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Biodegradation method for aflatoxin in gunited corn bran

A technology for spraying corn husks and aflatoxins, which is applied in animal feed, animal feed, food science, etc., can solve the problems of low enzyme yield and activity, and toxins not being completely degraded.

Pending Publication Date: 2020-10-27
SHANGHAI HONGYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are relatively few gene reports related to aflatoxin decomposing enzymes, and the yield and activity of the enzymes are not too high, and the toxins cannot be completely degraded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of biodegradation method to the aflatoxin in sprayed corn bran, its steps are as follows:

[0022] A. Soak corn husks

[0023] Immerse the corn husks that have not been mildewed through human observation into the corn soaking solution;

[0024] B. Biodegradation

[0025] The aflatoxin degradation treatment of corn bran was carried out by using the crude protein extract of Flavobacterium aurantiacus and aqueous solution, the crude protein extract of Flavobacterium aurantiacus in the aqueous solution accounted for 10%, and the crude extracellular extraction of Stenotrophomonas maltophilia medium The liquid was mixed with sprayed corn husks for 58 hours and heated to 27 degrees;

[0026] C. Ultraviolet detoxification

[0027] Use ultraviolet rays with a radiation intensity of 90W / m³ to irradiate and detoxify the corn husks flowing into the drying equipment;

[0028] D. Finished product

[0029] The existing drying equipment is used to dry the sprayed corn husks...

Embodiment 2

[0032] A kind of biodegradation method to the aflatoxin in sprayed corn bran, its steps are as follows:

[0033] A. Soak corn husks

[0034] Immerse the corn husks that have been judged to be partly mildewed by human eyes into the corn soaking liquid;

[0035] B. Biodegradation

[0036] The crude protein extract of Flavobacterium aurantiacus and aqueous solution were used to degrade corn husks for aflatoxin degradation. The crude protein extract of Flavobacterium aurantiaca in the aqueous solution accounted for 20%, and the extracellular crude extraction of Stenotrophomonas maltophilia medium The liquid was mixed with sprayed corn husks for 60 hours and heated to 30 degrees;

[0037] C. Ultraviolet detoxification

[0038] Use ultraviolet rays with a radiation intensity of 100W / m³ to irradiate and detoxify the corn husks flowing into the drying equipment;

[0039] D. Finished product

[0040] The existing drying equipment is used to dry the sprayed corn husks and form a so...

Embodiment 3

[0043] A kind of biodegradation method to the aflatoxin in sprayed corn bran, its steps are as follows:

[0044] A. Soak corn husks

[0045] The corn husks that have been judged to be half moldy by human eyes are immersed in the corn soaking solution after being irradiated with ultraviolet rays with a radiation intensity of 100W / m³;

[0046] B. Biodegradation

[0047] The crude protein extract of Flavobacterium aurantiacus and aqueous solution were used to degrade corn bran for aflatoxin degradation. The crude protein extract of Flavobacterium aurantiaca in the aqueous solution accounted for 25%, and the extracellular crude extraction of Stenotrophomonas maltophilia medium The solution was mixed with sprayed corn husks for 65 hours, and heated to 35 degrees for incubation;

[0048] C. Ultraviolet detoxification

[0049] Use ultraviolet rays with a radiation intensity of 120W / m³ to irradiate and detoxify the corn husks flowing into the drying equipment;

[0050] D. Finished...

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PUM

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Abstract

The invention discloses a biodegradation method for aflatoxin in gunited corn bran, and relates to the technical field of feed processing. The biodegradation method for the aflatoxin in the gunited corn bran is characterized in that the aflatoxin in the gunited corn bran is degraded by flavobacterium aurantiacum and culture medium extracellular crude extract liquid and is subjected to ultraviolettreatment, wherein the flavobacterium aurantiacum is lactoperoxidase, and the ratio of flavobacterium aurantiacum crude protein extract in an aqueous solution is 10-40%; and the culture medium extracellular crude extract liquid is stenotrophomonas maltophilia culture medium extracellular crude extract liquid. According to the biodegradation method for the aflatoxin in the gunited corn bran, afterthe flavobacterium citrinum protein extract is mixed with the aqueous solution, the aflatoxin B1 in the corn bran is degraded, and the ratio of the aflatoxin subjected to primary degradation in the corn bran is reduced to 76%; and biological enzyme degradation is performed on the aflatoxin by using the extracellular crude extract liquid.

Description

technical field [0001] The invention relates to the technical field of feed processing, in particular to a biodegradation method for aflatoxin in sprayed corn husks. Background technique [0002] Aflatoxins are mainly secondary metabolites biosynthesized by complex enzymatic reactions of gene homologous fungi such as Aspergillus flavus and Aspergillus parasiticus under certain environmental conditions. A total of 18 species have been identified so far, which are divided into two categories: B family and G family. Metabolism in animals can produce aflatoxin M1, M2 and other homologous derivatives. It causes serious pollution to food and feed, and aflatoxin B1 is the most toxic substance among aflatoxins. Aflatoxins mainly act on the liver and cause liver lesions. Eating food containing aflatoxin can cause acute poisoning of humans and animals and cause diseases, and aflatoxin is a strong carcinogen, causing gene mutation and deformity, which seriously threatens the health o...

Claims

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Application Information

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IPC IPC(8): A23K10/30A23L5/20A23L5/30
CPCA23K10/30A23L5/28A23L5/36A23L5/20Y02P60/87
Inventor 张玉国张楠楠
Owner SHANGHAI HONGYUAN BIOTECH
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