SSR primer and method for identifying purity of hybrid seeds of Zaojia Luffa cylindrica

Abstract

The invention belongs to the technical field of molecular detection, and particularly relates to an SSR primer and method for identifying the purity of hybrid seeds of Zaojia Luffa cylindrica. The method disclosed by the invention can be used for detecting the purity of the hybrid seeds of the Zaojia Luffa cylindrica, and can effectively distinguish the hybrid seeds from a male parent and a femaleparent to quickly detect the purity of the hybrid seeds. The method has the advantages of simplicity, rapidness, accuracy, low cost, simplicity in operation and the like; by adopting the method, oneperson can complete the detection of nearly 500 samples in one day; and a traditional method for identifying the purity of the hybrid seeds of the Luffa cylindrica can be replaced, the operation is simple and convenient, labor is saved, land does not need to be occupied, a result is accurate and reliable, the identification efficiency is greatly improved, and the identification cost is reduced, sothat the method has relatively high commercial application value.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to an SSR primer and a method for identifying the purity of Zaojia luffa hybrid seeds. Background technique [0002] Loofah is a cultivated species in the genus Luffa of Cucurbitaceae, an annual climbing herb. There are two species of loofah cultivated in my country, namely common loofah (Luffa cylindrica (L.) M.J. Roem.) and ribbed loofah (Luffa acutangula (L.) Roxb.). Zaojia loofah belongs to the meat loofah in common loofah (Luffa cylindrica (L.) M.J.Roem.). In the seed production process of loofah hybrids, the method of detasseling the female parent is mainly used. That is, the parents are sown separately according to the growth period, and all the male flower buds of the female parent are manually removed before the female flower blooms. During the flowering period, the pollen of the male parent is collected and pollinated on the stigma of the female ...

AI Technical Summary

Problems solved by technology

[0003] The traditional purity detection of loofah hybrids mainly adopts morphological identification, which is mainly based on the morphological identification of the seeds and ovaries of the female parent and hybrids. This method is practical, but the identification time takes about 60 days, which has a long cycle and occupies an area of Defects such as large and greatly affected by the environment

At present, the use of molecular markers to identify the purity of hybrids has the advantages of good stability and large amount of information, and has been widely used in the detection of crop seed purity and quality. No. 2" and common loofah "Sisheng No. 2" hybrids were reported, but the primers and methods for the purity identification of Zaojia loofah have not been reported yet.

At the same time, at present, there are still problems in the identification of the purity of hybrids by SSR molecular markers in loofah: (1) Genome extraction adopts CTAB method or SOD method, and the operation steps are cumbersome and time-consuming; (2) The unoptimized PCR reaction system and procedures waste costs; (3) ) silver staining step dyeing and color development link operation is cumbersome, unstable results and other technical problems

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