Method for preparing partial glyceride through glycerolysis reaction

A technology of partial glyceride and glycerolysis, which is applied in the field of preparation of partial glyceride by glycerolysis reaction, can solve the problems of not meeting the production requirements, high energy consumption of chemical methods, low product generation rate, etc., and achieve good industrial prospects and high degree of reaction , the effect of high content

Active Publication Date: 2021-05-28
YHSN TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods of oil production are mainly divided into chemical method and biological enzymatic method. Due to the high energy consumption of chemical method, some pollution to the environment, and potential safety hazards, the preparation of oil by biological enzymatic method has become a research hotspot in recent years. Biological enzyme The preparation of fats and oils generally includes esterification, hydrolysis, and glycerol hydrolysis. Although esterification and hydrolysis can produce a mixture of diglycerides and monoglycerides, the hydrolysis method will produce a large amount of fatty acids and glycerol by-products, and the system The content of diglyceride and monoglyceride in the medium is low, but the production cost of the esterification method is high and the production rate of the product is low, which does not meet the production needs of the factory. High-efficiency glycerolysis method for production
[0004] The system of bio-enzymatic preparation of oil generally includes solvent-free system, organic solvent system, supercritical CO 2 system, etc., but because of the use of supercritical CO 2 The system is expensive, so this method is generally not used for production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 (solvent-free system)

[0032] Take 3g of ECR1030 resin, 6g of Tris-HCL buffer, and 0.3g of free lipase MAS1-V202F, mix them at 25°C for 3 hours, then filter and dry to obtain immobilized lipase MAS1-V202F, put them in a 4°C refrigerator for later use.

[0033] Take 8.28g of soybean oil, 1.72g of glycerol, and 1g of the above-mentioned fixed lipase MAS1-V202F in a flask, place them in an air-bath shaker, at a temperature of 40°C, and the speed of the air-bath shaker at 150rpm / min After reacting for 5 hours, stop the reaction. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 27.36%, the diglyceride content was 28.92%, and the monoglyceride content was 42.06%. Under the condition that the temperature is 140°C and the pressure is 10Pa, molecular distillation ...

Embodiment 2

[0034] Embodiment 2 (solvent-free system)

[0035] Take 5g of XAD1180 resin, 10g of Tris-HCL buffer, and 1g of free lipase MAS1-H108W, mix and stir at 30°C for 3 hours, then filter and dry to obtain immobilized lipase MAS1-H108W, and put it in a 4°C refrigerator for later use.

[0036]Take 7.62g of soybean oil, 2.38g of glycerol, and 1g of the above-mentioned fixed lipase MAS1-H108W in a flask, place them in an air bath shaker, and set the temperature at 50°C with the speed of the air bath shaker at 150rpm / min After reacting for 4 hours, stop the reaction. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 17.9%, the diglyceride content was 28.2%, and the monoglyceride content was 53.9%. Under the conditions of temperature 150°C and pressure 13Pa, molecular distillation is carr...

Embodiment 3

[0037] Embodiment 3 (solvent-free system)

[0038] Take 10g of ECR8806 resin, 20g of Tris-HCL buffer, and 1.5g of free lipase MAS1-V233F, mix and stir at 35°C for 4 hours, then filter and dry to obtain immobilized lipase MAS1-V233F, and put it in a 4°C refrigerator for later use.

[0039] Take 6.58g of soybean oil, 3.42g of glycerol, and 1g of the above-mentioned fixed lipase MAS1-V233F in a flask, place them in an air bath shaker, and set the temperature at 60°C with the speed of the air bath shaker at 150rpm / min After reacting for 6 hours, stop the reaction. The reactant was poured into a centrifuge tube and centrifuged at 10,000 rpm / min for 3 minutes to obtain the upper oil phase, which was a mixture rich in partial glycerides. The upper oil phase was taken for detection, and the triglyceride content was 20.4%, the diglyceride content was 30.8%, and the monoglyceride content was 48.8%. Under the condition that the temperature is 150°C and the pressure is 13Pa, molecular d...

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PUM

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Abstract

The invention discloses a method for preparing partial glyceride through a glycerolysis reaction. The method comprises the following steps: (1) preparing immobilized lipase of a Lipase MAS1 mutant, wherein the Lipase MAS1 mutant is MAS1-H108A, and the amino acid sequence of the Lipase MAS1 mutant is as shown in SEQ NO. 2; (2) preparing partial glyceride by enzyme catalysis glycerolysis reaction: mixing 1-10 parts by mass of vegetable oil, 1-4 parts by mass of glycerol, 0-40 parts by mass of organic phase reagent and 0.2-1.5 parts by mass of immobilized lipase, and reacting for 1-6 hours at the temperature of 40-55 DEG C to obtain a product mixture; and (3) separating the product mixture to obtain the partial glyceride. The method has the advantages of simple process, high reaction rate, high product content and no side reaction.

Description

technical field [0001] The invention relates to a method for preparing partial glycerides by glycerolysis reaction. Background technique [0002] Partial glyceride is a general term for diglyceride and monoglyceride, and both diglyceride and monoglyceride can be used as food additives. Monoglycerides can be used as emulsifiers in the fields of food, medicine and cosmetics, while diglycerides have been certified as a new type of healthy oil that is safe, non-toxic and side effects, and can effectively prevent fat accumulation and regulate body fat. Generally speaking, the production of partial glycerides can be divided into chemical method and biological enzymatic method. Although the chemical method is low in cost and easy to carry out large-scale production, it has the disadvantages of generating many by-products, large pollution, and high energy consumption for the reaction. The emergence of the biological enzyme method has solved these problems very well. The production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/08C12N11/082C12N9/20
CPCC12P7/6445C12N11/08C12N9/20C12Y301/01003
Inventor 王永华岳海鹏杨博王卫飞蓝东明罗日明
Owner YHSN TECH LTD
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