Unlock instant, AI-driven research and patent intelligence for your innovation.

Hydrogel capsule

A gel and cell group technology, applied in the direction of metabolic diseases, prostheses, fusion cells, etc., can solve the problems of removing cells that have not been studied and unknown

Inactive Publication Date: 2020-10-30
KYOTO UNIV +1
View PDF19 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, until now, highly proliferative cells have been mixed in insulin-producing cell populations or pancreatic β-cell populations obtained by further inducing differentiation of endocrine progenitor cell populations derived from pluripotent stem cells or cell populations at subsequent differentiation stages. This fact is not known and how to remove this cell has not been studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hydrogel capsule
  • Hydrogel capsule
  • Hydrogel capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0181] Example I: Reduction / Proliferation Inhibition of Off-Target Cells (Ki67 Positive Cells) in Grafts by Transplantation of Endocrine Progenitor Cells Embedded in Alginic Acid-Containing Gels (I)

[0182] 1. Method

[0183] (1) Embedding endocrine progenitor cells in a gel containing alginic acid

[0184] Endocrine progenitor cells prepared by inducing differentiation of human iPS cells into endocrine progenitor cells were mixed with a 3% by weight alginic acid (PRONOVA SLG100) solution, and the cell dispersion was filled in 2 And in a circular mold with a thickness of about 500 μm, it was cross-linked with strontium to prepare a gel sheet containing alginic acid in which cells were dispersed and embedded.

[0185] (2) In vivo transplantation of endocrine progenitor cells

[0186] Immunodeficient NOD / SCID mice were treated with bFGF at the transplantation site by a known method to induce angiogenesis, and then the endocrine progenitor cell-embedded gel sheet obtained in (...

Embodiment II

[0193] Example II: After Insulin-Producing Cells Comprising Proliferative Cells Embedded in Alginic Acid-Containing Gel and Transplanted, Off-target Proliferative Cells Decrease

[0194] 1. Method

[0195] (1) Embedding insulin-producing cells in a gel containing alginic acid

[0196] Insulin-producing cells prepared by inducing differentiation of human iPS cells into insulin-producing cells were mixed with a 3% by weight alginic acid (PRONOVA SLG100) solution, and the cell dispersion was filled in 2 A circular mold with a thickness of about 500 μm was cross-linked with strontium to prepare a gel sheet containing alginic acid in which cells were dispersed and embedded.

[0197] (2) In vivo transplantation of insulin-producing cells

[0198] Using immunodeficient NOD / SCID mice, the insulin-producing cell-embedded gel sheets obtained in (1) above were transplanted subcutaneously on their backs. After 6 months of transplantation, the grafts were removed.

[0199] (3) Evaluati...

Embodiment III

[0205]Example III: Reduction of off-target cells (Ki67-positive cells) in in vitro culture by embedding insulin-producing cells in alginic acid-containing gels (I)

[0206] 1. Method

[0207] (1) Embedding insulin-producing cells in a gel containing alginic acid

[0208] Insulin-producing cells prepared by inducing human iPS cells to differentiate into insulin-producing cells were mixed with different concentrations (0.5%-3% by weight) and grades (PRONOVA SLG100 (G / M ratio: ≥ 1.5), NOVATACH MVG GRGDSP (G / M ratio: ≥1.5), PRONOVA UP VLVM (G / M ratio: ≤1)) were mixed with alginic acid solution, and the cell dispersion was filled to an inner diameter of 1cm 2 , in a circular mold with a thickness of about 500 μm, and cross-linked with strontium aqueous solution or calcium aqueous solution to prepare a gel sheet containing alginic acid in which cells are dispersed and embedded. Elasticity (Pa) was measured with a rheometer (viscoelasticity measuring device).

[0209] [Table 2]

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The purpose of the present invention is to provide means for removing highly proliferative Ki67-positive cells that co-exist with differentiation-induced insulin-secreting cells. This method is for producing an insulin-producing cell population or a pancreatic [beta] cell population containing less than 3% of Ki67-positive cells, comprising embedding of an endocrine progenitor cell population or acell population at a later differentiation stage in a gel containing alginic acid for further differentiation.

Description

technical field [0001] The present invention relates to a method for removing highly proliferative Ki67-positive cells present in insulin-producing cell populations or pancreatic beta cell populations obtained by inducing differentiation of pluripotent stem cells. Background technique [0002] Research on the differentiation of induced pluripotent stem cells (such as iPS cells and ES cells) into insulin-secreting cells (such as insulin-producing cells and pancreatic β cells) for the treatment of diabetes is ongoing. [0003] So far, various techniques have been developed / reported to induce the differentiation of pluripotent stem cells into insulin-secreting cells. Non-Patent Document 1 shows that pancreatic progenitor cells derived from human ES cells can be cultured using an alginate-based scaffold, and it has been confirmed that the expression of insulin / PDX1, glucagon / The expression of somatostatin increased and the expression of β-cell-associated genes decreased. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/16A61K35/39C12N11/10
CPCC12N11/04A61K35/39C12N5/0678C12N2506/45C12N2533/74C12N2533/90C12N2533/72C12N2533/78C12N5/0677A61P3/10A61K9/06A61K47/36A61L27/20A61L27/3687A61L27/3804A61L27/54C12N5/0676C12N2501/90
Inventor 豊田太郎持田泰佑田上宽伊藤亮木本香哉小池悠介上野光
Owner KYOTO UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com