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High-purity enzyme modified stevioside and preparation method thereof

A stevioside, high-purity technology, applied in the field of stevioside, can solve the problems of uneven purity of stevioside, limitation of stevioside, complicated operation, etc., and achieve the effect of improving the taste, expanding the scope of application, and simple operation

Inactive Publication Date: 2020-10-30
JIANGSU STEVEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing enzyme modification technology is complicated to operate, and the purity of the finally obtained enzyme modification steviol glycosides is different, and the content is low, which limits the application of enzyme modification steviosides

Method used

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  • High-purity enzyme modified stevioside and preparation method thereof
  • High-purity enzyme modified stevioside and preparation method thereof
  • High-purity enzyme modified stevioside and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Step S1: Dissolve steviol glycosides and cyclodextrin in water at a mass ratio of 1:1.2, and the mass concentration of steviol glycosides is 10%;

[0039] Step S2: Add cyclodextrin glucosyl invertase to the solution of step S1, the addition amount is 2% of the addition amount of steviol glycosides, and stir evenly;

[0040] Step S3: React the solution of step S2 at 65°C for 28 hours;

[0041] Step S4: After the reaction of step S3 is completed, add cyclodextrin to continue the reaction for 5 hours, and the added amount is 12% of the amount of cyclodextrin in step S1;

[0042] Step S5: heating the reactant in step S4 to 95°C to inactivate the enzyme;

[0043] Step S6: After cooling, the reaction solution of step S5 is filtered with activated carbon to remove color and impurities;

[0044] Step S7: The solution obtained in step S6 is formulated into a solution with a mass concentration of 3%, and the adsorption resin is oversized until the solution is completely adsorbed at a flow r...

Embodiment 2

[0048] Step S1: Dissolve steviol glycosides and cyclodextrin in water at a mass ratio of 1:1, and the mass concentration of steviol glycosides is 12%;

[0049] Step S2: Add cyclodextrin glucosyl invertase to the solution of step S1, the addition amount is 2% of the addition amount of steviol glycosides, and stir evenly;

[0050] Step S3: React the solution of step S2 at 68°C for 30 hours;

[0051] Step S4: After the reaction of step S3 is completed, add cyclodextrin to continue the reaction for 5 hours, and the added amount is 12.5% ​​of the amount of cyclodextrin in step S1;

[0052] Step S5: heating the reactant in step S4 to 95°C to inactivate the enzyme;

[0053] Step S6: After cooling, the reaction solution of step S5 is filtered with activated carbon to remove color and impurities;

[0054] Step S7: The solution obtained in step S6 is prepared into a solution with a mass concentration of 4%, and the adsorption resin is oversized until the solution is completely adsorbed. The flow r...

Embodiment 3

[0058] Step S1: Dissolve steviol glycosides and maltodextrin in water at a mass ratio of 1:0.5, and the mass concentration of steviol glycosides is 1%;

[0059] Step S2: Add cyclodextrin glucosyltransferase and α-amylase to the solution of step S1 to form a combined enzyme at a mass ratio of 1:1, the addition amount is 1% of the addition amount of steviol glycosides, and the mixture is evenly stirred;

[0060] Step S3: React the solution of step S2 at 60°C for 48 hours;

[0061] Step S4: After the reaction of step S3 is completed, add maltodextrin to continue the reaction for 5 hours, and the added amount is 10% of the amount of maltodextrin in step S1;

[0062] Step S5: heating the reactant in step S4 to 90°C to inactivate the enzyme;

[0063] Step S6: After cooling, the reaction solution of step S5 is filtered with activated carbon to remove color and impurities;

[0064] Step S7: The solution obtained in step S6 is formulated into a solution with a mass concentration of 1%, and the ad...

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Abstract

The invention belongs to the technical field of stevioside, and provides a preparation method of high-purity enzyme modified stevioside, which comprises the following steps: dissolving stevioside anddextrin in water, adding a reaction enzyme, uniformly stirring, reacting for 24 to 48h under the condition of 60 to 70 DEG C, adding dextrin to continuously react for 5 hours, wherein the adding amount is 10%-20% of the amount of dextrin, raising the temperature to 90 to 105 DEG C, inactivating the enzyme, after cooling, filtering with activated carbon to remove color and impurities, preparing thesolution into macroporous adsorption resin with proper concentration, enabling the solution to pass through the macroporous adsorption resin until the solution is completely adsorbed, washing the adsorption column with water of which the volume is twice that of the adsorption column, eluting with 40-50% ethanol solution of which the volume is 4 times that of the adsorption column, and eluting anunreacted stevioside and enzyme modified stevioside mixture between the macroporous adsorption resin, when the ultraviolet absorbance of the effluent is greater than 0.1-0.3, collecting the effluent of 1-1.5 BV, concentrating the effluent, and spraying to dry to obtain the enzyme-modified stevioside.

Description

Technical field [0001] The invention relates to the technical field of stevioside, in particular to a high-purity enzyme modified steviol glycoside and a preparation method thereof. Background technique [0002] Steviol glycosides (also known as stevioside) is a mixture of multi-component sweet glycosides extracted from the leaves of the natural Asteraceae herb Stevia rebaudiana. It is the main flavoring substance in stevia. It is used for food sweetness in production. Flavoring agent. Steviol glycosides mainly include eight glycosides including stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, dukside and stevioside . Stevia is pure natural (from the pure natural plant stevia), high sweetness (250-450 times of sucrose), low calorie (only 1 / 300 of white sugar), economical use (cost is only 1 / 3 of sucrose) ), good stability (heat resistance, acid resistance, alkali resistance, not easy to decompose), high safety (no toxic side effects) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/56C07H15/256C07H1/00C07H1/06
CPCC07H1/00C07H1/06C07H15/256C12P19/56
Inventor 周爱琴周爱兵于茂兰张璐璐
Owner JIANGSU STEVEN BIOTECHNOLOGY CO LTD
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