Pediococcus acidilactici with excellent alcohol stress resistance and application

A kind of Pediococcus lactis, anti-alcohol technology, applied in the field of microorganisms, can solve the problems such as patent publication documents have not been found, and achieve the effect of strong anti-alcohol stress ability

Active Publication Date: 2020-11-17
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Through the search, no patent publications related to

Method used

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  • Pediococcus acidilactici with excellent alcohol stress resistance and application
  • Pediococcus acidilactici with excellent alcohol stress resistance and application
  • Pediococcus acidilactici with excellent alcohol stress resistance and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The isolation and identification of embodiment 1 lactic acid bacteria

[0034] The following experiments were performed under sterile conditions.

[0035] 1. Enrichment of strains

[0036] Weigh 25g of fermented grains during the brewing process of liquor, add 225mL of sterile saline and mix well, and dilute the obtained bacterial solution by 10 3 ~10 5 After doubling, take 100 μL and spread it on the MRS plate, incubate upside down at 37°C for 24 hours, and observe the growth of the colony.

[0037] The preparation method of the MRS solid medium is: peptone 10g, beef extract powder 5g, Tween 80 1mL, dipotassium hydrogen phosphate 2g, yeast extract powder 4g, magnesium sulfate 0.2g, triammonium citrate 2g, glucose 20g, Manganese sulfate 0.05g, sodium acetate 5g, agar 20g, distilled water 1L, pH 6.2±0.2, sterilized at 121°C for 20min for later use.

[0038] 2. Isolation of strains

[0039] Pick the colonies obtained in step 1, carry out microscopic examination with ...

Embodiment 2

[0046] Example 2 Tolerance of Pediococcus lactis CGMCC No.20087 under different concentrations of alcohol treatment

[0047] Select the strains at the end of logarithmic growth, add different contents (4%, 6%, 8%, 10%, 12%) of alcohol to the MRS liquid medium with 2% inoculum size, and carry out alcohol stress in a 37 ° C incubator for 12 h , and the MRS medium culture group without alcohol was used as a negative control. OD was measured after stress 600nm Compare their growth status.

[0048] The preparation method of described MRS liquid medium is: peptone 10g, beef extract powder 5g, Tween 80 1mL, dipotassium hydrogen phosphate 2g, yeast extract powder 4g, magnesium sulfate 0.2g, triammonium citrate 2g, glucose 20g, Manganese sulfate 0.05g, sodium acetate 5g, distilled water 1L, pH 6.2±0.2, sterilized at 121°C for 20min for later use.

[0049] The pure bacteria obtained in Example 1 were treated with different concentrations of alcohol. With the increase of alcohol conce...

Embodiment 3

[0050] The characteristic determination of embodiment 3 Pediococcus lactis CGMCC No.20087

[0051] 1. Determination of acid resistance

[0052] The strains at the end of logarithmic growth were selected, and 2% inoculum was added to liquid MRS medium with a pH of 3.0 and cultured in a 37°C incubator for 4 hours, while the MRS medium culture group with conventional pH was used as a negative control. OD was measured after stress 600nm Compare their growth status.

[0053] 2. Determination of bile salt tolerance

[0054] The strains at the end of logarithmic growth were selected, and 2% of the inoculum was added to the liquid MRS medium containing 0.3% bile salts and cultured in a 37°C incubator for 4 hours, and the conventional MRS medium culture group was used as a negative control. OD was measured after stress 600nm Compare their growth status.

[0055] 3. Determination of adhesion ability

[0056] Add rat tail collagen to the 96-well plate, shake open the lid and let it...

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Abstract

The invention relates to Pediococcus acidilactici with excellent alcohol stress resistance. The name of pediococcus acidilactici is LTJ28, the classification name is pediococcus acidilactici, the preservation number is CGMCC No.20087, the preservation date is June 15, 2020, in No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing, and the preservation unit is China General Microbiological Culture Collection Center. The pediococcus acidilactici disclosed by the invention is derived from fermented grains in the fermentation process of traditional fermented food white spirit, and belongs toa universally accepted safe strain which can be used for food. The pediococcus acidilactici has excellent alcohol stress resistance, and the pediococcus acidilactici is a dominant strain in white spirit fermented grains and has strong alcohol stress resistance.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a strain of Pediococcus lactis with excellent resistance to alcohol stress and its application. Background technique [0002] Lactic acid bacteria is a general term for a class of bacteria that can ferment carbohydrates to produce lactic acid. Its Gram staining is positive, and its shape is mostly rod-shaped or spherical. The excellent lactic acid bacteria required in the production of fermentation should have good fermentation performance and strong tolerance to ensure that the microorganisms can survive in the human gastrointestinal tract after the fermented products are eaten, which is also the key to the role of probiotic lactic acid bacteria. [0003] Pediococcus acidilactici is a kind of lactic acid bacteria. The bacteriocin produced by it has inhibitory effect on many Gram-positive bacteria and some Gram-negative bacteria, showing a broad antibacterial spectrum. The bact...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/744A61P25/32A23L33/135C12R1/01
CPCA61K35/744A61P25/32A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/413A23V2200/334
Inventor 罗学刚王佳丽孟仪方廖子萱胡艳红
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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