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Method for extracting bromelain from pineapple stems and leaves

A technology of bromelain, stems and leaves, applied in the field of enzyme extraction, can solve the problem of low quality of bromelain, achieve the effect of improving adsorption effect, quality and extraction efficiency

Active Publication Date: 2020-11-03
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the existing method of extracting bromelain from pineapple stems and leaves, in the process of extracting bromelain, there are still a large amount of bromelain remaining in pineapple stems and leaves, and the extracted bromelain contains some impurities, so that the extracted bromelain Low quality bromelain

Method used

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  • Method for extracting bromelain from pineapple stems and leaves

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Effect test

Embodiment 1

[0026] A method for extracting bromelain from pineapple stems and leaves, comprising the steps of:

[0027] Step S1: Rinse the pineapple stems and leaves twice, each time for 3 minutes, and then dry them. At a temperature of 3°C, keep warm for 10 minutes, then pulverize and add phosphate buffer solution. Under the condition of pH 5, After standing still for 10 minutes, filter and centrifuge the filtrate at a speed of 4000r / min for 15 minutes, take the supernatant to obtain a base solution, and keep the base solution at a temperature of 3°C for standby;

[0028] Step S2: Add the base liquid and extraction microspheres prepared in step S1 into a stirred tank, stir for 20 minutes at a rotation speed of 150r / min and a temperature of 3°C, and then raise the temperature to 8°C. After continuing to stir for 30 minutes, remove the filtrate by filtration, add the filter cake and sodium hydroxide solution to the reaction kettle until the pH value is 7, then add sodium chloride, and stir...

Embodiment 2

[0037] A method for extracting bromelain from pineapple stems and leaves, comprising the steps of:

[0038] Step S1: Rinse the pineapple stems and leaves for 3 times, each time for 4 minutes, and then dry them. After keeping the temperature at 4°C for 12 minutes, crush them and add phosphate buffer solution. Under the condition of pH 5, After standing still for 12 minutes, filter and centrifuge the filtrate at a speed of 4000r / min for 17 minutes, take the supernatant to obtain a base solution, and keep the base solution at a temperature of 4°C for standby;

[0039] Step S2: Add the base liquid and extraction microspheres prepared in step S1 into a stirred tank, and stir for 22 minutes at a rotation speed of 150 r / min and a temperature of 4°C, then raise the temperature to 9°C, After continuing to stir for 40 minutes, remove the filtrate by filtration, add the filter cake and sodium hydroxide solution to the reaction kettle until the pH value is 7, then add sodium chloride, and...

Embodiment 3

[0048] A method for extracting bromelain from pineapple stems and leaves, comprising the steps of:

[0049] Step S1: Rinse the pineapple stems and leaves for 3 times, each time for 4 minutes, and then dry them. After keeping the temperature at 4°C for 14 minutes, crush them and add phosphate buffer solution. Under the condition of pH 6, After standing still for 14 minutes, filter and centrifuge the filtrate at a speed of 5000r / min for 15-20 minutes, take the supernatant to obtain the base solution, and keep the base solution at a temperature of 4°C for standby;

[0050] Step S2: Add the base liquid and extraction microspheres prepared in step S1 into a stirred tank, stir for 25 minutes at a rotation speed of 200r / min, and a temperature of 4°C, then raise the temperature to 9°C, After continuing to stir for 50 minutes, remove the filtrate by filtration, add the filter cake and sodium hydroxide solution to the reaction kettle until the pH value is 7, then add sodium chloride, an...

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Abstract

The invention discloses a method for extracting bromelain from pineapple stems and leaves. According to the method, impurities in the extracted bromelain are completely removed through extraction twice, so that the quality of the bromelain is greatly improved, and an extraction microsphere is prepared in the extraction process. According to the extraction microsphere, carboxyl cellulose and amino-terminated silicone oil are under the action of 4-dimethylaminopyridine, so that carboxyl on the carboxyl cellulose and amino on the amino-terminated silicone oil react to form amide, further the amino-terminated silicone oil is fixed on the surface of cellulose, the cellulose has good adsorption capacity. The bromelain can be adsorbed, the amino-terminal silicone oil is further fixed on the surface of the cellulose, the amino-terminal silicone oil is a surfactant, the adsorption effect on the bromelain is further improved, and the cellulose is made into microspheres, so that the extracted microsphere can be repeatedly used, and the extraction efficiency of the bromelain is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme extraction, and relates to a method for extracting bromelain from pineapple stems and leaves. Background technique [0002] Pineapple has high nutritional value, rich in nutrients and functional substances, and there are many processed products. It is very popular in many fields such as food, medicine, health care products and daily necessities. Bromelain is the most valuable efficacy in pineapple. One of the ingredients can quickly decompose protein, tenderize meat, dissolve fibrin and blood clots blocked in tissues, improve local blood circulation, eliminate inflammation and edema, and is one of the main protease sources on the market. [0003] In the existing method of extracting bromelain from pineapple stems and leaves, in the process of extracting bromelain, there are still a large amount of bromelain remaining in pineapple stems and leaves, and the extracted bromelain contains some impuritie...

Claims

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Application Information

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IPC IPC(8): C12N9/50B01J20/24B01J20/28B01J20/30
CPCC12N9/63C12Y304/22032B01J20/24B01J20/22B01J20/28021
Inventor 周伟李积华曹玉坡刘义军黄晓兵廖良坤彭芍丹张利
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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