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Method for preparing partial glyceride through enzymatic glycerine hydrolysis of triglyceride

A technology for partial glycerides and triglycerides, which is applied in the field of enzymatic glycerolysis of triglycerides to prepare partial glycerides, can solve the problems of increasing production steps and costs, and achieves simplified separation and purification procedures, low cost consumption and high glycerol effect of tolerance

Inactive Publication Date: 2020-11-10
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In a solvent-free system, the viscosity of glycerin and its immiscibility with oils will affect the progress of the reaction. In an organic system, although the reaction system is relatively uniform, the production steps and costs are virtually increased.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 3g of weakly polar macroporous adsorption resin, 6g of Tris-HCL buffer, and 0.3g of free lipase MAS1-IM, mix them at 25°C for 3 hours, then filter and dry to obtain immobilized enzyme MAS1-IM, put them in 4 ℃ refrigerator for later use.

[0032] Take 8.28g of soybean oil, 1.72g of glycerol, and 2g of the above-mentioned fixed lipase MAS1-IM in a flask, place them in an air-bath shaker, and at a temperature of 40°C, the speed of the air-bath shaker is 150rpm / min After 4.5 hours of reaction, the reaction was stopped. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 17.36%, the diglyceride content was 48.92%, and the monoglyceride content was 32.06%. Under the condition that the temperature is 140°C and the pressure is 10Pa, molecular distillation is carried out on t...

Embodiment 2

[0034] Take 5g of non-polar macroporous adsorption resin, 10g of Tris-HCL buffer, and 1g of free lipase MAS1-IM, mix and stir at 30°C for 3 hours, then filter and dry to obtain immobilized enzyme MAS1-IM, put it in 4°C Refrigerator for spare.

[0035] Take 7.62g soybean oil, 2.38g glycerol, and 2.5g of the above-mentioned fixed lipase MAS1-IM in a flask, place them in an air-bath shaker, and at a temperature of 50°C, the speed of the air-bath shaker is 150rpm / min The reaction was carried out for 4 hours, and the reaction was stopped. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 13.9%, the diglyceride content was 58.2%, and the monoglyceride content was 27.9%. Under the condition that the temperature is 150°C and the pressure is 13Pa, molecular distillation is carried out ...

Embodiment 3

[0037] Take 10g of ion exchange resin, 20g of Tris-HCL buffer, and 1.5g of free lipase MAS1-IM, mix and stir at 35°C for 4 hours, then filter and dry to obtain immobilized enzyme MAS1-IM, and put it in a 4°C refrigerator for later use.

[0038] Take 6.58g of soybean oil, 3.42g of glycerol, and 1g of the above-mentioned fixed lipase MAS1-IM in a flask, place them in an air bath shaker, and set the temperature at 60°C with the speed of the air bath shaker at 150rpm / min After reacting for 6 hours, stop the reaction. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 18.4%, the diglyceride content was 48.8%, and the monoglyceride content was 32.8%. Under the condition that the temperature is 150°C and the pressure is 13Pa, molecular distillation is carried out on the upper layer of ...

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Abstract

The invention discloses a method for preparing partial glyceride through enzymatic glycerine hydrolysis of triglyceride. The method comprises the following steps of (1) preparing immobilized lipase MAS1-IM; (2) performing enzyme catalysis glycerine hydrolysis to prepare partial glyceride, under the condition of being free from a solvent system, mixing components in parts by mass of 1-10 parts of vegetable oil, 1-4 parts of glycerine and 0.2-2.5 parts of the immobilized lipase MAS1-IM, and performing reaction at the temperature of 40-70 DEG C for 4-8 hours to obtain a product mixture; or underthe condition of the solvent system, mixing 1-10 parts of the vegetable oil with 1-4 parts of the glycerol, 13-39 parts of an organic phase reagent and 0.2-2.5 parts of the immobilized lipase MAS1-IM,and performing reacting at the temperature of 40-70 DEG C for 2-6 hours to obtain a product mixture; and (3) separating the product mixture to obtain the partial glyceride. The method has the advantages of being simple in process, high in reaction rate, high in product content and free from side reaction.

Description

technical field [0001] The invention relates to a method for enzymatic glycerolysis of triglycerides to prepare partial glycerides. Background technique [0002] Partial glyceride is a general term for diglyceride and monoglyceride, and both diglyceride and monoglyceride can be used as food additives. Monoglycerides can be used as emulsifiers in the fields of food, medicine and cosmetics, while diglycerides have been certified as a new type of healthy oil that is safe, non-toxic and side effects, and can effectively prevent fat accumulation and regulate body fat. Generally speaking, the production of partial glycerides can be divided into chemical method and biological enzymatic method. Although the chemical method is low in cost and easy to carry out large-scale production, it has the disadvantages of generating many by-products, large pollution, and high energy consumption for the reaction. The emergence of the biological enzyme method has solved these problems very well....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/08C12N9/20
CPCC12P7/6445C12N11/08C12N9/20C12Y301/01003
Inventor 王永华岳海鹏杨博王卫飞蓝东明罗日明
Owner SOUTH CHINA UNIV OF TECH
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