Common fifty-five respiratory tract pathogen high-throughput identification and detection method

Abstract

The invention discloses a common fifty-five respiratory tract pathogen high-throughput identification and detection method which comprises the following steps: detecting whether a target nucleic acidexists or not only by detecting whether a single base extension reaction occurs at a polymorphic site or not without determining the base type of the polymorphic site so as to judge whether a sample target pathogen exists or not; the method specifically comprises the following steps: multiplex PCR, single base extension reaction and mass spectrum detection. The method has the beneficial effects that the diagnosis efficiency is improved, the method is not prone to cross contamination, the test operation is simple and convenient, standardization is easy, and various common respiratory virus nucleic acids in clinical specimens can be rapidly detected at the same time. High-throughput differential diagnosis is carried out on the common respiratory tract pathogens so that the pathogens can be clarified clinically as soon as possible, and the method has important significance in targeted anti-infection treatment.

Description

technical field [0001] The invention belongs to the technical field of detection of respiratory pathogens, in particular to a high-throughput identification and detection method for fifty-five kinds of common respiratory pathogens. Background technique [0002] Respiratory tract infection has caused a serious disease burden to human beings and poses a great threat to human life and health. There are a wide variety of pathogens that can cause respiratory-like infections. According to the statistics released by WHO in 2018, respiratory tract infection is the fourth leading cause of death in the world and the third leading cause of death of children under 5 years old in China, accounting for 19.4% of all causes of death. The pathogens include viruses, bacteria, mycoplasma and chlamydia. Among the pathogens infected by children, viruses accounted for more than 70%. 20-60% of CAP cases of community-acquired pneumonia in China cannot be etiologically diagnosed. [0003] Common ...

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Problems solved by technology

[0006] There are significant differences in the susceptibility pathogenic spectrum of different types of pneumonia and people with different immune status, which cannot be generalized. For example, viral pneumonia is more common in children under 5 years old, while bacterial pneumonia is more common in adults, especially the elderly. The symptoms of infection caused by different pathogens are very similar, often showing the situation of "one disease with multiple causes", which brings great difficulties to the diagnosis of respiratory pathogens

[0007] At present, the methods for diagnosing respiratory infections are mainly pathogen isolation and culture methods and serological detection methods. The former is usually considered the "gold standard" for diagnosis. Diagnosis: Indirect immunofluorescence (IFA) is one of the serological detection methods. This method is convenient and easy, and requires the specimen to contain a certain amount of antigen and antibody. The detection is fast, but the sensitivity or specificity is not high, and false positives are prone to occur and false negatives, and due to the existence of the window period, it is also impossible to diagnose early in the infection

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