Method for preparing partial glyceride through glycerolysis reaction

Abstract

The invention discloses a method for preparing partial glyceride through a glycerolysis reaction. The method comprises the following steps: (1) preparing immobilized lipase of a Lipase MAS1 mutant, wherein the Lipase MAS1 mutant is MAS1-H108A, and the amino acid sequence of the Lipase MAS1 mutant is as shown in SEQ NO. 2; (2) preparing partial glyceride by enzyme catalysis glycerolysis reaction: mixing 1-10 parts by mass of vegetable oil, 1-4 parts by mass of glycerol, 0-40 parts by mass of organic phase reagent and 0.2-1.5 parts by mass of immobilized lipase, and reacting for 1-6 hours at the temperature of 40-55 DEG C to obtain a product mixture; and (3) separating the product mixture to obtain the partial glyceride. The method has the advantages of simple process, high reaction rate, high product content and no side reaction.

Description

technical field [0001] The invention relates to a method for preparing partial glycerides by glycerolysis reaction. Background technique [0002] Partial glyceride is a general term for diglyceride and monoglyceride, and both diglyceride and monoglyceride can be used as food additives. Monoglycerides can be used as emulsifiers in the fields of food, medicine and cosmetics, while diglycerides have been certified as a new type of healthy oil that is safe, non-toxic and side effects, and can effectively prevent fat accumulation and regulate body fat. Generally speaking, the production of partial glycerides can be divided into chemical method and biological enzymatic method. Although the chemical method is low in cost and easy to carry out large-scale production, it has the disadvantages of generating many by-products, large pollution, and high energy consumption for the reaction. The emergence of the biological enzyme method has solved these problems very well. The production ...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods of oil production are mainly divided into chemical method and biological enzymatic method. Due to the high energy consumption of chemical method, some pollution to the environment, and potential safety hazards, the preparation of oil by biological enzymatic method has become a research hotspot in recent years. Biological enzyme The preparation of fats and oils generally includes esterification, hydrolysis, and glycerol hydrolysis. Although esterification and hydrolysis can produce a mixture of diglycerides and monoglycerides, the hydrolysis method will produce a large amount of fatty acids and glycerol by-products, and the system The content of diglyceride and monoglyceride in the medium is low, but the production cost of the esterification method is high and the production rate of the product is low, which does not meet the production needs of the factory. High-efficiency glycerolysis method for production

[0004] The system of bio-enzymatic preparation of oil generally includes solvent-free system, organic solvent system, supercritical CO 2 system, etc., but because of the use of supercritical CO 2 The system is expensive, so this method is generally not used for production