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Method for positioning pig meat color trait whole genome methylation site based on CpG

A genome-wide, positioning method technology, applied in the field of molecular biology, to achieve the effect of accurate identification, high reliability, important economic benefits and social value

Inactive Publication Date: 2020-11-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many studies related to epigenetics in pigs, but most studies focus on differential methylation analysis and non-coding RNA, and few studies use whole gene technology to analyze the association between epigenetic variation and traits , and further reveal the relationship between phenotypic traits and epigenetic variation

Method used

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  • Method for positioning pig meat color trait whole genome methylation site based on CpG
  • Method for positioning pig meat color trait whole genome methylation site based on CpG

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Embodiment

[0034] A method for mapping methylation sites in the whole genome of pork color traits based on CpG, comprising the following steps:

[0035] S1. Select 140 experimental pigs;

[0036] S2, correcting the phenotype data of meat color traits of 140 experimental pigs;

[0037] S3, collect the muscle tissue sample of experimental pig, extract genomic DNA, figure 1 Electropherogram of genomic DNA;

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Abstract

The invention discloses a method for positioning a pig meat color trait whole genome methylation site based on CpG. The methylation site is based on a chromosome No.12 of a reference sequence of a version 11.1 of an international pig reference genome, and the methylation level of the methylation site is high. The CpG methylation site provided by the invention is remarkably related to the meat color trait yellowness value of a large white pig, so that the large white pig with good meat color stability can be screened by identifying CpG methylation of the site, and then commercial pig meat withgood meat quality is obtained; and therefore, the method has important economic benefits and social values.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a CpG-based positioning method for whole-genome methylation sites of pork color traits. Background technique [0002] The purpose of the pig industry is to meet people's ever-increasing consumer demand for pork and its products, which includes both the demand for its quantity and the demand for its quality. In the process of pig genetics and breeding, pig production performance has always been the main breeding goal, and remarkable results have been achieved. However, in recent years, with the development of my country's large-scale pig farming industry and the improvement of people's living standards, consumers' demand for high-quality pork has become more and more urgent, and the quality of pork has attracted more and more attention. Meat color is one of the most important measurement indicators in meat quality traits, an external manifestation of muscle physiology, biochemist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858G16B30/10
CPCC12Q1/6888C12Q1/6858G16B30/10C12Q2600/124C12Q2600/154C12Q2537/165
Inventor 唐国庆王凯吴平先周洁陈德娟杨喜堤
Owner SICHUAN AGRI UNIV