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A nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application

A biomimetic nano, rabies virus technology, applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc.

Active Publication Date: 2021-09-17
吉林省金泰美迪生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Rabies virus can efficiently invade the central nervous system, and studies have found that this is mainly due to its unique elastic shape and the tightly wrapped layer of glycoproteins on its surface that can specifically target the acetylcholine receptors in the central nervous system, but It has not been reported that the unique morphology and high-efficiency invasion characteristics of rabies virus are applied in the field of medicine

Method used

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  • A nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application
  • A nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application
  • A nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0078] Nano-diagnosis and treatment preparations for central nervous system diseases based on rabies virus bionics, in which the matrix material metal-organic framework is MIL-101-NH 2 As an example, the targeting polypeptide takes RVG29 as an example, and its preparation method includes the following steps:

[0079] Weigh 15mg FeCl 3 ·6H 2 O was dissolved in 1 mL of absolute ethanol, and 25 mg of H 2 Dissolve BDC in 9mL ethanol, add 0.05% volume regulator water to the reaction system, react at 35°C for 1h, 12000rpm, 10min, centrifuge and ultrasonically redisperse, wash with ethanol several times to obtain MIL-101-NH 2 , abbreviated as MIL.

[0080] The obtained MIL (2 mg) was added to 2 mL of DOPA in chloroform (10 mg / mL), ultrasonically dispersed the MIL in chloroform, and the product was dispersed in 10 mL of chloroform after centrifugation and redispersion to wash away excess DOPA with chloroform. Add 20mg of DOPC, 2mg of DOPE, 10mg of chol, and 2mg of PEG-2000PE in pr...

Embodiment 2

[0083] Preparation of nano-therapeutic agents MIL-DOX@Lip-RVG29 and MIL-OXA@Lip-RVG29 for the diagnosis and treatment of glioma. When delivering doxorubicin DOX, just add 5 mg of DOX during the reaction process of preparing MIL, and other steps are the same as in Example 1; when delivering oxaliplatin OXA, mix the synthesized MIL, OXA and water in proportion and incubate for 24 hours. , add OXA, MIL-101-NH during incubation 2 , The mass ratio of water is 1:1:2, and other steps are the same as in Example 1. Finally, the nano-therapeutic agents MIL-DOX@Lip-RVG29 and MIL-OXA@Lip-RVG29 that can be used in the diagnosis and treatment of glioma were obtained. This method can be used to prepare and deliver other glioma drugs.

Embodiment 3

[0085] Preparation of nano-therapeutic agent MIL-MB@Lip-RVG29 for the diagnosis and treatment of Alzheimer's disease. When delivering the ROS scavenger methylene blue MB, mix the synthesized MIL, MB and water in proportion and incubate for 24 hours, add MB, MIL-101-NH during the incubation 2 , The mass ratio of water is 1:1:2, and other steps are the same as in Example 1. Finally, the nano-therapeutic agent MIL-MB@Lip-RVG29 that can be used in the diagnosis and treatment of Alzheimer's disease was obtained. This method can be used to prepare and deliver other Alzheimer's disease drugs.

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Abstract

The invention discloses a nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application. The polypeptide or nucleic acid aptamer targeting the central nervous system on the surface of the membrane. The preparation method is as follows: using MOFs as the delivery carrier, adjusting the synthesis conditions to control its size and shape to imitate the shape of rabies virus, and coating the surface of MOFs with a lipid bilayer membrane after loading the diagnostic agent and modifying it with the target central nervous system. The peptide or nucleic acid aptamer forms an infective surface that mimics the glycoprotein composition of rabies virus, thereby improving the ability of nano-diagnosis and treatment preparations to cross the blood-brain barrier and target the central nervous system. The present invention endows the diagnosis and treatment function of the nano-preparation by delivering the diagnosis and treatment agent in the channel of the matrix material MOFs, imitating the rabies virus to construct the corresponding nano-treatment preparation, which can effectively improve the problem that the existing nano-treatment preparation cannot efficiently cross the blood-brain barrier to reach the lesion. Difficulties, improve the efficiency of diagnosis and treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a nano-diagnosis and treatment preparation based on bionic rabies virus and its preparation method and application. Background technique [0002] According to statistics, the number of deaths due to diseases of the central nervous system accounts for about 12% of the total number of deaths in the world, which is mainly attributed to the existence of the blood-brain barrier (BBB), which leads to a very low concentration of corresponding diagnostic and therapeutic drugs in the brain, which is only 1-4% of the injection dose is difficult to reach the lesion site to play a role in diagnosis and treatment, so the mortality rate of central system diseases remains high. Using nanocarriers to deliver diagnostic and therapeutic drugs and modifying targeting elements that can efficiently target the blood-brain barrier can effectively achieve targeted enrichment of diagnostic and therape...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/06A61K47/26A61K31/555A61K31/5415A61P25/00A61P35/00A61P25/28
CPCA61K9/5123A61K9/5169A61K31/5415A61K31/555A61P25/00A61P25/28A61P35/00
Inventor 王忠良乔晁强张瑞丽王永东贾茜常湾湾薛腾飞纪仁川崔秀芳田捷
Owner 吉林省金泰美迪生物技术有限公司