Latex microspheres for improving stability of latex immunoturbidimetry, preparation method and application

Abstract

The invention relates to the technical field of polymer microsphere materials, and specifically discloses a preparation method of latex microspheres for improving the stability of latex immunoturbidimetry. The preparation method comprises the following steps of: mixing p-4-chloromethoxystyrene containing chloromethoxy as a raw material, introducing nitrogen, stirring for pre-emulsifying, adding potassium persulfate, heating, and continuing to stir for reaction, so as to obtain a suspension of the latex microspheres; and dropwise adding a lithium chloride aqueous solution for demulsifying, andcarrying out reduced pressure suction filtration, washing and centrifuging to obtain latex microspheres. A styrene derivative with chloromethoxy is added in polymerization raw material selection, so that the polymerization conversion rate can be improved, the hydration diameter and surface negative charges of the latex microspheres are reduced, and the stability of the microspheres is improved. Alatex reagent prepared from the latex microsphere provided by the invention has a better effect and better stability during immunoturbidimetry detection.

Description

technical field [0001] The invention relates to the technical field of polymer microsphere materials, in particular to a latex microsphere for improving the stability of latex immunoturbidimetric method, a preparation method and an application. Background technique [0002] At present, the latex-enhanced immunoturbidimetric method can be widely used in the detection of special proteins, tumor markers, etc., and is a new generation of practical immunoassay technology with great development prospects. The in vitro diagnostic biochemical products of latex-enhanced immunoturbidimetric method in the market mainly include CysC, RBP, β2-MG, Lp(a), hsCRP, ASO, RF and other detection kits. [0003] Latex reagent is a liquid, and its various performance indicators have high requirements for its stability, which is also an important and difficult point in the development of latex reagents. The stability of latex reagents mainly includes two aspects, one is the stability of latex micro...

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Problems solved by technology

[0004] reference document CN110836965A discloses a liquid chip blocking solution and its method, the blocking solution includes: phosphate buffer, protein, amino acid, surfactant Agents and preservatives can reduce non-specific binding, improve sensitivity and precision, but the blocking agent in this document is not fully suitable for latex immunoturbidimetric method; a kind of improved latex-bound antigen or antibody stability is disclosed in the comparative document CN101046473A method, the method described is to add blocking agent and stability to the reagent, this method can make the reagent absorbance value change little in the long-term storage of the reagent, but the reagent prepared by this method will decrease in sensitivity and accuracy with the prolongation of time and precision performance gradually degrades

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