Tyrosinase inhibitor composition, skin brightening essence and preparation method of skin brightening essence
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A tyrosinase and inhibitor technology, applied in the field of daily chemicals, can solve the problems of easy discoloration of kojic acid, skin irritation, skin irritation, etc. Effect
Active Publication Date: 2020-11-10
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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[0004] In view of the prior art, hydroquinone, kojic acid and arbutin are often used as inhibitors to reduce the production of melanin, but hydroquinone is very irritating to the skin; kojic acid is easy to change color and also has certain irritation to the skin; The effect of arbutin is single and there are problems such as poor effect
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Embodiment 2-6
[0053] Dilute the extract of Phyllostachys antarctica with ultrapure water to obtain 5 groups of test solutions with different concentrations (final concentrations). When performing the test for inhibiting tyrosinase activity, the concentrations of the extracts of Phyllostachys antarctica are shown in Table 1 below.
[0054] Inhibition of tyrosinase activity by extracts from Phyllostachys antarctica. The tyrosinase activity is measured by the tyrosinase activity that catalyzes the production of dopaquinone from L-dopa. Specifically: Take 6 test tubes, add 0 μl, 50 μl, 100 μl, 200 μl, 300 μl, 400 μl of Plexiglas antarctica extract respectively, fill up to 1ml with phosphate buffer (0.5mM, PH=6.8), and then add 500μl tyrosine Acidase (50U / ml), water bath at 23°C for 10min, add 500μl L-DOPA (2.5mM) after the reaction, and measure the absorbance at a wavelength of 450nm with a UV spectrophotometer, denoted as A.
[0055] Take 6 test tubes, add 0 μl, 50 μl, 100 μl, 200 μl, 300 μl...
Embodiment 7-13
[0066] Take Plexiglas antarctica extract and Resurrection Grass extract as tyrosinase inhibitors. The Antarctica Antarctica extract and the resurrection grass extract are in a mass ratio of 1:9 (Example 7), 2:8 (Example 8), 2:3 (Example 9), 4:1 (Example 10) , 3:2 (Example 11), 8:2 (Example 12), and 9:1 (Example 13) were mixed to obtain a tyrosinase inhibitory composition with a total concentration of 10%. Determination of the inhibition of tyrosinase by the combination of the extracts of Phyllostachys antarctica and the extracts of P.
[0067] Take 10 test tubes, add 0μl, 0μl, 20μl, 40μl, 80μl, 100μl, 120μl, 160μl, 180μl, 200μl of P. , 20μl, 0μl Resurrection Grass extract, and make up to 1ml with phosphate buffer (0.5mM PH=6.8), then add 500μl tyrosinase (50U / ml), water bath at 23°C for 10min, add 500μl L- For DOPA (2.5mM), the absorbance was detected at a wavelength of 450nm by an ultraviolet spectrophotometer, and it was recorded as X.
[0068] Take 6 test tubes, add 0 μl...
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Abstract
The invention discloses a tyrosinase inhibitor composition, skin brightening essence and a preparation method thereof. The tyrosinase inhibitor composition is prepared from, by mass ratio, durvillea antartica extract and resurrection callus culture extract with the ratio being 1:4-10, and preferably the ratio of the durvillea antartica extract to the resurrection callus culture extract is 1:9. Theskin brightening essence provided by the invention comprises 0.5%-20% of the tyrosinase inhibitor composition, 5-20% of a humectant, 0.2-1% of a rheological regulator, 1%-5% of water-soluble grease,0.2-1% of a skin conditioner, 0.01-1% of a chelating agent, 0.5-1% of a preservative, 0.01-2% of a pH regulator, 0.05-0.5% of an aromatic and the balance of water, totaling 100%, The tyrosinase inhibitor composition is composed of the durvillea antartica extract and the resurrection callus culture extract, the mass ratio of the durvillea antartica extract to the resurrection callus culture extractis 1: (4-10) according to the mass ratio calculation, and the skin brightening essence comprises the tyrosinase inhibitor composition obtained through matching of the durvillea antartica extract andthe resurrection callus culture extract and is capable of inhibiting tyrosinase activity, achieving the whitening function and being free of a side effect on the human body.
Description
technical field [0001] The invention relates to the field of daily chemicals, in particular to a tyrosinase inhibitor composition, skin brightening essence and a preparation method thereof. Background technique [0002] Melanin is mainly produced by melanocytes in the basal layer of human skin epidermis, which can reduce the damage of ultraviolet rays to the skin. The content and distribution of melanin determine the color of the skin. However, the abnormal accumulation of melanin in the basal layer will cause hyperpigmentation and cause melasma, freckles, age spots, etc., affecting people's quality of life. With the deepening of research on melanin biosynthesis, researchers found that tyrosinase plays an important role in melanin biosynthesis. It is the main rate-limiting enzyme in the process of converting tyrosine and dopa to melanin. Its overexpression is the main cause of hyperpigmentation. Therefore, inhibiting the activity of tyrosinase can block the biosynthesis r...
Claims
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