pantoea alhagi and application thereof
  • Pantoea alhagi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A preparation method of exopolysaccharide derived from Pantoea camelidum XK-11

[0036] Pick a single colony of P.alhagi XK-11 and place it in fresh LB liquid medium, culture it overnight at 37°C and 200rpm to obtain a seed solution. The seed liquid is transferred in the fermentation medium (40g / L sucrose; 5g / L peptone; 2g / L K 2 HPO 4; 2.33g / L n-hexane, 5.36g / L n-heptane, 9.06g / L n-hexadecane), then, the fermentation process temperature control is 30 ℃, pH is 7.0, ventilation is 0.8vvm, stirring speed is 800rpm , fermented for 24 hours to obtain the fermentation broth of Pantoea camelini XK-11 exopolysaccharide, and the exopolysaccharide yield of XK-11 in the fermentation broth was 21.8g / L.

[0037] The fermented P.alhagi XK-11 fermentation broth was subjected to in-situ high temperature (110° C.) oil bath viscosity reduction treatment for 15 minutes. Subsequently, it was subjected to wall breaking treatment by a high-pressure homogenizer to reduce viscosit...

Embodiment 2

[0038] The monosaccharide component determination of embodiment 2 exopolysaccharide

[0039] Weigh 10 mg of the dried exopolysaccharide obtained in Example 1, and after complete acid hydrolysis with trifluoroacetic acid, dry it with a nitrogen blower; then use methanol to redissolve and dry it again, repeat three times to obtain the monosaccharide component . The monosaccharide components were acetic acidified into sugar nitrile acetate derivatives, and the monosaccharide components were analyzed by gas chromatography-mass spectrometry (GC-MS).

[0040] The experimental results showed that the exopolysaccharide was composed of glucose, galactose and mannose. Among them, the molar ratio of glucose is the highest, which is 56.3%; the ratio of galactose is 29.6%; and the ratio of mannose is 14.1%.

Embodiment 3

[0041] Example 3 Exopolysaccharide Enhances Drought Resistance of Rice Seedlings

[0042] In the present invention, rice is taken as an example. The dried exopolysaccharide obtained in Example 1 is first configured into an aqueous solution for rice at the seedling stage, and then sprayed. The specific treatment is as follows:

[0043] The rice seeds were surface sterilized with 2.5% (g / mL) sodium hypochlorite aqueous solution for 20 minutes, and then washed 3 times with deionized water. The sterilized seeds were germinated at 22°C for 3 days in the dark. The seeds were randomly transferred to hydroponic boxes containing Hoagland's solution, 100 seedlings per box. Two weeks after hydroponics, the rice seedlings were divided into four groups, which were sprayed with 50 mL deionized water (control group), 20 ppm (EPS 20), 50 ppm (EPS 50) and 100 ppm (EPS 100) of exopolysaccharide. At the same time, 20% (g / mL hydroponic solution volume) PEG-6000 was added to the hydroponic solut...

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Abstract

The invention discloses pantoea alhagi and application thereof. The pantoea alhagi is classified and named as Pantoea alhagi, the strain name is XK-11, the pantoea alhagi is preserved in the China General Microbiological Culture Collection Center on March 29th, 2018, and the preservation number is CGMCC NO.15526. In the invention, the exopolysaccharide is derived from fermentation of the pantoea alhagi XK-11, and has the advantages of stable source, low cost, environmental friendliness and the like. According to the invention, the exopolysaccharide can enhance and promote the plant growth andenhance the abiotic stress resistance of crops by promoting the photosynthesis of plants, enhancing the proline content of the plants, reducing the MDA content of the plants and the like.

Description

technical field [0001] The invention belongs to the technical field of plant biological stimulants, and relates to Pantobacter camelis and its application. Background technique [0002] Drought is an extreme abiotic stress condition. Globally, the proportion of arable land affected by drought more than tripled between the 1970s and early 2000s, and it is projected that by 2050, the area of ​​arable land affected by drought will reach 50%, posing a huge threat to global crop yields. Similarly, sudden low temperature weather is a typical environmental factor affecting the growth of various crops. Many crops are affected by sudden low temperature weather, resulting in reduced crop quality, reduced yield, or even extinction. Therefore, improving the drought resistance and cold resistance of crops is of great significance for normal agricultural production. [0003] Exopolysaccharide (EPS) is a kind of carbohydrate polymer produced by microbial fermentation. Owing to their di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C08B37/00A01N43/16A01P21/00C12R1/01
CPCC12N1/20C12P19/04C08B37/0003A01N43/16C12R2001/01C12N1/205
Inventor 雷鹏孙良徐虹李莎杨燕波王瑞续晓琪
Owner NANJING UNIV OF TECH
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