Functional amino acid enzyme and preparation method thereof

An amino acid and enzyme technology, applied in the field of bioengineering, can solve problems such as high operating costs and product risks

Inactive Publication Date: 2020-10-30
SHANDONG BAIDE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, domestic dead animals (pigs, chickens, cattle and sheep, etc.) have reached about 5 million tons per year according to statistics. These dead animals are mainly treated by high temperature or acid hydrolysis, but this treatment method has high operating costs. Bottleneck issues such as product risk
[0005] At present, chemical fertilizers such as nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer in traditional agricultural production can no longer meet the needs of modern agricultural development. Green, multi-effect, and pollution-free functional biological fertilizers have attracted more and more attention from agricultural authorities and practitioners, such as Microbial fertilizer, amino acid fert

Method used

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  • Functional amino acid enzyme and preparation method thereof
  • Functional amino acid enzyme and preparation method thereof
  • Functional amino acid enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Put 1000kg of slaughtered chicken intestines into the crushing device, carry out crushing treatment, and crush the material to below 20 orders. Then transport to the mincing device for mincing treatment, mincing to below 100 mesh. The crushed material is sterilized at 100°C for 1 hour. Stir continuously during the sterilization process, and the stirring frequency is 3r / min. Add 1% glucose by mass to the sterilized material, and cool down to 50°C. After cooling down, add 0.1% compound enzyme preparation (0.5 part of acid protease and 0.5 part of lipase), stir the enzymolysis for 3 hours, stirring frequency 5r / min, and adjust the pH value in the scope of 6.0~7.0. The prepared slurry is then transported to an aerobic fermentation container, inoculated with 0.1% special fermenter A agent (0.3 part of Bacillus subtilis, 0.7 part of Bacillus licheniformis and 1.0 part of Saccharomyces cerevisiae), the stirring frequency is 5r / min, and the Into the air 0.4VVm, fermentation ...

Embodiment 2

[0051] Put 1000kg of the slaughtered chicken skeleton into the crushing device, carry out crushing treatment, and crush the material to below 20 orders. Then transport to the mincing device for mincing treatment, mincing to below 100 mesh. The minced material is sterilized. The sterilization temperature is 90°C, and the sterilization time is 1 hour. Stir continuously during the sterilization process, and the stirring frequency is 5r / min. Add 2% mass of glucose to the sterilized material, and cool down to 45°C. After cooling down, add 0.2% compound enzyme preparation (0.7 part of acid protease and 0.3 part of lipase), stir the enzymolysis for 3 hours, stirring frequency 5r / min, and adjust the pH value in the scope of 6.0~7.0. The prepared slurry was transported to an aerobic fermentation container, inoculated with 0.1% special fermentation bacteria A agent (0.3 parts of Bacillus subtilis, 0.3 parts of Bacillus licheniformis and 1.5 parts of Saccharomyces cerevisiae), and the...

Embodiment 3

[0055] Put 1000kg of processed aquatic fish offal into the crushing device for crushing treatment, and crush the material to below 20 mesh. Then the material is sent to the crushing device for crushing treatment, and the crushing is below 100 mesh. The minced material is sterilized, the sterilization temperature is 80°C, and the sterilization time is 1 hour. Stir continuously during the sterilization process, and the stirring frequency is 5r / min. Add 2% glucose by mass to the sterilized material, and cool down to 55°C. After cooling down, add 0.3% compound enzyme preparation (0.4 part of acid protease and 0.6 part of lipase), stir and enzymolyze for 3 hours, stirring frequency 5r / min, and adjust the pH value in the scope of 6.0~7.0. Transfer the slurry prepared in the step to an aerobic fermentation container, inoculate with 0.1% special fermentation bacteria A agent (0.6 parts of Bacillus subtilis, 0.6 parts of Bacillus licheniformis and 0.8 parts of Saccharomyces cerevisia...

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Abstract

The invention discloses a preparation method of a functional amino acid enzyme. The method comprises the following steps: crushing an animal-derived protein raw material, and using acid protease and lipase for treatment; then carrying out aerobic fermentation by using bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae, carrying out anaerobic fermentation by using enterococcus faecalis, lactobacillus plantarum, lactobacillus acidophilus and streptococcus thermophilus after the aerobic fermentation is finished, and converting into a functional amino acid enzyme for feed or anagricultural functional amino acid enzyme fertilizer after microbial fermentation; the invention further discloses characteristics and application of the functional amino acid enzyme. According to the method, harmless treatment and resource utilization can be carried out on animals died of illness, the defects in the prior art are overcome, and the method has the advantages of being environmentally friendly, low in treatment cost, high in product additional value and wide in product application range and is a technology worthy of being popularized in the industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a functional amino acid enzyme and a preparation method thereof. Background technique [0002] my country is a country that lacks protein resources. China’s per capita share of land-based protein resources is less than one-third of the world’s average level. Because my country has a large population and little land, the arable farmland is mainly used for food production that guarantees energy quality. Soybean More than 85% of plant-based protein resources are imported, and raw materials to ensure animal protein production mainly rely on imported soybean meal. Therefore, the waste protein resources of agricultural and animal husbandry industries such as slaughter by-products / aquatic product processing by-products / disease dead animals on land need to be well utilized and are an important supplement to protein resources. [0003] While the domestic animal products and aquatic...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K20/189A23K20/147A23K10/26A23K10/30A23K20/163A23K10/33C05F17/20C05F17/40C05F17/50C05G3/00C05G3/60C05G5/20
CPCA23K10/12A23K20/189A23K20/147A23K10/26A23K10/30A23K20/163A23K10/33C05F1/005C05F17/20C05F17/40C05F17/50C05G3/00C05G3/60C05G5/20A23V2400/113A23V2400/225A23V2400/249A23V2400/169C05F11/00Y02W30/40Y02P60/87
Inventor 何永聚季维峰张希庆张洪钢董明刑天民张春平韩安阳韩文立王孟丽
Owner SHANDONG BAIDE BIOLOGICAL TECH
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