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Earthworm polypeptide as well as preparation method and application thereof

A technology for earthworms and preparation steps, which is applied in the field of biotechnology or cosmetics, and can solve problems such as affecting effect and single product.

Pending Publication Date: 2020-11-10
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the product obtained by this method is pure, the product is relatively single, which affects its effect

Method used

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  • Earthworm polypeptide as well as preparation method and application thereof
  • Earthworm polypeptide as well as preparation method and application thereof
  • Earthworm polypeptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Clean up 500g of fresh earthworms to remove surface dirt and floating foam; soak the cleaned earthworms in deionized water for 24 hours, and change the water every 2 hours; Put into a freezer for freeze-drying, and crush after freezing to obtain earthworm particles with an average particle size of 60 meshes. The above pulverized earthworm particles were added to the deionized solution at a concentration of 3 g / mL, and after returning to room temperature, a slurry containing earthworm particles was obtained.

[0094] Lactobacillus, Bifidobacterium, and Saccharomyces cerevisiae were mixed and inoculated into the activation medium at an inoculation ratio of 1:2:1, and were cultured statically at 33°C for 24 hours for activation. Based on the total mass of the activation medium, the specific composition of the activation medium is 0.8% of beef extract, 1.0% of peptone, 0.5% of sodium chloride, 1.2% of glucose, 2.0% of agar, and the rest is water.

[0095] Inoculate the act...

Embodiment 2

[0104] Clean up 500g of fresh earthworms to remove surface dirt and floating foam; soak the cleaned earthworms in deionized water for 24 hours, and change the water every 2 hours; Put into the freezer for freeze-drying, and crush after freezing to obtain earthworm particles with an average particle size of 80 meshes. The above pulverized earthworm particles were added into deionized water at a concentration of 2 g / mL, and after returning to room temperature, a slurry containing earthworm particles was obtained.

[0105] Lactobacillus, Bifidobacterium, and Saccharomyces cerevisiae were mixed and inoculated into the activation medium at an inoculation ratio of 2:2:1, and were cultured statically at 34°C for 28 hours for activation. Based on the total mass of the activation medium, the specific composition of the activation medium is 0.8% of beef extract, 1.0% of peptone, 0.5% of sodium chloride, 1.2% of glucose, 2.0% of agar, and the rest is water.

[0106] Inoculate the activa...

Embodiment 3

[0112] Clean up 500g of fresh earthworms to remove surface dirt and floating foam; soak the cleaned earthworms in deionized water for 24 hours, and change the water every 2 hours; Put into the freezer for freeze-drying, and crush after freezing to obtain earthworm particles with an average mesh number of 70 meshes. The above pulverized earthworm particles were added into deionized water at a concentration of 4 g / mL, and after returning to room temperature, a slurry containing earthworm particles was obtained.

[0113] Lactobacillus, Bifidobacterium, and Saccharomyces cerevisiae were mixed and inoculated into the activation medium at an inoculation ratio of 2:1:1, and were cultured statically at 32°C for 32 hours for activation. Based on the total mass of the activation medium, the specific composition of the activation medium is 0.8% of beef extract, 1.0% of peptone, 0.5% of sodium chloride, 1.2% of glucose, 2.0% of agar, and the rest is water.

[0114] Inoculate the activate...

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Abstract

The invention provides earthworm polypeptide as well as a preparation method and application thereof. The preparation method of the earthworm polypeptide comprises the following steps: a fermentationstep: inoculating a mixed strain into slurry containing earthworm particles and performing fermentation culture to obtain fermentation liquid; And a separation step: performing separation treatment onthe fermentation liquid to obtain a separation product containing the earthworm polypeptide. The mixed strain comprises two or a combination of more than two of lactobacillus, bulgarian bacillus, bifidobacteria, pseudoalteromonas, candida utilis, saccharomyces cerevisiae, bacillus natto and bacillus subtilis. The earthworm polypeptide disclosed by the invention is more appropriate in molecular weight, high in safety, excellent in stability and high in transdermal absorption rate. Further, the earthworm polypeptide disclosed by the invention has a good promoting effect on growth or proliferation of cells in the skin, can improve the regeneration capacity of the cells in the skin and can improve the elasticity of the skin.

Description

technical field [0001] The invention belongs to the field of biotechnology or cosmetics, and specifically relates to a method for preparing earthworm polypeptides, earthworm polypeptides prepared by the preparation method and applications thereof. Background technique [0002] Covering the body surface, the skin acts as the first line of defense for the human body against external damage. While protecting various organs and tissues in the body from external harmful factors, it is also susceptible to various external environmental influences or the joint action of its own endogenous factors. And produce signs of aging such as sagging skin, dullness, deepened wrinkles, and pigmentation. [0003] Collagen is the main component of the dermis and the most abundant protein in the human body. The proportion of various types of collagen in human skin also changes with age. In young skin, type I collagen and type III collagen account for 80% of the total skin collagen respectively. ...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P39/06A61K8/64A61Q19/08C12P39/00C12P21/00C07K1/34C12R1/225C12R1/01C12R1/72C12R1/865C12R1/125
CPCA61K38/1767A61P39/06A61K8/64A61Q19/08C12P39/00C12P21/00C07K1/34A61K2800/85
Inventor 陈剑晖张慧崔英云黄建珍李传茂张楚标曾伟丹张伟杰
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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